The Effect of Rosmarinus officinalis L. Aqueous Extract on Gastric Acid Secretion in Isolated Rats Stomach

Year 2017, Volume: 28 Issue: 2, 95 - 99, 20.08.2017

Volkan Gelen Fikret Çelebi Emin Şengül Ali Çınar

Abstract

In this study, the in vitro effect of Rosmarinus
officinalis L. (Rosemary) aqueous extract on rat gastric acid secretion was
investigated. 24 male Sprague Dawley male rats were used in the study. Rats
were euthanized by cervical dislocation in the presence of ketamine
hydrochloride (Ketalar: 75 mg / kg) and xylazine (Rompun: 15 mg / kg) anesthesia.
The isolated stomachs were left in the isolated organ bath for 1 hour for the
incubation period. At the end of this period, the pH of the mucosal fluid
obtained from the stomachs was evaluated as the basal pH. Histamine was then
applied to the bath environment to induce stomach acid secretion and the pH
values of the stomach contents were measured. Four different doses of Rosemary
extract (150, 300, 600 and 1200 μg / ml) were applied to the bath separately
and the pH of the stomach contents was recorded. In addition, Rosemary
Extract's four separate doses were applied to the individual baths with
Histamine and the pH values of the stomach contents were determined. The
resulting pH values were compared to the basal pH and the ΔPH values were
calculated. In conclusion, the effect of 150 μg / ml dose of Rosemary extract
on gastric acid secretion was not statistically significant and doses of 300,
600 and 1200 μg / ml increased stomach acid secretion statistically
significantly.

Keywords

Rosemary, Histamine, Rat, Gastric acid secretion

Rosmarinus officinalis L. Sulu Ekstraktının İzole Edilmiş Rat Midesinde Mide Asit Salgısı Üzerine Etkisi

Abstract

Bu
çalışmada Rosmarinus officinalis L.
(Rosemary) sulu ekstraktının rat mide asit salgısı üzerine in vitro etkisi araştırıldı. Araştırmada 24 adet Sprague Dawley
cinsi ergin erkek rat kullanıldı. Ratlar ketamine hydrochloride (Ketalar: 75
mg/kg) ve xylazine (Rompun: 15 mg/kg) anestezisi eşliğinde servikal dislokasyon metodu ile ötenazi edilerek mideleri
izole edildi. İzole edilen mideler izole organ banyosunda 1 saat inkubasyon
periyoduna bırakıldılar. Bu periyodun sonunda, midelerden elde edilen mukozal
sıvının pH’sı bazal pH olarak değerlendirildi. Daha sonra banyo ortamına
Histamin uygulanarak mide asit salgısı indüklendi ve mide içeriğinin pH
değerleri ölçüldü. Rosemary ekstraktının dört farklı dozu (150, 300, 600 ve
1200 µg/ml) banyoya ayrı ayrı uygulandı ve mide içeriklerinin pH’sı kaydedildi.
Ayrıca Rosemary ekstraktının dört farklı dozu tek tek banyoya Histaminle ile
birlikte uygulandı ve mide içeriklerinin pH değerleri belirlendi. Elde edilen
pH değerleri, bazal pH ile karşılaştırıldı ve ∆pH değerleri hesaplandı. Sonuç
olarak, Rosemary ekstraktının 150 µg/ml dozunun mide asit salgısı üzerine
etkisinin istatistiksel olarak anlamlı olmadığı ve 300, 600 ve 1200 µg/ml
dozlarının mide asit salgısını istatistiksel olarak anlamlı şekilde artırdığı
belirlendi.

