In Vitro Ortamda Kalsiyum Klorürün Osteoblast Benzeri Hücreler Üzerine Etkisi
Year 2021,
, 92 - 97, 26.11.2021
Nasreldin Hassan Ahmed
,
Nedžad Gradaščević
,
Muhamed Katica
Abstract
Bu çalışmada in vitro ortamda kalsiyum klorürün osteoblast benzeri hücrelerin proliferasyonu üzerine cinsiyet bağımlı olarak etkisi olup olmadğının belirlenmesi ve osteoblast benzeri hücrelerin proliferasyonlarını etkileyen en uygun konsantrasyonun belirlenmesi amaçlanmıştır. Her iki cinsiyetten 90- 95 günlük ratların kemik ilikleri çalışmanın biyolojik materyalini oluşturmuştur. Altı farklı kalsiyum klorür konsantrasyonu test edildi ve 24 ve 48 saat sonra osteoblast benzeri hücrelerin sayısal değerleri tespit edildi. 24 saat sonunda erkekler ve dişiler arasındaki ortalama değerlerin test sonuçları, 0.25 mM ve 1.8 mM kalsiyum klorür konsantrasyonları istatistiki olarak anlamlı derecede farklılık göstermiştir(P<0.05). 48 saat sonraki sonuçlar, çoğu CaCl2 konsantrasyonunda önemli farklılıklar olmadığını gösterdi. Erkek osteoblast benzeri hücrelerin, dişi osteoblast benzeri hücrelere kıyasla farklı kalsiyum klorür konsantrasyonları için daha yüksek bir afinite gösterdiği belirlendi. 0.25 mM'lik kalsiyum klorür konsantrasyonu osteoblast benzeri hücrenin proliferasyonunu en olumlu şekilde etkileyen konsantrasyon olarak belirlenmiştir. Bu konsantrasyonda proliferasyon kontrol değerine oranla %26.6 daha fazla olduğu belirlenmiştir.
References
- Bermudez-Reyes B, Del Refugio Lara-Banda M et al. (2018). Effect on growth and osteoblast mineralization of hydroxyapatite-zirconia (HAZrO2) obtained by a new low temperature system. Biomed Mater, 13,1-9.
- Brini M, Ottolini D, Cali T et al. (2013). Calcium in health and disease. Met Ions Life Sci, 13, 81–137.
- Clarke B (2008). Normal bone anatomy and physiology. Clin J Am Soc Nephrol, 3, 131-139.
- Clinckspoor I, Verlinden L, Mathieu C et al. (2013). Vitamin D in thyroid tumorigenesis and development. Prog Histochem Cytochem, 48 (2), 65–98.
- Ćupić V, Kataranovski D, Popović N et al. (1999). Osnovi biologije, kliničke patologije i terapije nedomestifikovanih kućnih ljubimaca. GIK „Ljubostina, Trstenik; Beograd, Srbija.
- Çiçek M, Tumer MK (2018). Yeni Bir Hemostatik Ajan Olan Mecsina Hemostopper® ‘ın Farklı Testlerle Sitotoksisitesinin Değerlendirilip, Kalvarial Osteoblast Proliferasyonuna Etkisinin Araştırılması. Dicle Med J, 45 (3), 291-296.
- D’Amelio P, Rossi P, Isaia G et al. (2008). Bone mineral density and singh index predict bone mechanical properties of human femur. Connect Tissue Res, 49, 99-104.
- Davis JM (1996). Basic cell culture – a practical approach. I Edition, Oxford University Press; England.
- Gila A, Plaza-Diaza J, Mesaa DM (2018). Vitamin D: Classic and Novel Actions. Ann Nutr Metab, 72 (2), 87–95.
- Heino TJ, Hentunen TA, Väänänen HK (2004). Conditioned medium from osteocytes stimulates the proliferation of bone marrow mesenchymal stem cells and their differentiation into osteoblasts. Exp Cell Res, 294 (2), 458-468.
