Tavşanlarda Mistletoe Lektin-I’in Farklı Dozlarının Tümör Nekroz Faktör-α, Nitrik Oksit, Total Antioksidan ve Oksidan Kapasite Düzeylerine Etkisi

Year 2017, Volume: 28 Issue: 1, 41 - 45, 20.04.2017

Ahmet Harmankaya , Ayla Özcan

Abstract

Mistletoe
lektin-I (ML-I)’in, farklı dozlarının tümör nekroz faktör-α (TNF-α), nitrik
oksit (NO), total antioksidan ve oksidan kapasite (TAK, TOK) düzeylerine
etkisinin araştırıldığı çalışmada, her birinde 7 adet tavşan bulunan, birisi
kontrol olmak üzere 4 grup oluşturuldu. Gruplara sırasıyla 0.5, 1 ve 2 ng/kg
ML-I intraperitonal (i.p.) olarak enjekte edildi. Enjeksiyonu takiben 1., 6.,
12. ve 24. saatlerde kan örnekleri alınarak plazmaları elde edildi. 0.5 ng/kg
ML-I verilen grupta TNF-α düzeyinin bütün saatlerde kontrol ve diğer lektin
gruplarına göre yüksek olmakla birlikte 1. saatte en yüksek değerine ulaştığı
(p<0.01) saptandı. 0.5 ve 1 ng/kg lektin dozlarının 1. saatte NO düzeyini
azalttığı (p<0.05), 1 ve 2 ng/kg lektin dozlarının 12. saatte NO düzeyini
arttırdığı (p<0.05), gruplar arasında istatistiksel olarak bir farkın
oluşmadığı gözlendi. 0.5 ng/kg ML-I verilen gruptan alınan numunelerde, 1 saat
sonra elde edilen TAK değerlerinin 6, 12 ve 24. saatlere göre istatistiksel
olarak yüksek (p<0.05) olduğu, TOK düzeylerinde istatistiksel olarak bir
değişim olmadığı gözlemlendi. 0.5 ng/kg dozundaki ML-I’in TNF-α ve NO
düzeylerinde değişiklik meydana getirmesi, 
lektinin akut durumlarda antioksidan özellik gösterebileceği kanaatini
oluşturdu.

Keywords

Mistletoe Lektin-I, TNF-α, Nitrik Oksit, Total Antioksidan ve Oksidan Kapasite

Effect of Different Doses of Mistletoe Lectin-I on the Levels of Tumor Necrosis Factor-α, Nitric Oxide, Total Antioxidant and Oxidant Capacity in Rabbits

Abstract

This
paper aimed to investigate the effect of different doses of mistletoe lectin-I
(ML-I) on the levels of tumor necrosis factor-α (TNF-α), nitric oxide (NO),
total antioxidant and oxidant capacity (TAC, TOC). The rabbits were divided
into 4 groups (Control, Group-I, II and III) each group contained 7
rabbits.  0.5, 1 and 2 ng/kg of ML-I were
administered to treatment groups via intraperitoneal route, respectively.
Plasmas of blood samples obtained in the 1^st, 6^st, 12^th
and 24^th hours following injection were seperated. TNF-α level in
the group treated with ML-I (0.5 ng/kg) was higher than that of control and
other lectin receiving groups at all hours, the highest value of TNF-α was
reached at 1^st hour (p<0.01). 0.5 and 1 ng/kg doses of lectin
decreased NO level at 1^st hour compared to control group
(p<0.05), 1 and 2 ng/kg doses of lectin increased NO levels (p<0.05) at
12^th hour, no statistically significant difference was found between
the groups. In addition, TAC levels in samples
obtained from the group given 0.5 ng/kg ML-I after 1 hour was statistically
higher (p<0.05) than the samples taken at 6^th, 12^th
and 24^th hours. No statistically significant change was found in the
level of TOC among lectin receiving groups. In conclusion, ML-I at 0.5 ng/kg
dose caused alterations in the levels of TNF-α and NO and showed an acute
antioxidant effect.

Keywords

Mistletoe Lectin-I, TNF-α, Nitric Oxide, Total Antioxidant and Oxidant Capacity

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