Histological and Histometric Structure of Goose (Anser anser) Cerebellum

Year 2018, Volume: 29 Issue: 2, 63 - 66, 20.09.2018

Serap Koral Taşçı

Abstract

The aim of this study is to examine the goose (Anser anser) cerebellum histometric and histologically. Six female
geese (aged between 10-12 months) were used in this study. After fixation in
10% formalin and following routine histologic processes, the cerebellar tissues
of geese were embedded in paraffin blocks and the sections were taken from
these blocks. The Kluver-Barrera nerve tissue stain technique was applied to
the sections. The ocular micrometer was used for histometric measurements of
folia length, diameter of purkinje cells and thicknesses of stratum granulosum
and stratum moleculare. SPSS 16.0 was used for calculating mean values. It was
observed histological that folia and sulci cerebelli were in the outermost
layer of the cerebellum. As a result of Kluver-Barrera staining, the nerve
cells in all layers appeared purple and myelin fibers appeared blue. It was
found that the average length of the folia was 3397.88 ± 931.32 µm, the average
thickness of molecular layer was 348.53 ± 72.92 µm, the average thickness of
granular layer was 184.83 ± 48.77 µm and the average diameter of Purkinje cells
was 24.23 ± 3.96 µm.

Keywords

Cerebellum, Goose, Kluver-Barrera, Purkinje cell

Kaz (Anser anser) Beyinciğinin Histolojik ve Histometrik Yapısı

Abstract

Bu çalışmada, kaz (Anser anser)
serebellum dokusunun histolojik ve histometrik yapısının incelenmesi amaçlandı.
Çalışmada 6 adet dişi kaz (10-12 aylık) kullanıldı. Kazlara ait serebellum
dokuları % 10’luk formalinde tespit edildikten sonra rutin histolojik
işlemlerden geçirilip parafinde bloklandı ve bu bloklardan kesitler alındı. Kesitlere
Kluver-Barrera sinir doku boyama tekniği uygulandı.  Folia uzunluğu, Purkinje hücre çapı, stratum
molekülare ve stratum granülosum katmanlarının kalınlıklarını ölçmek için
oküler mikrometre kullanıldı. Ortalama değerleri hesaplamak için SPSS 16.0 programı
kullanıldı. Yapılan histolojik incelemelerde serebellumun dış kısmında folia ve
sulkus serebelli yapıları gözlendi. Kluver-Barrera boyaması sonucunda tüm
katmanlardaki sinir hücreleri mor renkte, miyelinli lifler ise mavi renkte
görüldü. Foliaların ortalama uzunluğu 3397.88 ± 931.32 µm, stratum molekülare
katmanının ortalama kalınlığı 348.53 ± 72.92 µm, stratum granülosum katmanının
ortalama kalınlığı 184.83 ± 48.77 µm ve Purkinje hücrelerinin ortalama çapı ise
24.23 ± 3.96 µm olarak belirlendi.

Keywords

Kaz, Kluver-Barrera, Purkinje hücresi, Serebellum

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