- Jochems C, Islander U, Erlandsson M et al. (2010). Role of endogenous and exogenous female sex hormones in arthritis and osteoporosis development in b10.Q-ncf1*/* mice with collagen-induced chronic arthritis. BMC Musculoskelet Disord, 11, 284.
- Katica M (2007). Effects of vitamin D3 and calcium on the establishment of primary osteoblast culture. Master’s thesis. University of Sarajevo, Faculty of Natural Sciences and Mathematics, Sarajevo/Bosnia and Herzegovina.
- Katica M, Gradascevic N (2017). Hematologic profile of laboratory rats fed with bakery products. I J Res –Granthaalaya, 5 (5), 221-231.
- Katica M, Tepeköy F (2020). The effect of Calcitriol 1,25 (OH)2 - D3 on osteoblast-like cell proliferation during in vitro cultivation. MAE Vet Fak Derg, 5 (1), 11-17.
- Lei Q, Lin D, Huang WX et al. (2018). Effects of calcium ion on the migration and osteogenic differentiation of human osteoblasts. Hua Xi Kou Qiang Yi Xue Za Zhi, 36 (6), 602-608.
- Maniatopoulos C, Sodek J, Melcher AH (1988). Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res, 254 (2), 317-330.
- Marie PJ, Kassem M (2011). Osteoblasts in osteoporosis: past, emerging, and future anabolic targets. Eur J Endocrinol, 165 (1), 1–10.
- Modi PK, Prabhu A, Bhandary YP (2019). Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. PLoS One, 14 (9), 1-15.
- Nakamura S, Matsumoto TT, Sasaki JI et al. (2010). Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts. Tissue Eng Part A, 16 (8), 2467-2473.
- Tosi LL, Boyan BD, Boskey AL (2005). Does sex matter in musculoskeletal health? The influence of sex and gender on musculoskeletal health. J Bone Joint Surg Am, 87, 1631-1647.
- Trailokya A, Srivastava A, Bhole M, Zalte N (2017). Calcium and Calcium Salts. J Assoc Physicians India, 65 (2), 100–103.
- Wlodarski K (1990). Propertietis and Origin of Osteoblasts. Clin Orthopaed Related Res, 252, 276-293.
- Živančević–Simonović S (2006). Opšta patološka fiziologija. Univerzitet u Kragujevcu, Medicinski fakulet Press; Srbija.
Influence of Calcium Chloride on Osteoblast Like Cells of Both Sexes in Rats in In Vitro Conditions
Year 2021,
, 92 - 97, 26.11.2021
Nasreldin Hassan Ahmed
,
Nedžad Gradaščević
,
Muhamed Katica
Abstract
The aim of the study was to determine whether calcium chloride affects the proliferation of osteoblast like cells in a sex-dependent manner, as well as to determine the most effective concentration on proliferation of osteoblast like cells, in in vitro conditions. Bone marrow was used as biological material from young adult rats, both sexes, aged 90-95 days. Six different concentrations of calcium chloride were tested, determining the numerical representation of osteoblast like cells after 24 and 48 hours. Test results of mean values between males and females after 24 hours, indicate significant differences with a probability of P<0.05 at calcium chloride concentrations of: 0.25 mM and 1.8 mM. Results after 48 hours showed that there were no significant differences at most CaCl2 concentrations. It was found that male osteoblast like cells show a higher affinity for different calcium chloride concentrations when compared to the female osteoblast like cells. The calcium chloride concentration of 0.25 mM affected the proliferation of osteoblast like cells most favorably, which is 26.6% higher than the control values.
References
- Bermudez-Reyes B, Del Refugio Lara-Banda M et al. (2018). Effect on growth and osteoblast mineralization of hydroxyapatite-zirconia (HAZrO2) obtained by a new low temperature system. Biomed Mater, 13,1-9.
- Brini M, Ottolini D, Cali T et al. (2013). Calcium in health and disease. Met Ions Life Sci, 13, 81–137.
- Clarke B (2008). Normal bone anatomy and physiology. Clin J Am Soc Nephrol, 3, 131-139.
