Van İlinde Koyunlarda Fasciola Hepatica Prevalansının Koproantijen Elısa Ve Dışkı Muayene Yöntemleri İle Karşılaştırmalı Araştırılması

Year 2020, , 99 - 104, 03.07.2020

Suzan Erol Esim , Nalan Özdal , Bekir Oğuz

https://doi.org/10.36483/vanvetj.662288

Abstract

Bu çalışma, Van ilinde koyunlarda fasciolosisin yayılışını koproantijen-ELISA ve sedimentasyon-çinko sülfat flotasyon yöntemleri ile tespit etmek amacıyla yapılmıştır. Toplam 141 koyundan alınan dışkı örnekleri sedimentasyon–çinko sülfat flotasyon metoduyla Fasciola hepatica yumurtaları yönünden muayene edilmiştir. Pozitif örneklerde gram dışkıdaki yumurta sayısı (EPG) modifiye McMaster sedimentasyon yöntemi ile belirlenmiştir. Dışkı örneklerinde F. hepatica antijenlerinin varlığı koproantijen-ELISA testi ile araştırılmıştır. Fasciolosisin koproantijen-ELISA, dışkı muayenesine ve totale göre prevalansı sırası ile %19.85, %11.34 ve %20.5 belirlenmiştir. Enfekte hayvanlarda EPG değeri 91,6 ± 64.1 bulunmuştur. Fasciolosisin prevalansı 4-5 yaş grubunda (%30) 2-3 yaş grubuna (%13.5) göre daha yüksek belirlenmiş ve bu farklılık istatistiksel olarak önemli bulunmuştur (p<0.05). Cinsiyete göre prevalansın dişilerde ve erkeklerde sırasıyla %20.6 ve %20 olduğu belirlenmiştir (p>0.05). Dışkı muayenesinde, koyunların Dicrocoelium dentriticum (%17.7), Trichuris ovis (%12), Oestertagia spp. (%12), Tricostrongylus spp. (%9.9), Nematodirus spp. (%5.7) ve Paramphistomum spp. (%2.8) ile de enfekte olduğu görülmüştür. Bu çalışma Van ilinde yetiştirilen koyunlarda fasciolosisin koproantijen-ELISA tekniği ile araştırıldığı ilk çalışmadır. Fasciolosis prevalansının uygulanan teşhis metodlarına göre değişebileceği ve sonuçların enfeksiyonun farklı aşamalarını yansıttığı için dikkatli yorumlanması gerektiği düşünülmüştür.

Keywords

Koproantijen-ELISA, Fasciolosis, Prevalans, Koyun, Van

Supporting Institution

Bu çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2015-SBE-YL023 no’lu proje ile desteklenmiştir.

Project Number

2015-SBE-YL023

Thanks

Yazarlar, aynı başlıklı yüksek lisans tezinden özetlenen bu çalışmaya maddi desteklerinden dolayı Van Yüzüncü Yıl Üniversitesi BAP koordinasyon birimine (Proje No: 2015-SBE-YL023) teşekkür ederler.

Comparative Investigation of Fasciola hepatica Prevalence by Coproantigen-Elisa and Stool Examination Methods in Sheep in Van Province

Abstract

This study was carried out to determine the prevalence of fasciolosis in sheep in Van province by coproantigen-ELISA and sedimentation-zinc sulphate flotation technique. Fecal samples collected from 141 sheep were examined by sedimentation-zinc sulphate flotation technique. Modified McMaster sedimentation technique was applied to the egg positive samples to determine the egg per gram feces (EPG) values. Fasciola hepatica coproantigens in fecal samples were investigated by the ELISA technique. The coproantigen-ELISA, coprological, and total prevalence of fasciolosis were determined as 19.85%, 11.34% and 20.5%, respectively. The EPG value in infected sheep was 91,6 ± 64.1. The prevalence of fasciolosis infection was observed higher in 4-5 age group (30%) than 2-3 age group (13.5%), the differences between age groups were found significant (p<0.05). The prevalence of fasciolosis in female and male sheep was found as 20.6% and 20%, respectively (p>0.05). In the fecal examination, sheep were also found to be infected with Dicrocoelium dentriticum (17.7%), Trichuris ovis (12%), Oestertagia spp. (12%), Tricostrongylus spp. (9.9%), Nematodirus spp. (5.7%) and Paramphistomum spp. (2.8%). This study was the first investigation of ovine fasciolosis by coproantigen ELISA technique in Van province. It has been thought that the prevalence of fasciolosis may vary according to the diagnostic methods applied and should be interpreted with caution since the results reflect different stages of the infection.

Keywords

Coproantigen-ELISA, Fasciolosis, Prevalence, Sheep, Van

Project Number

2015-SBE-YL023

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