Malondialdehyde and Total Antioxidant Levels and Hematological Parameters of Beef Cattle with Coccidiosis
Abstract
The present study evaluates the malondialdehyde (MDA) level of serum
lipid peroxidation and total antioxidant capacity in order to determine the
effects of coccidiosis on oxidative stress in beef cattle. It will be
determined in this study whether lipid peroxidation plays a role in the
pathogenesis of the disease. This
study included 10 beef cattle diagnosed with coccidiosis via clinical and stool
examinations (Patient group) and 10 healthy matched animals from the same barn
(Control group). General clinical findings (body temperature, heart and
respiration rates, and rumen movements), hematological parameters (total leukocyte
count, erythrocyte count, hematocrit value and hemoglobin count), and serum MDA
levels and total antioxidant capacities were determined. A significant increase was
observed in the body temperature, heart and respiration rates, total leukocyte
count, and serum MDA levels of the patient group compared to the controls,
while the rumen movements, hematocrit and hemoglobin values, and serum total
antioxidant capacities showed significant reductions (P<0.05). The results of the present research suggest that prophylactic measures
should be taken in animals with coccidiosis, as the defense system might be
infected due to the increase in MDA level. It will be shown whether these
parameters can be taken as references with other parameters in order to make
diagnosis as well as the efficiency of treatment.
References
- Aiello SE, Mays A (1998). The Merck Veterinary Manual. 8th edition, Merck Co, USA. Anderson DE, Rings DM (2009). Current Veterinary Food Animal Practice. 5th edition, Saunders Elsevier, Missouri. Argenzio RA, Rhoads JM (1997). Reactive oxygen metabolites in piglet cryptosporidiosis. Pediatr Res, 41, 521–526. Aumont G, Yvore P, Esnault, A (1986). Experimental coccidiosis in goats. 2. Effects of parasitism on nutritional balances and some blood parameters. Ann Rech Vet, 17, 191-196. Aydın UE, Aslan Ö (2012). Buzağı coccidiosis’inde bazı pıhtılaşma parametrelerinin belirlenmesi. Vet Hekim Der Derg, 83, 2, 1–8. Bangoura B, Daugschies A (2007). Parasitological and clinical parameters of experimental Eimeria zuernii infection in calves and infl uence on weight gain and haemogram. Parasitol Res, 100, 6, 1331–1340. Cam Y, Atasever A, Eraslan G, Kibar M, Atalay Ö, Beyaz L, Inci A, Liman BC (2008). Eimeria stiedae: Experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin. Exp Parasitol, 119, 164–172. Ching S, Ingram D, Hahnel R, Beilby J, Rossi E (2002). Serum levels of micronutrients, antioxidants and total antioxidant status predict risk of breast cancer in a case control study. J Nutr, 132, 303–306. Daugschies A, Najdrowski M (2005). Eimeriosis in cattle: Current understanding. J Vet Med B Infect Dis Vet Public Health, 52, 417-427. Dede S, Deger Y, Alkan M, Cemek M (2002). Oxidation products of nitric oxide and the concentration of antioxidant vitamins in parasitized goats. Acta Vet Brno, 71, 1–5. Dede S, Deger Y, Deger S, Alkan M (2000). Determination of the status of lipid peroxidation and antioxidants in sheep infected with certain endoparasites (Fasciola spp., Trichostronglidae spp., Eimeria spp.). Acta Parasit Turc, 24, 190–193. Dharmendra Kumar BV, Hafeez MD (1999). Efficacy of monensin and amprolium against subclinical coccidiosis in lambs. Indian Vet J, 1999; 76, 965–967. Dincer S (2001). Türkiye parazitoloji derneği. Meta Press, İzmir. Eraslan G, Cam Y, Eren M, Liman BC (2004). Changes in malondialdehyde level and catalase activity and effect of toltrazuril on these parameters in chicks infected with Eimeria tenella. B Vet I Pulawy, 48, 251–255. Erel O (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem, 37: 112–119. Erel O, Vural H, Aksoy N, Aslan G, Ulukanligil M (2001). Oxidative stress of platelets and thrombocytopenia in patients with vivax malaria. Clin Biochem, 34, 341–344. Georgi JR, Theodories VJ (1980). Parasitology for Veterinarians. 4th edition, W.B. Saunders Company, London. Georgieva NV, Koinarski V, Gadjeva V (2006). Antioxidant status during the course of Eimeria tenella infection in broiler chickens. Vet J, 172, 488–492.
Koksidiyozisli Besi Danalarında Malondialdehid ve Total Antioksidan Düzeyleri ile Hematolojik Parametreler
Abstract
Bu
çalışmada, koksidiyozisin oksidatif stres üzerine etkilerini araştırmak için
serum lipid
peroksidasyon ürünü olan malondialdehid (MDA) düzeyi ile total antioksidan kapasitesinin değerlendirilmesi ve lipid peroksidasyonun
hastalığın patojenezinde etkisinin araştırılması amaçlanmıştır. Klinik ve dışkı
muayenesinde koksidiyozis teşhisi konan 10 hayvan (hasta grubu) ile birlikte
aynı ahırda sağlıklı oldukları belirlenen benzer özellikteki 10 hayvandan
(kontrol grubu) oluşan toplam 20 baş besi danası araştırma materyalini oluşturmuştur.
