The Effect of Acupuncture on Oxidative Stress in Rats with Femur Fractured

Abstract

The effect of acupuncture on the healing of fractures and oxygen free radicals (ROS) in fractured femur rats has been studied. In this study, 54 female Wistar albino rats (250-300 g in weight) were randomly divided into 10 groups including, control, sacrificed on days 7, 14 and 21 of acupuncture, sacrificed on days 7, 14 and 21 of fracture, and sacrificed on days 7, 14 and 21 of fracture+acupuncture application. The rats in the control group received no treatment.  The 0.5 mm thick kirschner wire was advanced intramedullary as retrograde from the fracture line of the proximal part of the bone and fracture detection was performed. After fracture formation, acupuncture (50 Hz, 20 qA, 25 minutes) was applied to the rats. This treatment was applied for 7, 14 and 21 days as 4 sessions per week. The rats were sacrificed 7, 14 and 21 days after fracture formation and plasma malondialdehyde (MDA), whole blood glutathione (GSH) levels, erythrocyte catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G6PD) activities were measured. Plasma MDA and GSH levels were statistically significant in all fracture groups compared to the control group (p<0.05). It was observed statistically significant reduction in the fracture+acupuncture groups according to the fracture group (p<0.05). While blood CAT activities in 7 day of fracture was observed statistically significant no change compared to the control group, It showed a statistically significant reduction in 14 and 21 days of fracture compared to the control group. There was a statistically significant decrease in blood SOD, GSH-Px and G6PD activities compared to the control group in fracture groups. CAT, SOD, GSH-Px and G6PD activities showed a significant increase in the fracture+acupuncture groups compared to the fracture group (p<0.05). As a result, the application of acupuncture can positively influence the healing process of the fracture by suppressing the harmful effects of ROS and regulating the activities of antioxidant enzymes.

Keywords

• Acupuncture,Antioxidant Enzyme,Glutathione,Fracture Healing,Malondialdehyde

Femur Kırığı Oluşturulmuş Ratlarda Oksidatif Stres Üzerine Akupunkturun Etkisi

Abstract

Femur kırığı oluşturulan ratlarda kırık iyileşmesi ve serbest oksijen radikalleri (SOR) üzerine akupunkturun etkisi araştırıldı. Çalışmada; 54 adet dişi Wistar albino rat (250-300 g ağırlığında), kontrol, 7, 14 ve 21. gün süreyle akupunktur uygulanan gruplar, kırık oluşumunun 7, 14 ve 21. günlerinde sakrifiye edilen gruplar ve 7, 14 ve 21 gün boyunca kırık+akupunktur uygulanan gruplar olmak üzere rastgele 10 gruba ayrıldı. Kontrol grubu ratlara tedavi uygulanmadı. Ratların femurlarında gigli testeresi ile transvers bir kırık hattı oluşturuldu. Kirschner teli kemiğin proksimal parçasının kırık hattından retrograd olarak intramedüller ilerletildi ve kırık tespiti yapıldı. Kırık oluşumunun ardından ratlara akupunktur (50 Hz, 20 qA, 25 dakika) uygulaması yapıldı. Bu tedavi haftada 4 seans olacak şeklinde 7, 14 ve 21 gün süre ile uygulandı. Ratlar kırık oluşturulmasından 7, 14 ve 21 gün sonra sakrifiye edilerek plazma malondialdehit (MDA), tam kan glutatyon (GSH) seviyeleri, eritrosit katalaz (KAT), süperoksit dismütaz (SOD),  glutatyon peroksidaz (GSH-Px) ve glukoz-6-fosfat dehidrogenaz (G6PD) aktiviteleri ölçüldü. Plazma MDA ve kan GSH düzeylerinde tüm kırık gruplarında kontrol grubuna göre istatistiksel olarak önemli bir artma saptandı (p<0,05). Kırık+akupunktur gruplarında kırık gruplarına göre istatistiksel olarak önemli ölçüde azalma gözlendi (p<0,05). Kan KAT aktivitelerinde 7. gün kesilen kırık grubunda kontrol grubuna göre istatistiksel olarak önemli bir değişiklik gözlenmezken, 14 ve 21. günlerde sakrifiye edilen kırık gruplarında kontrol grubuna göre önemli bir azalma saptandı. Kan SOD, GSH-Px ve G6PD aktiviteleri kırık gruplarında kontrol grubuna göre istatistiksel olarak önemli bir azalma saptandı (p<0,05). KAT, SOD, GSH-Px ve G6PD aktivitelerinde kırık+akupunktur gruplarında kırık grubuna göre önemli bir artma gözlendi (p<0,05). Sonuç olarak akupunktur uygulamasının SOR’nin zararlı etkilerini baskılayarak ve antioksidan enzim aktivitelerini düzenleyerek kırık iyileşme sürecini olumlu yönde etkilediği söylenebilir. 

Keywords

• Akupunktur,Antioksidan Enzim,Glutatyon,Kırık İyileşmesi,Malondialdehid

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