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Biyokimyasal, Moleküler ve Histopatolojik Veriler Kullanılarak Likopenin Dietilnitrozamine Bağlı Kronik Hepatotoksisite Üzerine Koruyucu ve / veya Tedavi Edici Etkilerinin Araştırılması

Year 2020, Volume: 31 Issue: 1, 27 - 33, 12.03.2020
https://doi.org/10.36483/vanvetj.657440

Abstract

Çalışmanın amacı, likopenin dietilnitrozamin (DEN) kaynaklı kronik hepatotoksisite üzerindeki rolünü biyokimyasal, moleküler ve histopatolojik yaklaşımları kullanarak araştırmaktır. 35 adet erkek Wistar albino rat, her grupta 7 rat olacak şekilde 5 gruba ayrılmıştır. Gruplar, kontrol, likopen, DEN, likopen+DEN ve DEN+likopen şeklinde oluşturulmuştur. Likopen, gün aşırı olarak 10 mg/kg/vücut ağırlığı dozunda gavaj yoluyla 10 gün uygulanmıştır. DEN, ratlara 200 mg/kg vücut ağırlığı dozunda intraperitoneal olarak tek doz 90 gün uygulanmıştır. Likopen uygulaması, likopen+DEN grubunda DEN uygulamasından 10 gün önce, DEN+likopen grubunda ise DEN uygulaması ile birlikte başlamıştır. DEN uygulamasından 90 gün süre sonra çalışma sonlandırılmıştır. DEN, dokularda malondialdehid düzeylerinde artışa, redükte glutatyon düzeyi ve antioksidan enzim aktivitelerinde düşüşe sebep olarak oksidatif strese neden olmuştur (p<0,001). Likopen uygulaması hem kan hem de karaciğer dokusunda DEN grubuna kıyasla biyokimyasal endekslerde iyileşme sağlamıştır. RT-PCR analizlerinde DEN grubundaki katalaz enziminin ekspresyon düzeylerini arttırdığı belirlenmiştir. Histopatolojik olarak, DEN ve likopen+DEN gruplarının karaciğer dokularında karyomegali, nekroz ve hidropik dejenerasyon gibi birçok histopatolojik değişiklikler gözlenmiştir. Hem biyokimyasal hem de histopatolojik sonuçlarda DEN+likopen grubundaki iyileşmesinin likopen+DEN grubundan daha iyi olduğu gözlenmiştir. Bu sonuçlar, likopenin koruyucu etkisinden ziyade tedavi edici etkisinin DEN’e bağlı hepatotoksisitede likopenin antioksidan etkisine bağlı kaynaklandığını göstermektedir.

Supporting Institution

Fırat Üniversitesi Bilimsel Araştırma Projeleri (FÜBAP) Koordinasyon Birimi

Project Number

VF. 14. 19

References

  • Abdallah IZ, Khattab HA (2004). Protective role of lycopene against diethylnitrosamine induced experimental hepatocarcinogenesis. Egypt J Hosp Med, 16 (1), 1-13.
  • Aebi H (1984). Catalase in vitro. Meth Enzymol, 105, 121-126.
  • Agarwal S, Rao AV (2000). Tomato lycopene and its role in human health and chronic diseases. Can Med Assoc, 163 (6), 739-744.
  • Al-Rejaie SS, Aleisa MA, Al-Yahya AA, et al. (2009). Progression of diethylnitrosamine-induced hepatic carcinogenesis in carnitine-depleted rats. World J Gastroenterol, 15 (11), 1373-1380.
  • Amin A, Hamza AA, Bajbouj K, Ashraf SS, Daoud S. Saffron (2011). A potential candidate for a novel anticancer drug against hepatocellular carcinoma. Hepatology, 54 (3) 857-867.
  • Astorg P, Gradelet S, Bergès R, Suschetet M (1997). Dietary lycopene decreases the initiation of liver preneoplastic foci by diethylnitrosamine in the rat. Nutr Cancer, 29 (1), 60-68.
  • Beutler E (1984). Red Cell Metabolism. In: A Manual of Biochemical Methods. Grune and Starton (Editors), 2nd Edition, 160, New York.
  • Bingül I, Başaran-Küçükgergin C, Aydın AF et al. (2016). Blueberry treatment attenuated cirrhotic and preneoplastic lesions and oxidative stress in the liver of diethylnitrosamine-treated rats. Int J Immunopathol Pharm, 29(3), 426-437.
  • Blanche CIp, Hu KQ, Liu C, et al. (2013). Lycopene Metabolite, Apo-10′-Lycopenoic acid, inhibits diethylnitrosamine-initiated, high fat diet-promoted hepatic inflammation and tumorigenesis in mice. Cancer Prev Res, 6 (12), 1304-1316.
  • Britton G (1995). Structure and properties of carotenoids in relation to function. FASEB J, 9 (15), 1551-1558.
  • Dahl AR (1986). Aktivation of nitrosamines to mutagens by rat and rabbit nasal, lung and liver S-9 homogenates. Adv Exp Med Biol, 97, 367-372.
  • El-Agamey A, Lowe GM, McGarvey DJ, et al. (2004). Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys, 430 (1), 37-48.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol, 7, 88-95.
  • El-Shahat M, El-Abd S, Alkafafy M, El-Khatib G (2012). Potential chemoprevention of diethylnitrosamine-induced hepatocarcinogenesis in rats: Myrrh (Commiphora molmol) vs. turmeric (Curcuma longa). Acta Histochem, 114 (5), 421-428.
  • Farombi EO, Fakoya A (2005). Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers of Hibiscus sabdariffa L. Mol Nutr Food Res, 49 (12), 1120-1128.
  • Frankel SS, Reitman S, Sonnenwirth AC (1970). Grandwohl’s clinical laboratory methods and diagnosis. In: Reading CA, Glynn LE (Editors).7th Edition, 403-404, The C. V. Mosby Company, St Louis, USA.
  • Fustinoni-Reis AM, Arruda SF, Dourado LP, Cunha da MS, Siqueira E (2016). Tucum-Do-Cerrado (Bactris setosa Mart.) consumption modulates iron homeostasis and prevents iron-induced oxidative stress in the rat liver. Nutrients, 8 (2), 38.
  • Gaweł S, Wardas M, Niedworok E, Wardas P (2004). Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad Lek, 57 (9-10), 453-455.
  • Gayathri R, