Sıçanlarda Silimarinin Beyin Hasarı Üzerine Koruyucu Etkisi

Abstract

Bu çalışmada silimarin’in sıçanlarda histolojik ve biyokimyasal parametrelerle valproik asite bağlı beyin hasarı üzerindeki olası koruyucu etkilerini değerlendirdik. Deney 21 Sprague Dawley erkek sıçan ile yapıldı. Sıçanlar üç gruba ayrıldı: grup 1 kontrol, grup 2 valproik asit ve grup 3 valproik asit + silimarin. Gruplara kontrol grubu dışında 14 gün boyunca 500 mg/kg/gün valproik asit ve 14 gün boyunca 100 mg/kg silimarin verildi. Valproik asit ile artmış serum glikoz, kolesterol, trigliserit ve kreatin kinaz BB seviyeleri, silimarin uygulaması ile tersine çevrildi. Ayrıca, valproik asitle beyin dokusunda artmış malondialdehit ve azalmış glutatyon düzeyleri, silimarin ile anlamlı önemli ölçüde baskılanmıştır. Valproik asit + silimarin grubunda oksidatif stres azaldı, antioksidan aktivite arttı ve valproik asit grubuna göre histopatolojik değişiklikler azaldı. Valproik asidin neden olduğu beyin hasarı, silimarin’in antioksidatif ve anti-apoptotik etkileri nedeniyle silimarin tedavisi ile azaltıldı. Silimarin, valproik asite bağlı beyin hasarının şiddetini azaltmak için yararlı olabilir.

Keywords

• Beyin hasarı
• Sıçan
• Silimarin
• Valproik asit

The Treatment Effect of Silymarin on Brain Damage in Rats

Abstract

Bu çalışmada, silymarinin sıçanlarda histolojik ve biyokimyasal parametreler kullanarak valproik aside bağlı beyin hasarı üzerindeki olası koruyucu etkilerini araştırmayı amaçladık. Deney 21 Sprague Dawley erkek sıçan ile yapıldı. Sıçanlar üç gruba ayrıldı: grup 1; kontrol, grup 2; valproik asit, grup 3; valproik asit + silimarin. Gruplara kontrol grubu hariç 14 gün boyunca 500 mg / kg / gün valproik asit ve 14 gün boyunca 100 mg / kg sililmarin verildi. Valproik asit ile artan glukoz, kolesterol, trigliserit ve kreatin kinaz BB seviyeleri silimarin tedavisi ile tersine çevrilmiştir (p <0.05). Ayrıca, beyin dokusunda valproik asit ile artan malondialdehit ve azalan glutatyon seviyesi, silimarin tarafından önemli ölçüde baskılanmıştır (p <0.05). Histolojik olarak, valproik asit + silimarin grubunda beyin hasarı anlamlı derecede düşük olarak tespit edildi. Valproik asit + silimarin grubunda histopatolojik bulgular valproik asit grubuna göre anlamlı olarak azaldı (p < 0.05). Bu çalışmada silimarinin valproik aside bağlı beyin hasarı üzerinde iyileştirici etkisi olduğu görülmüştür. Bu anlamda çalışmamızın silimarin ve valproik asit ile çalışılacak diğer çalışmalar için faydalı olacağına inanıyoruz.

Keywords

• Brain damage
• Rat
• Silymarin
• Valproic acid

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