Determination of Serum Lipid Profile and Lipoprotein Levels of Sheep with Naturally Acute Babesiosis

Abstract

In this study, which was done to determine the lipid profile and the variations about the levels of lipoprotein in the blood of sheep naturally infected with Babesia ovis, 3-5 year old, 30 infected and 15 healthy, totally 45 Morkaraman breed sheep raised in Erciş county of Van city were used as materials. The measurement of triglyceride, total cholesterol, LDL and HDL were studied by using enzymatic colorimetric method. VLDL levels were calculated by formula. The percentages of free fatty acids, triglyceride, phospholipids, cholesterol and cholesteryl esters were measured by using thin layer chromatography. In the infected group it was determined that the levels of triglyceride (P<0.001) and VLDL (P<0.001) statistically increased, but the HDL (P<0.05) decreased compared to control group. After the chromatographic determination of lipid amounts (%), in the infected group it was determined that triglyceride amount (P<0.001) statistically increased, but phospholipids (p<0.05) and cholesterol esters amounts (P<0.01) decreased compared to control group. As a result, it is said to be important that lipid levels in sheep babesiosis and lipoprotein levels are important for animals in terms of prognosis.

Keywords

• Babesia ovis,Cholesterol,HDL,LDL,Triglyceride,VLDL

Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi

Abstract

Babesia ovis ile doğal enfekte olan koyunlarda kandaki lipit profilini ve lipoprotein düzeylerindeki değişimi saptamak için yapılan bu çalışmada canlı materyal olarak Van ili Erciş ilçesinde yetiştirilen 3-5 yaşları arasındaki, toplam 45 Morkaraman ırkı koyun kullanıldı (30 enfekte ve 15 sağlıklı koyun). Serum örneklerinde trigliserit, total kolesterol, LDL ve HDL ölçümü enzimatik kolorimetrik yöntem kullanılarak çalışıldı. VLDL düzeyleri formülle hesaplandı. Serbest yağ asitleri, trigliserit, fosfolipit, kolesterol ve kolesterol esterlerinin % miktarları ise ince tabaka kromatografisi kullanılarak ölçüldü. Enfekte hayvanlarda sağlıklı kontrollere göre trigliserit (P<0.001) ve VLDL düzeylerinin (P<0.001) istatistik olarak önemle arttığı, HDL düzeyinin (P<0.05) ise azaldığı tespit edildi. Lipit miktarlarının (%) kromatografik olarak incelenmesi sonucunda enfekte hayvanlarda sağlıklı kontrollere göre trigliserit miktarının (P<0.001) istatistik olarak önemle arttığı, fosfolipit (P<0.05) ve kolesterol esterlerinin miktarının (P<0.01) ise azaldığı saptandı. Sonuç olarak, koyun babesiosisinde lipit profilinin ve lipoprotein düzeylerinin bilinmesinin hayvanlarda prognoz açısından önemli olduğu söylenebilir.

Keywords

• Babesia ovis,Kolesterol,HDL,LDL,Trigliserit,VLDL

References

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