Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Abstract

Bu çalışmanın amacı, kronik kadmiyuma maruz kalan ratlarda oral melatonin uygulamasının, oksidatif stres ve bağırsak mikroflorasında bulunan bazı mikroorganizmalar üzerine etkilerini araştırmaktır. Sağlıklı 32 yetişkin erkek albino Wistar rat, kontrol (C; n=8), kadmiyum (Cd; n=8), melatonin (Mlt, n=8), kadmiyum + melatonin (Cd + Mlt; n=8) olarak rastgele dört gruba ayrılmıştır. Ratlara oral yolla Mlt (100 mg/kg), (Cd ve Cd + Mlt) haftada 5 kez (Mlt ve Cd + Mlt) ve CdCl2 (2 mg/gün) 3 kez olmak üzere 4 hafta boyunca uygulanmıştır. Uygulamadan sonra serum total antioksidan seviyeleri (TAS), total oksidan (TOS) düzeyleri ile plazma ALT, AST, GGT, T.pro, Alb ve BUN değerleri ölçülmüştür. Ayrıca ince ve kalın bağırsak içerikleri ayrı ayrı aseptik olarak alınarak bağırsak florasında Enterobacteriaceae, Lactococcus spp. ve Lactobacillus spp. sayımları yapılmıştır. Bulgu olarak, serum TOS düzeyleri Cd grubunda diğer gruplara göre daha yüksek olarak tanımlanmıştır (P <0,05). Cd grubunda ince bağırsak florasında Lactococcus spp. sayısı C grubuna göre 0,63 log azalırken, Cd + Mlt grubunda 1,15 log artmıştır. Sonuç olarak, Cd + Mlt şelatının Enterobacteriaceae üremesini baskılanması, Lactobacillus spp./Lactococcus spp. oranını düzenlemesi ve Cd’a bağlı gelişen oksidatif stresi azaltması nedeni ile intestinal mikroflora için faydalı olabilir.

Keywords

• Kadmiyum
• Melatonin
• Mikroflora
• Oksidatif Stres
• Rat

Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium

Abstract

The aim of the present study was to evaluate the effects of oral melatonin treatment on oxidative stress and intestinal microflora in rats exposed to chronic cadmium. Healthy 32 adult male albino Wistar rats were randomly divided into four groups as control (C; n=8), cadmium (Cd; n=8), melatonin (Mlt, n=8), cadmium + melatonin (Cd + Mlt; n=8). Mlt (100 mg/kg) was orally administered 5 times (Mlt and Cd + Mlt), and CdCl2 (2 mg/day) 3 times a week for 4 weeks to rats (Cd and Cd + Mlt). After the treatments, serum total antioxidant status (TAS), total oxidant (TOS) levels as well as plasma ALT, AST, GGT, T.pro, Alb and BUN values were measured. Intestinal contents were aseptically collected, and Enterobacteriaceae, Lactococcus spp. and Lactobacillus spp. counts were performed. As a result, serum TOS levels were defined higher in Cd group than other groups (P <0.05). Lactococcus spp. counts decreased as 0.63 log in Cd group compared to C in small intestine, however it increased as 1.15 log in Cd + Mlt group. In conclusion, Cd + Mlt chelate was found benefical for intestinal microflora due to suppressed the Enterobacteriaceae growth, however ameliorated the Cd induced oxidative stress and Lactobacillus spp./Lactococcus spp. rates in the different part of the intestine.

Keywords

• cadmium
• melatonin
• microflora
• oxidative stress

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