The Effect of Enrofloxacin and Caffeic Acid Phenethyl Ester on Oxidant and Antioxidant Status in the Blood in Ornithobacterium rhinotracheale Infection

Abstract

The effects of enrofloxacin and caffeic acid phenethyl ester (CAPE) alone and in combination applications on the free radical damage and antioxidant system changes occurring in the blood of broilers in Ornithobacterium rhinotracheale (ORT) infection were investigated. For this study, 96 broilers aged 4 weeks were divided in 8 groups. These 8 groups are respectively, I. Control Group, II. Antibiotic Group, III. CAPE Group, IV. Antibiotic + CAPE Group, V.  ORT Group, VI. ORT + Antibiotic Group, VII. ORT + CAPE Group, VIII. ORT + CAPE + Antibiotic Group. An inoculum of the ORT 3263/91 strain (serotype A) containing 3.8 x 10⁸ CFU mL^-1 was given as aerosol to the groups V-VIII. For groups, CAPE and Antibiotic applications were started at the same time on 17th day after ORT applications. For 6 days, enrofloxacin (10 mg/kg/day) was added to the drinking water of the groups II, IV, VI, VIII and CAPE (10µmol/kg) was implemented as i.p to the groups III, IV, VII, VIII. As a result, infection of ORT increased the levels of serum MDA and β-karoten (p<0.01), and decreased the activities of GSH-Px ve CAT (p<0.001) from the antioxidant enzymes. Given CAPE alone and with antibiotic to the control group causes to raise the level of MDA (p<0.05). While antibiotic, CAPE, and antibiotic + CAPE applications caused a significant reduction on the activity of CAT (p<0.01), the activities of GR (p<0.05) and GSH-Px (p<0.01) were increased.  Applying antibiotic and CAPE separately to the control group causes to reduce the level of NO (p<0.05). Application of antibiotic and CAPE separately to the ORT group caused to decrease with statistically insignificant in the level of MDA. Applications of antibiotic, CAPE, and antibiotic + CAPE caused to reduce in the CAT activity (p<0.05), alone CAPE and together with antibiotic caused to reduce in ALT activity (p<0.001), and alone CAPE caused to reduce in level of Vit E (p<0.05). Applications of alone antibiotic and CAPE caused to reduce in the AST activity, when they were applied together the decrease in the level of AST was found statistically significant (p<0.05). Also, applications of alone KAFE and together with antibiotic caused a significant increase in the GR activity (p<0.01). The application of CAPE in the ORT infection has beneficial effects on the systemic oxidant and antioxidant balance.

Keywords

• Ornithobacterium rhinotracheale,Enrofloxacin,Caffeic acid phenethyl ester,Blood,Oxidant-antioxidant status

Ornithobacterium rhinotracheale Enfeksiyonunda Kandaki Oksidan ve Antioksidan Durum Üzerine Enrofloksasin ve Kafeik Asit Fenetil Ester’in Etkisi

Abstract

Ornithobacterium rhinotracheale (ORT) infeksiyonunda broylerlerin kanında meydana gelen serbest radikal hasarı ve antioksidan sistemdeki değişim üzerine enrofloksasin ve kafeik asit fenetil ester (KAFE)’nin tek başına ve birlikte uygulanmasının etkilerinin araştırılması amaçlanmıştır. 4 haftalık 96 adet civciv 8 gruba bölündü. I. Kontrol Grubu, II. Antibiyotik Grubu, III. KAFE Grubu, IV. Antibiyotik + KAFE Grubu, V. ORT Grubu, VI. ORT + Antibiyotik Grubu, VII. ORT + KAFE Grubu ve VIII. ORT + Antibiyotik + KAFE Grubu. ORT 3263/91 suşu (serotip A) mililitre başına 3.8x10⁸ CFU içeren inokulum aerosol olarak verildi (Grup V-VIII). Gruplara antibiyotik ve KAFE uygulanmasına ORT verilmesinden sonraki 17. günde aynı zamanda başlatıldı. 6 gün süre ile enrofloksasin (10 mg/kg/gün) içme sularına katıldı (Grup II, IV, VI, VIII), KAFE (10 µmol/kg) i.p olarak uygulandı (Grup III, IV, VII, VIII). ORT infeksiyonu serum MDA ve β-karoten düzeyinde artışa (p<0.01), antioksidan enzimlerden GSH-Px ve KAT aktivitesinde azalmaya (p<0.001) neden olmuştur. Kontrol grubuna tek başına KAFE’nin veya Ant ve KAFE’nin birlikte verilmesi MDA düzeyinde artışa sebep olmuştur (p<0.05). Yine Ant, KAFE, Ant + KAFE uygulaması KAT aktivitesinde önemli azalmaya (p<0.01) neden olurken, GR (p<0.05) ve GSH-Px (p<0.01) aktivitesinde ise artışa neden olmuştur. Kontrol grubuna tek başına Ant ve KAFE uygulanması NO düzeyinde önemli azalmaya (p<0.05) sebep olmuştur. ORT grubunda tek başına Ant ve KAFE’nin uygulanması MDA artışında istatistiksel olarak anlamlı olmayan bir düşüşe neden olmuştur. Ant, KAFE, Ant+KAFE uygulaması KAT aktivitesinde (p<0.05), tek başına KAFE veya Ant ve KAFE’nin birlikte verilmesi ALT aktivitesinde (p<0.001) ve tek başına KAFE Vit E düzeyinde (p<0.05) önemli azalmaya sebep olmuştur. Ant ve KAFE tek başına AST aktivitesinde azalmaya sebep olmuş, Ant ve KAFE birlikte verildiği zaman bu azalma istatistiki olarak anlamlı bulunmuştur (p<0.05). Ayrıca tek başına KAFE veya Ant ve KAFE’nin birlikte verilmesi GR aktivitesinde ise önemli artışa neden olmuştur (p<0.01). ORT enfeksiyonunda tek başına KAFE uygulamasının sistemik oksidan-antioksidan denge üzerine olumlu etkileri vardır.

Keywords

• Ornithobacterium rhinotracheale,Enrofloksasin,Kafeik asit fenetil ester,Kan,Oksidant-antioksidant durum

References

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