Keywords

Rosemary, Histamin, Rat, Mide Asit Salgısı

References

• Abdul-Hussein M, Freeman J, Castell D (2015). Concomitant administration of a histamine2 receptor antagonist and proton pump inhibitor enhances gastric acid suppression. Pharmacotherapy, 35, 1124–1129. Afonso MS, de O Silva AM, Carvalho EB, Rivelli DP, Barros SB, Rogero MM, Lottenberg AM, Torres RP, Mancini-Filho J (2013). Phenolic compounds from Rosemary (Rosmarinus officinalis L.) attenuate oxidative stress and reduce blood cholesterol concentrations in diet-induced hypercholesterolemic rats. Nutr Metab (Lond), 10(1), 19. Altamirano M (2016). Backdiffusion of H+ during gastric secretion. American Journal of Physiology-Legacy Content, 218, 1-6. Al-Sereiti MR, Abu-Amer KM, Sen P (1999). Pharmacology of Rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials. Indian J Exp Biol, 37, 124-30. Altinier G, Sosa S, Aquino RP, Mencherini T, Della Loggia R, Tubaro A (2007). Characterization of topical antiinflammatory compounds in Rosmarinus officinalis L. J Agric Food Chem, 55(5), 1718-23. Amaral GP, de Carvalho NR, Barcelos RP, Dobrachinski F, Portella Rde L, da Silva MH, Lugokenski TH, Dias GR, da Luz SC, Boligon AA, Athayde ML, Villetti MA, Antunes Soares FA, Fachinetto R (2013). Protective action of ethanolic extract of Rosmarinus officinalis L. in gastric ulcer prevention induced by ethanol in rats. Food Chem Toxicol, 55, 48-55. Amin A, Hamza AA (2005). Hepatoprotective effects of Hibiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci, 77(3), 266-78. Aqel MB (1991). Relaxant effect of the volatile oil of Rosmarinus officinalis on tracheal smooth muscle. J Ethnopharmacol, 33(1-2), 57-62. Argueta A, Cano L, Rodarte M (1994). Atlas of Plants from Mexican traditional medicine. Indigenist National Institute, Mexico. 1786 pp. Bai N, He K, Roller M, Lai CS, Shao X, Pan MH, Ho CT (2010). Flavonoids and phenolic compounds from Rosmarinus officinalis. J Agric Food Chem, 58(9), 5363-7. Bakirel T, Bakirel U, Keleş OU, Ulgen SG, Yardibi H (2008). In vivo assessment of antidiabetic and antioxidant activities of Rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits. J Ethnopharmacol, 116(1), 64-73. Dias PC, Foglio MA, Possenti A, de Carvalho J (2000). Antiulcerogenic activity of crude hydroalcoholic extract of Rosmarinus officinalis L. J Ethnopharmacol, 69(1), 57-62. Engelstoft MS, Lund ML, Grunddal KV (2015). Research resource: a chromogranin A reporter for serotonin and histamine secreting enteroendocrine cells. Mol Endocrinol, 29, 1658–1671. González-Trujano ME, Peña EI, Martínez AL, Moreno J, Guevara-Fefer P, Déciga-Campos M, López-Muñoz FJ (2007). Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different experimental models in rodents. J Ethnopharmacol, 111(3), 476-82. Haloui M, Louedec L, Michel JB, Lyoussi B (2000). Experimental diuretic effects of Rosmarinus officinalis and Centaurium erythraea. J Ethnopharmacol, 71(3), 465-72. Harmankaya S, Vatansever L (2017). The Effect of Essential Oils of Rosemary and Clove on Shelf Life Chicken Meat. Van Vet J, 28 (1), 11-9 Ho CT, Wang M, Wei GJ, Huang TC, Huang MT (2000). Chemistry and antioxidative factors in Rosemary and sage. Biofactor, 13(1-4), 161-6. Jiang H, Wang W, Zhang Y (2015). Cell polarity kinase MST4 cooperates with cAMP-dependent kinase to orchestrate histamine-stimulated acid secretion in gastric parietal cells. J Biol Chem, 47, 28272-28285. Komasaka M, Horie S, Watanabe K, Murayama T (2002). Antisecretory effect of somatostatin on gastric acid via inhibition of histamine release in isolated mouse stomach. Eur J Pharmacol, 452(2), 235-43. Martínez M (1989). Las plantas medicinales de Mexico, 6th ed. Botas, Mexico. 