Protective Mechanism of Urtica Dioica on Carbon Tetrachloride-Induced Hepatic Encephalopathy in Rats

Year 2018, Volume: 29 Issue: 2, 77 - 81, 20.09.2018

Gülşah Yıldız Deniz

Abstract

Around the world, species from the genus Urtica are commonly used because of their peripheral and central
medicinal effects; this is prepared as teas. In recent years, it has become
increasingly important to study the beneficial properties of derivatives of Urtica dioica (UD).  The aim of the
present study was to evaluate the effects of UD against carbon tetrachloride (CCl₄) induced hepatic encephalopathy (HE). Forty-nine adult (2-months-old) male Sprague dawley rats were used in this
study. The
models were established by CCl₄ 
(1 mL/kg body weight; twice a week) given intraperitoneally for 8
weeks. The animals were
euthanized by decapitation and rat brains were removed to assess histopathologic
changes. Biochemical parameters were assessed in serum samples from the CCl₄-treated
rats. UD extracts provided significant protection against CCl₄-induced
brain damage by increasing the preventing alterations in biochemical serum
parameters, such as the levels of aspartate aminotransferase (AST), alanine
aminotransferase (ALT), glutamyl transferase (GGT) and ammonia relative to
the control group. Histopathological and immunohistopathological changes of the
brain tissue was observed using Hematoxylin-eosin (H&E)
staining and c-Fos expression method. In the present
study, the protective effect of UD on CCl₄ toxicity was demonstrated through studies of biochemistry and immunohistopathology. Administration of UD may have
potential protective effects against CCl₄-induced brain toxicity.

Keywords

CCl4, Hepatic encephalopathy (HE), Rat, Urtica dioica

Urtica dioica'nın Sıçanlarda Karbon Tetraklorür ile İndüklenen Hepatik Ensefalopati Üzerine Koruyucu Mekanizması

Abstract

Dünya çapında, Urtica cinsinden türler periferik ve merkezi tıbbi
etkileri nedeniyle yaygın olarak kullanılmaktadır; bunlar çay olarak
hazırlanır. Son yıllarda, Urtica dioica
(UD) türevlerinin yararlı özelliklerini incelemek gittikçe önem kazanmıştır. Bu
çalışmanın amacı, UD'nin karbon tetraklorür (CCl4) ile indüklenen hepatik
ensefalopatiye (HE) karşı etkilerini değerlendirmektir. Bu çalışmada kırk dokuz
yetişkin (2 aylık) erkek Sprague dawley sıçanı kullanıldı. Modeller 8 hafta
boyunca intraperitoneal olarak verilen CCI₄ (1 mL / kg vücut
ağırlığı; haftada iki kez) ile oluşturuldu. Hayvanlar başları kesilerek ötanazi
edildi ve sıçan beyinleri histopatolojik değişiklikleri değerlendirmek için
çıkarıldı. Biyokimyasal parametreler CCI₄ ile tedavi edilen sıçanlardan
alınan serum örneklerinde değerlendirildi. UD ekstraktları, kontrol grubuna
göre aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), glutamil
transferaz (GGT) ve amonyak seviyeleri gibi biyokimyasal serum
parametrelerindeki önleyici değişiklikleri arttırarak CCI₄'ün neden
olduğu beyin hasarına karşı önemli bir koruma sağlamıştır. Hematoksilin-eozin
(H & E) boyama ve c-fos ekspresyon metodu kullanılarak beyin dokusunun
histopatolojik ve immünohistolojik değişiklikleri gözlendi. Bu çalışmada, UD'nin
CCI₄ toksisitesi üzerindeki koruyucu etkisi, biyokimya ve
immünohistoloji çalışmaları ile gösterilmiştir. UD'nin uygulanması CCI₄'ün
neden olduğu beyin toksisitesine karşı potansiyel koruyucu etkilere sahip
olabilir.

Keywords

Hepatik ensefalopati (HE), CCI4, Sıçan, Urtica dioica

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