Histological and Histometric Structure of Goose (Anser anser) Cerebellum

Abstract

The aim of this study is to examine the goose (Anser anser) cerebellum histometric and histologically. Six female geese (aged between 10-12 months) were used in this study. After fixation in 10% formalin and following routine histologic processes, the cerebellar tissues of geese were embedded in paraffin blocks and the sections were taken from these blocks. The Kluver-Barrera nerve tissue stain technique was applied to the sections. The ocular micrometer was used for histometric measurements of folia length, diameter of purkinje cells and thicknesses of stratum granulosum and stratum moleculare. SPSS 16.0 was used for calculating mean values. It was observed histological that folia and sulci cerebelli were in the outermost layer of the cerebellum. As a result of Kluver-Barrera staining, the nerve cells in all layers appeared purple and myelin fibers appeared blue. It was found that the average length of the folia was 3397.88 ± 931.32 µm, the average thickness of molecular layer was 348.53 ± 72.92 µm, the average thickness of granular layer was 184.83 ± 48.77 µm and the average diameter of Purkinje cells was 24.23 ± 3.96 µm.

Keywords

• Cerebellum,Goose,Kluver-Barrera,Purkinje cell

Kaz (Anser anser) Beyinciğinin Histolojik ve Histometrik Yapısı

Abstract

Bu çalışmada, kaz (Anser anser) serebellum dokusunun histolojik ve histometrik yapısının incelenmesi amaçlandı. Çalışmada 6 adet dişi kaz (10-12 aylık) kullanıldı. Kazlara ait serebellum dokuları % 10’luk formalinde tespit edildikten sonra rutin histolojik işlemlerden geçirilip parafinde bloklandı ve bu bloklardan kesitler alındı. Kesitlere Kluver-Barrera sinir doku boyama tekniği uygulandı.  Folia uzunluğu, Purkinje hücre çapı, stratum molekülare ve stratum granülosum katmanlarının kalınlıklarını ölçmek için oküler mikrometre kullanıldı. Ortalama değerleri hesaplamak için SPSS 16.0 programı kullanıldı. Yapılan histolojik incelemelerde serebellumun dış kısmında folia ve sulkus serebelli yapıları gözlendi. Kluver-Barrera boyaması sonucunda tüm katmanlardaki sinir hücreleri mor renkte, miyelinli lifler ise mavi renkte görüldü. Foliaların ortalama uzunluğu 3397.88 ± 931.32 µm, stratum molekülare katmanının ortalama kalınlığı 348.53 ± 72.92 µm, stratum granülosum katmanının ortalama kalınlığı 184.83 ± 48.77 µm ve Purkinje hücrelerinin ortalama çapı ise 24.23 ± 3.96 µm olarak belirlendi.

Keywords

• Kaz,Kluver-Barrera,Purkinje hücresi,Serebellum

References

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