Tüm hayvanların genel klinik muayene bulguları
(vücut sıcaklığı, kalp ve solunum frekansı ile rumen hareketleri) ve
hematolojik parametreleri (total lökosit sayısı, eritrosit sayısı, hematokrit
değer ve hemoglobin miktarı) ile serum MDA düzeyleri ve total antioksidan
kapasiteleri belirlenmiştir. Hasta
grubunda vücut
sıcaklığı, kalp frekansı, solunum frekansı, total lökosit sayısı ve serum MDA
düzeylerinin istatistiksel olarak önemli derecede arttığı, rumen hareketi, hematokrit
ve hemoglobin değeri ile serum
total antioksidan kapasitesinin düştüğü görülmüştür (P<0.05). Sonuç olarak,
MDA düzeyinin artmasına bağlı olarak savunma sisteminde bozulma olabileceğinden
koksidiyozisli hayvanlarda profilaktik tedbirlerin alınmasının gerekli olduğu
kanaatine varılmıştır. Bu parametrelerin tedaviyi etkin hale getirmenin yanı
sıra tanı koymak için diğer parametreler ile birlikte referans olarak
alınabileceğini gösterebilir.
References
- Aiello SE, Mays A (1998). The Merck Veterinary Manual. 8th edition, Merck Co, USA. Anderson DE, Rings DM (2009). Current Veterinary Food Animal Practice. 5th edition, Saunders Elsevier, Missouri. Argenzio RA, Rhoads JM (1997). Reactive oxygen metabolites in piglet cryptosporidiosis. Pediatr Res, 41, 521–526. Aumont G, Yvore P, Esnault, A (1986). Experimental coccidiosis in goats. 2. Effects of parasitism on nutritional balances and some blood parameters. Ann Rech Vet, 17, 191-196. Aydın UE, Aslan Ö (2012). Buzağı coccidiosis’inde bazı pıhtılaşma parametrelerinin belirlenmesi. Vet Hekim Der Derg, 83, 2, 1–8. Bangoura B, Daugschies A (2007). Parasitological and clinical parameters of experimental Eimeria zuernii infection in calves and infl uence on weight gain and haemogram. Parasitol Res, 100, 6, 1331–1340. Cam Y, Atasever A, Eraslan G, Kibar M, Atalay Ö, Beyaz L, Inci A, Liman BC (2008). Eimeria stiedae: Experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin. Exp Parasitol, 119, 164–172. Ching S, Ingram D, Hahnel R, Beilby J, Rossi E (2002). Serum levels of micronutrients, antioxidants and total antioxidant status predict risk of breast cancer in a case control study. J Nutr, 132, 303–306. Daugschies A, Najdrowski M (2005). Eimeriosis in cattle: Current understanding. J Vet Med B Infect Dis Vet Public Health, 52, 417-427. Dede S, Deger Y, Alkan M, Cemek M (2002). Oxidation products of nitric oxide and the concentration of antioxidant vitamins in parasitized goats. Acta Vet Brno, 71, 1–5. Dede S, Deger Y, Deger S, Alkan M (2000). Determination of the status of lipid peroxidation and antioxidants in sheep infected with certain endoparasites (Fasciola spp., Trichostronglidae spp., Eimeria spp.). Acta Parasit Turc, 24, 190–193. Dharmendra Kumar BV, Hafeez MD (1999). Efficacy of monensin and amprolium against subclinical coccidiosis in lambs. Indian Vet J, 1999; 76, 965–967. Dincer S (2001). Türkiye parazitoloji derneği. Meta Press, İzmir. Eraslan G, Cam Y, Eren M, Liman BC (2004). Changes in malondialdehyde level and catalase activity and effect of toltrazuril on these parameters in chicks infected with Eimeria tenella. B Vet I Pulawy, 48, 251–255. Erel O (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem, 37: 112–119. Erel O, Vural H, Aksoy N, Aslan G, Ulukanligil M (2001). Oxidative stress of platelets and thrombocytopenia in patients with vivax malaria. Clin Biochem, 34, 341–344. Georgi JR, Theodories VJ (1980). Parasitology for Veterinarians. 4th edition, W.B. Saunders Company, London. Georgieva NV, Koinarski V, Gadjeva V (2006). Antioxidant status during the course of Eimeria tenella infection in broiler chickens. Vet J, 172, 488–492.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | August 20, 2014 |
| Submission Date | February 5, 2014 |
| Acceptance Date | March 26, 2014 |
| Published in Issue | Year 2014 Volume: 25 Issue: 2 |
Cite
Accepted papers are licensed under Creative Commons Attribution-NonCommercial 4.0 International License