The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal Swab Samples from Dogs in Van Province, Turkey

Abstract

In this study, it was aimed to determine the presence of CPV infection in the blood and rectal swaps of suspected dogs that they were handed to Yuzuncu Yıl University, Faculty of Veterinary, Department of Internal Disease using PCR techniques and molecular characterization of local viruses. 57 (75%) of the 76 rectal swabs used in the study and 40 (64%) of the 62 blood samples were determined to be positive by PCR obtained from dogs with clinical symptoms. Selected samples were positive with the virus carried the molecular characterization. The results of the sequence analysis were similar among themselves and they were found in CPV-2a and CPV-2b groups. It was determined that the sequences of virus strains showed 99-100% homology with other strains obtained from Genbank.

Keywords

• CPV,PCR,Molecular characterization,Phylogenetic analysis

Türkiyede Van Yöresinde Bulunan Köpeklerden Sağlanan Kan ve Rektal Sıvap Örneklerinde Canine Parvovirus-2 Enfeksiyonunun Araştırılması ve Filogenetik Analizi

Abstract

Bu çalışmada, Van ili Yüzüncü Yıl Üniversitesi Veteriner Fakültesi İç Hastalıkları Kliniğine getirilen Canine Parvovirus (CPV) Enfeksiyonu şüpheli köpeklerden alınan kan ve rektal sıvap örneklerinde PZR tekniği kullanılarak CPV enfeksiyonunun varlığının belirlenmesi ve yerel virusların moleküler karakterizasyonunun yapılması amaçlandı. Klinik olarak enfeksiyon belirtisi gösteren köpeklerden sağlanan 76 adet rektal sıvaptan 57 adedi (%75), 62 adet kan örneğinin ise 40 adedi (%64) PZR ile pozitif olarak belirlendi. Seçilen pozitif örnekler ile virusun moleküler karakterizasyonu gerçekleştirildi. Elde edilen sekans sonucu bilgileri değerlendirildiğinde örneklerin CPV-2a ve CPV-2b grublarında bulunduğu ve kendi aralarında benzerlik gösterdikleri belirlendi. Elde edilen virus suşu sekanslarının Genbankasından sağlanan diğer suşlarla %99-100 oranında homoloji gösterdiği belirlendi.

Keywords

• CPV,PZR,Moleküler karakterizasyon,Filogenetik analiz

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