Effects of Marbofloxacin, Diclofenac Sodium And Methylprednisolone on Serum Biochemical Values in Endotoxemia-Shaped Rats

Abstract

Escherichia coli-derived lipopolysaccharide (LPS) has been used extensively as a model for septic shock and sepsis. In our study, it was aimed to induce experimental endotoxemia in rats by using LPS and the effects of marbofloxacin, diclofenac sodium and methylprednisolone on alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), blood urea nitrogen (BUN), creatinine, which are indirect indicators of organ insufficiency, were assessed. 186 rats were divided into 5 groups. Blood samples were obtained from control group at 0 h. To shape endotoxemia, LPS was administered at a dose of 4 mg/rat via intraperitonally (IP). Marbofloxacin (100 mg/kg, IP), diclofenac sodium (10 mg/kg, IP) and metylprednisolone (10 mg/kg, IP) were administered at dosage for the treatment of developing endotoxemia. Blood samples were collected under thiopental anesthesia at 1, 2, 4, 8, 12 and 24 h. They were analyzed biochemical levels. In the study, it was determined that serum ALP, ALT, AST, GGT,BUN and creatinine levels increased with LPS application (P<0.05) and the expected effect in sepsis was formed. As a result, LPS administration caused sepsis and it had several effects observed in the kidney and liver. It was concluded that in the treatment of sepsis, drugs other than methylprednisolone cannot be used alone, but combined application can be preferred.

Keywords

• Diclofenac sodium
• endotoxemia
• methylprednisolone.

Project Number

2010-SBE-D174

Endotoksemi Şekillendirilmiş Ratlarda Marbofloksasin, Diklofenak Sodyum ve Metilprednizolonun Serum Biyokimyasal Değerler Üzerine Etkisi

Abstract

Escherichia coli'den türetilen lipopolisakkarit (LPS), sepsis ve septik şok için bir model olarak yaygın olarak kullanılmıştır. Çalışmamızda LPS ile deneysel endotoksemi oluşturulan ratlarda, marbofloksasin, diklofenak sodyum, metilprednizolon kullanılarak, bu ilaçların organ yetmezliğinin indirekt belirteçleri olan alkalen fosfotaz (ALP), alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), gama glutamil transferaz (GGT), kan üre azot (BUN), kreatinin değerleri üzerine olan etkilerinin değerlendirilmesi amaçlanmıştır. Çalışma için gerekli 186 adet rat, 5 gruba ayrıldı. Kontrol grubundan 0. saatte kan örnekleri alındı. Ratlarda endotoksemi oluşturmak amacı ile intraperitoneal (IP) yolla LPS (4mg/rat) uygulandı. Gelişen endotoksemiyi tedavi etmek için marbofloksasin IP yolla 100 mg/kg, diklofenak sodyum IP yolla 10 mg/kg, metilprednizolon IP yolla 10 mg/kg dozunda uygulandı. Daha sonra 1, 2, 4, 8, 12 ve 24. saatlerde tiyopental anestezisi altında kan örnekleri alınarak biyokimyasal değerler ölçüldü. Çalışmada serum ALP, ALT, AST, GGT, BUN ve kreatinin düzeylerinin LPS uygulaması ile arttığı (P<0.05) ve sepsiste beklenilen etkinin şekillendiği tespit edildi. Sepsis tedavisinde, metilprednizolon dışında diğer ilaçların tek başlarına kullanılamayacağı ancak kombine uygulamanın tercih edilebileceği sonucuna ulaşıldı.

Keywords

• Diklofenak sodyum
• endotoksemi
• metilprednizolon.

Supporting Institution

Bu araştırma Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Project Number

2010-SBE-D174

Thanks

Bu araştırma Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından 2010-SBE-D174 no’lu proje ile desteklenmiştir

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