Existence of Plasmidic AmpC Beta-Lactamase-Producing Escherichia coli Isolates in Healthy Laying Hens

Abstract

In the present study, determination of prevalence of plasmidic AmpC (pAmpC) beta-lactamase-producing Escherichia coli in intestinal flora of laying hens in Burdur province of Turkey and characterization of pAmpC beta-lactamase-producing E. coli isolates were aimed. Two hundreds twenty five fecal samples from all laying hen farms (n=4) in Burdur province were collected and cultured in Brilliance E. coli/coliform selective agar supplemented with cefotaxime or ceftazidime. Presumptive AmpC beta-lactamase-producing E. coli isolates were determined by a phenotypic test and the isolates were screened by PCR for bla_pAmpC genes. Susceptibilities of the E. coli isolates to beta-lactams and other classes of antibiotics were investigated by agar disc diffusion test and finally phylogenetic analysis of the E. coli isolates was performed by multiplex PCR. pAmpC beta-lactamase-producing E. coli was isolated from 15 (6.7%) laying hen fecal samples. bla_CITM family gene was found in all E. coli isolates. The pAmpC beta-lactamase-producing isolates showed co-resistance to several classes of antibiotics (aminoglycosides, quinolones, sulfamethoxazole-trimethoprim and tetracycline). According to phylogenetic analysis, the E. coli isolates belonged to A₁, B1 and D₁ groups. Consequently, by the present study the first isolation of pAmpC beta-lactamase-producing E. coli isolates with multidrug-resistance phenotype on lying hen production from Turkey was reported.

Keywords

• Chicken,Escherichia coli,Multiple antibacterial drug resistance,AmpC beta-lactamases

Sağlıklı Yumurta Tavuklarında Plasmid Kökenli AmpC Beta Laktamaz üreten Escherichia coli Varlığı

Abstract

u çalışmada, Burdur ilinde yetiştirilen yumurtacı tavukların bağırsak mikroflorasında plazmid kökenli AmpC (pAmpC) beta laktamaz üreten Escherichia coli izolatlarının prevalansını belirlemek ve belirlenen pAmpC beta laktamaz üreten E. coli izolatlarının karakterizasyonunun yapılması amaçlandı. Burdur ilindeki tüm yumurtacı tavuk çiftliklerinden (n=4) 225 dışkı örneği toplandı ve içerisine sefotaksim veya seftazidim ilave edilerek hazırlanmış E. coli/koliform selektif agarda dışkı örneklerinin kültürleri yapıldı. AmpC beta laktamaz üreten E. coli izolatlarının ön tanısı fenotipik bir test ile yapıldı ve izolatlar bla_pAmpC beta laktamaz genleri için PZR ile tarandı. E. coli izolatlarının beta laktam ve diğer sınıflardan antibiyotiklere olan duyarlılıkları agar disk difüzyon testi ile araştırıldı ve son olarak E. coli izolatlarının filogenetik analizi multipleks PZR ile gerçekleştirildi. pAmpC beta laktamaz üreten E. coli 15 adet (%6.7) dışkı örneğinden izole edildi. CIT familyasına ait bla_pAmpC geni (bla_CIT) tüm E. coli izolatlarında bulundu. pAmpC beta laktamaz üreten izolatlar çeşitli sınıflardan antibiyotiklere (aminoglikozidler, kinolonlar, sulfametoksazol-trimethoprim ve tetrasiklinler) karşı dirençli bulundu. Filogenetik analiz sonuçlarına göre E. coli izolatları A₁, B1 ve D₁ gruplarına ait oldukları belirlendi. Sonuç olarak, Türkiye’de yumurtacı tavuk üretiminde pAmpC beta laktamaz üreten ve çoklu antibiyotik direnci gösteren E. coli izolatlarının ilk kez bu çalışmayla ortaya konulduğu görüldü.

Keywords

• Tavuk,Escherichia coli,Çoklu antibakteryel ilaç dirençliliği,AmpC beta laktamazlar

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