Serologic and Molecular Investigation of Q Fever on Water Buffalo in Afyon

Year 2016, Volume: 27 Issue: 1, 17 - 19, 20.04.2016

Elçin Günaydın Selçuk Petekkaya

Abstract

The water buffalo were usually grown for milk production and famous by-products, semi-hard cheese and especially cream clotting in Afyon. In this study, it was aimed to determine the risk of Q fever infection among the water buffalos, in Afyon. The collected 92 serum and 92 blood samples of female water buffalos were examined to investigate for the different stages of infection.  A total of 92 serum samples was examined for the presence of Coxiella burnetii(C. burnetii) antibodies against phase I and phase II antigens by indirect ELISA. Eight (8.69 %) out of 92 serum samples were found to be antibody positive. Of the examined blood samples, none of them were found to harbor C. burnetiiDNA.

Keywords

Q Fever, Water buffalo, ELISA, PCR

Afyon İlinde Mandalarda Q Humması’nın Serolojik ve Moleküler Araştırılması

Abstract

Afyon’da manda, süt ve süt yan ürünlerinden peynir, özellikle de kaymak üretimi amacıyla yetiştirilmektedir. Bu çalışmada, Afyon ilinde mandalarda Q humması riskini araştırmak amaçlanmıştır. Dişi mandalardan toplanan 92 serum ve 92 kan örneği, infeksiyonun farklı dönemlerini tespit etmek amacıyla incelendi. Toplam 92 serum örneğinde, Coxiella burnetii(C. burnetii) faz 1 ve faz 2 antijenlerine karşı antikor varlığı indirekt ELISA ile incelendi. Doksaniki örneğin 8’i (%8.69) antikor pozitif bulundu. İncelenen kan örneklerinde C. burnetiiDNA’sına rastlanmadı.

Keywords

Q Humması, Manda, ELISA, PCR

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