Evaluation of Some Sperm Parameters with Contaminated Escherichia coli of Bull Semen

Year 2016, Volume: 27 Issue: 2, 91 - 96, 20.08.2016

Akın Gülyüz Barış Atalay Uslu

Abstract

One of the common reasons disrupting spermatogenesis is infections directly affecting testes. Escherichia coliis the most abundant bacteria found in bovine faeces. This study was performed in order to evaluate changes in motility, dead-alive spermatozoa ratio and fertility in commercially used semen in the veterinary field which are contaminated by E. coli. For this purpose, 40 straws of bull semen were thawed in 37 °C and separated into 4 groups. Each group had 10 straws of thawed semen. Placebo, 10.00 E. coli/ml, 100.00 E. coli/ml and 1.000.000 E. coli /ml were added to 1^st, 2^nd, 3^rdand 4^thgroup, respectively. The initial motility was determined as 80%. Later, motility examination was performed in the first 2 hour with 10 minutes intervals and after the next 1 hour with 15 minutes intervals and finally in every hour. While dead-alive ratio was determined as 8-10% initially, all of the data were recorded in order to support motility from the outset of the study every hour. In group II, the motility was abolished faster than all test groups. The highest increase determined in dead spermatozoon rate is also group II. As a result, the importance of antimicrobial agents used against bacterial contamination and in semen production stages become prominent. Moreover, practitioners should show maximum attention towards asepsis while performing artificial insemination.

Keywords

Semen, E. coli, Contamination, Motility, Fertility

Escherichia coli ile Kontamine Edilen Boğa Spermalarında Bazı Spermatolojik Parametrelerinin Değerlendirilmesi

Abstract

Spermatogenezisi etkileyen en önemli nedenlerden birisi, testisleri direkt etkileyen enfeksiyonlardır. Bu çalışma, sığır dışkısında en fazla bulunan bakteri olan Escherichia coli’nin; ticari olarak satılan ve rutinde Veteriner Hekimler’in suni tohumlamada kullandıkları spermaların, motilite ve ölü-canlı spermatozoon oranı, dolayısı ile fertilitenin, kontaminasyon sonucunu nasıl etkilendiğinin ortaya konulmasını belirlemek amacıyla yapılmıştır. Bu amaçla 40 payet boğa sperması 37 °C’de çözdürülerek 4 gruba ayrılmıştır. Her bir gruba 10 payet olacak şekilde çözdürülen spermaların üzerine 1. Grupta placebo, 2. Grupta 1 ml’de 10.000 E. coli, 3. Grupta 1 ml’de 100.000 E. colive 4. Grupta 1 ml’de 1.000.000 E. coliolacak şekilde bakteriler eklenmiştir. Başlangıç motilitesi %80 olarak belirlenmiş, daha sonra motilite muayenesi ilk 2 saat içerisinde 10’ar dakika aralıklarla, sonraki 1 saatte 15 dakika aralıklarla ve sonrasında her saat başı yapılmıştır. Ölü-canlı oranı başlangıçta %8-10 olarak belirlenirken, motiliteyi desteklemek için çalışma başından itibaren her saat başı yapılarak tüm veriler kaydedilmiştir. Deneme grupları içerisinde motilite en çabuk Grup II’de sonlanmıştır. Ölü spermatozoon oranınındaki en hızlı artış da Grup II’de gerçekleşmiştir. Sonuç olarak bakteriyel kontaminasyonun ve sperma üretim aşamalarında katılan antimikrobiyel maddelerin önemi bir kez daha ortaya çıkmaktadır. Dahası suni tohumlama uygulayıcılarının asepsiye azami dikkat etmelerinin gerekliliği de ortaya çıkmaktadır.

Keywords

Sperma, E. coli, Kontaminasyon, Motilite, Fertilite

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