Kars ilindeki koyunlarda Bluetongue Virus ve Rift Valley Fever Virus infeksiyonlarının serolojik araştırılması

Year 2015, Volume 26, Issue 3, 119 - 122, 01.12.2015

Volkan YILMAZ Yakup YILDIRIM Nüvit COŞKUN

Abstract

Bu çalışmada Kuzeydoğu Anadolu Bölgesinde yer alan Kars ilinde küçük aile işletmelerindeki koyunlarda Bluetongue Virus (BTV) ve Rift Valley Fever Virus (RVFV) enfeksiyonları serolojik olarak araştırıldı. Bu amaçla, yerel ırka ait 460 adet koyundan rastgele toplanan kan serumları BTV ve RVFV spesifik antikor varlığı yönünden sırasıyla yarışmalı enzyme linked immunosorbent assay (c-ELISA) ve bloklama ELISA (b-ELISA) testi ile analiz edildi. Koyunlarda BTV için %10.65 seropozitiflik saptanmasına karşın, RVFV yönünden antikor cevabı tespit edilemedi. Elde edilen veriler, kiiçük aile işletmelerinde enfeksiyonun yayılma gösterdiğini ortaya koydu. Bu bilgilerden hareketle bluetongue virus enfeksiyonunun kontrolüne yönelik önerilerde bulunuldu. Ayrıca bu bölgede RVFV varlığı tespit edilmedi. Bu çalışma Kars ilindeki koyunlarda BTV ve RVFV enfeksiyonlarının serolojik olarak araştırıldığı ilk çalışmadır

Keywords

b-ELISA, BTV, c-ELISA, RVFV, Kars

Serological Investigation of Bluetongue Virus and Rift Valley Fever Virus Infections in Sheep in Kars Province of Turkey

Abstract

In this study we surveyed Bluetongue Virus (BTV) and Rift Valley Fever Virus (RVFV) infections as serologically in sheep from private small scale production units of less than twenty sheep per unit, in Kars province of the Northeast Anatolia Region Turkey. For this purpose, blood samples randomly collected from 460 local sheep were analyzed for the presence of specific antibodies for BTV using a competative enzymelinked immunosorbent assay (c-ELISA) and RVFV using blocking ELISA (b-ELISA), respectively. A total of 49 (10.65%) serum samples were found positive against BTV. None of the sheep were positive for antibodies to RVFV. On the basis of results it is suggested that infection was spreading in small private f1ocks. Furthermore, recommendation to the control of bluetongue infection were presented. In addition, the RVFV infection is not present in this region. This study is the first serological study on BTV and RVFV in sheep in Kars province of Turkey

Keywords

b-ELISA, BTV, c-ELISA, RVFV, Kars

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