- Clinckspoor I, Verlinden L, Mathieu C et al. (2013). Vitamin D in thyroid tumorigenesis and development. Prog Histochem Cytochem, 48 (2), 65–98.
- Ćupić V, Kataranovski D, Popović N et al. (1999). Osnovi biologije, kliničke patologije i terapije nedomestifikovanih kućnih ljubimaca. GIK „Ljubostina, Trstenik; Beograd, Srbija.
- Çiçek M, Tumer MK (2018). Yeni Bir Hemostatik Ajan Olan Mecsina Hemostopper® ‘ın Farklı Testlerle Sitotoksisitesinin Değerlendirilip, Kalvarial Osteoblast Proliferasyonuna Etkisinin Araştırılması. Dicle Med J, 45 (3), 291-296.
- D’Amelio P, Rossi P, Isaia G et al. (2008). Bone mineral density and singh index predict bone mechanical properties of human femur. Connect Tissue Res, 49, 99-104.
- Davis JM (1996). Basic cell culture – a practical approach. I Edition, Oxford University Press; England.
- Gila A, Plaza-Diaza J, Mesaa DM (2018). Vitamin D: Classic and Novel Actions. Ann Nutr Metab, 72 (2), 87–95.
- Heino TJ, Hentunen TA, Väänänen HK (2004). Conditioned medium from osteocytes stimulates the proliferation of bone marrow mesenchymal stem cells and their differentiation into osteoblasts. Exp Cell Res, 294 (2), 458-468.
- Jochems C, Islander U, Erlandsson M et al. (2010). Role of endogenous and exogenous female sex hormones in arthritis and osteoporosis development in b10.Q-ncf1*/* mice with collagen-induced chronic arthritis. BMC Musculoskelet Disord, 11, 284.
- Katica M (2007). Effects of vitamin D3 and calcium on the establishment of primary osteoblast culture. Master’s thesis. University of Sarajevo, Faculty of Natural Sciences and Mathematics, Sarajevo/Bosnia and Herzegovina.
- Katica M, Gradascevic N (2017). Hematologic profile of laboratory rats fed with bakery products. I J Res –Granthaalaya, 5 (5), 221-231.
- Katica M, Tepeköy F (2020). The effect of Calcitriol 1,25 (OH)2 - D3 on osteoblast-like cell proliferation during in vitro cultivation. MAE Vet Fak Derg, 5 (1), 11-17.
- Lei Q, Lin D, Huang WX et al. (2018). Effects of calcium ion on the migration and osteogenic differentiation of human osteoblasts. Hua Xi Kou Qiang Yi Xue Za Zhi, 36 (6), 602-608.
- Maniatopoulos C, Sodek J, Melcher AH (1988). Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res, 254 (2), 317-330.
- Marie PJ, Kassem M (2011). Osteoblasts in osteoporosis: past, emerging, and future anabolic targets. Eur J Endocrinol, 165 (1), 1–10.
- Modi PK, Prabhu A, Bhandary YP (2019). Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. PLoS One, 14 (9), 1-15.
- Nakamura S, Matsumoto TT, Sasaki JI et al. (2010). Effect of Calcium Ion Concentrations on Osteogenic Differentiation and Hematopoietic Stem Cell Niche-Related Protein Expression in Osteoblasts. Tissue Eng Part A, 16 (8), 2467-2473.
- Tosi LL, Boyan BD, Boskey AL (2005). Does sex matter in musculoskeletal health? The influence of sex and gender on musculoskeletal health. J Bone Joint Surg Am, 87, 1631-1647.
- Trailokya A, Srivastava A, Bhole M, Zalte N (2017). Calcium and Calcium Salts. J Assoc Physicians India, 65 (2), 100–103.
- Wlodarski K (1990). Propertietis and Origin of Osteoblasts. Clin Orthopaed Related Res, 252, 276-293.
- Živančević–Simonović S (2006). Opšta patološka fiziologija. Univerzitet u Kragujevcu, Medicinski fakulet Press; Srbija.