412-488. Mengoni ES, Vichera G, Rigano LA, Rodriguez-Puebla ML, Galliano SR, Cafferata EE, Pivetta OH, Moreno S, Vojnov AA (2011). Suppression of COX-2, IL-1β and TNF-α expression and leukocyte infiltration in inflamed skin by bioactive compounds from Rosmarinus officinalis L. Fitoterapia, 82(3), 414-21. Nadaroglu H, Nazan D, Yasar D (2009). Antioxidant and radical scavenging activities of capsules of caper (Capparis spinosa). Asian Journal of Chemistry, 21, 5123-34. Panula P, Chazot PL, Cowart M (2015). International union of basic and clinical pharmacology. XCVIII. Histamine receptors. Pharmacol Rev, 67, 601-655. Phan J, Benhammou JN, Pisegna JR (2015). Gastric hypersecretory states: investigation and management. Curr Treat Options Gastroenterol, 13(4), 386-397. Ramadan KS, Khalil OA, Danial EN, Alnahdi HS, Ayaz NO (2013). Hypoglycemic and hepatoprotective activity of Rosmarinus officinalis extract in diabetic rats. J Physiol Biochem, 69(4), 779-83. Romo de Vivar (1985). Natural Products from Mexican Flora. Limusa, Mexico. 56 pp. (in Spanish) Sagorchev P, Lukanov J, Beer AM (2010). Investigations into the specific effects of Rosemary oil at the receptor level. Phytomedicine, 17(8-9), 693-7. Sakr SA, Lamfon, HA (2012). Protective effect of rosemary (Rosmarinus officinalis) leaves extract on carbon tetrachloride-induced nephrotoxicity in albino rats. Life Sci J, 9(1), 779-85. Savegnago L, Trevisan M, Alves D, Rocha JBT, Nogueira CW, Zeni G (2006). Antisecretory and antiulcer effects of diphenyl diselenide. Environmental Toxicology and Pharmacology, 21, 86-92. Sotelo-Félix JI, Martinez-Fong D, Muriel P, Santillán RL, Castillo D, Yahuaca P (2002). Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat. J Ethnopharmacol, 81(2), 145-54. Takaki I, Bersani-Amado LE, Vendruscolo A, Sartoretto SM, Diniz SP, Bersani-Amado CA, Cuman RK (2008). Anti-inflammatory and antinociceptive effects of Rosmarinus officinalis L. essential oil in experimental animal models. J Med Food, 11(4), 741-6 Topal A, Çelebi F (2011). Effects of Nigella sativa aqueous extracts on gastric acid secretion in isolated rat stomach. Kafkas Univ Vet Fak Derg, 17,531-6. Ventura-Martínez R, Rivero-Osorno O, Gómez C, González-Trujano ME (2011). Spasmolytic activity of Rosmarinus officinalis L. involves calcium channels in the guinea pig ileum. J Ethnopharmacol, 137(3), 1528-32. Waldum HL, Hauso Ø, Brenna E, Qvigstad G, Fossmark R (2016). Does long-term profound inhibition of gastric acid secretion increase the risk of ECL cell-derived tumors in man? Scand J Gastroenterol, 51(7), 767-773. Waldum HL, Kleveland PM, Fossmark R (2015). Upper gastrointestinal physiology and diseases. Scand J Gastroenterol, 50, 649-656. Wang T, Takikawa Y, Satoh T, Yoshioka Y, Kosaka K, Tatemichi Y, Suzuki K (2011). Carnosic acid prevents obesity and hepatic steatosis in ob/ob mice. Hepatol Res, 41(1), 87-92. Yamamoto J, Yamada K, Naemura A, Yamashita T, Arai R (2005). Testing various herbs for antithrombotic effect. Nutrition, 21(5), 580-7. Yang L, Wei-Ping W, Hong-Ying W, Chi-Hin C (2000). Intragastric administration of heparin enhances gastric ulcer healing through a nitric oxide-dependent mechanism in rats. Eur J of Pharmacol, 399, 205-14.