Detection of Some Bacteriocin Genes in Enterococcus faecium Isolates Obtained from Mastitic Bovine Milk Samples

Abstract

In this study, Enterococcus faeciumisolates obtained from mastitis cattle were investigated for carrying the most important bacteriocin structural genes enterocin A (entA), enterocin B (entB), enterocin P (entP) genes. The study material was 620 milk samples taken from mastitic cattle. Enterococcal isolation was performed using classical bacteriological methods in selective media. Molecular methods were used to in order to conduct the identification of genus and species as well as the presence of enterocin genes. As a result of isolation studies, 15.5% (96 isolates) of enterococci were isolated; of which 13.5% (13 isolates) were E. faecium. entA and entB genes bacteriocin structural genes were determined to be 30.8% (4 isolates) and the entP gene 7.7% (1 isolate). There were two gene combinations: the first one with 23.0% (3 isolates) entA + entB gene combination and the second with 7.7% (1 isolate) entA + entB + entP genes. This study showed that E. faeciumstrains obtained from mastitic cattle milk could potentially synthesize bacteriocin because they have potency to carry bacteriocin genes. It is believed that further studies are needed to determine the distribution of bacteriocin genes in other enterococci isolated from bovine milk of mastitis and to search for potential use of bacteriocins in this area.

Keywords

• Enterococcus faecium,Bovine,Mastitis,Bacteriocin,Gene

Mastitisli Sığır Süt Örneklerinden Elde Edilen Enterococcus faecium İzolatlarında Bazı Bakteriyosin Genlerinin Saptanması

Abstract

Bu çalışmada mastitisli sığırlardan elde edilen Enterococcus faeciumizolatlarının, en önemli bakteriyosin yapısal genleri olan enterosin A (entA), enterosin B (entB), enterosin P (entP) genlerini taşıma potansiyelleri araştırıldı. Çalışma materyalini 620 mastitisli sığırdan alınan süt örnekleri oluşturdu. Enterokok izolasyonu selektif besiyerinde klasik yöntemler kullanılarak gerçekleştirildi. Cins ve tür düzeyinde identifikasyon ile bakteriyosin gen varlıklarını belirlemek için moleküler yöntemler kullanıldı. İzolasyon çalışmaları sonucunda %15.5 (96 izolat) enterokok izole edilirken; bunların %13.5 (13 izolat)’unun E. faecium olduğu belirlendi. Bakteriyosin yapısal genlerinden entA ve entB genleri izolatların %30.8 (4 izolat)’inde belirlenirken; entP geni %7.7 (1 izolatta) bulunuyordu. İki gen kombinasyonu mevcuttu: Bunlardan birincisi %23.0 (3 izolat) oranında entA+entB gen kombinasyou ikincisi ise %7.7 (1 izolat) oranında entA+entB+entP genlerinin birlikte görüldüğü kombinasyondu. Bu çalışma ile mastitisli sığır sütlerinden elde edilen E. faeciumsuşlarının bakteriyosin genlerini taşıma potansiyeline sahip oldukları dolayısı ile bakteriyosin sentezleyebilecekleri belirlendi. Mastitis sığır sütünden izole edilen diğer enterokoklarda bakteriyosin genlerinin dağılımını saptamak ve bakteriyosinlerin bu alanda kullanım potansiyelini aramak için daha fazla çalışma yapılmasına ihtiyaç duyulduğuna inanılmaktadır.

Keywords

• Enterococcus faecium,Sığır,Mastitis,Bakteriyosin,Gen

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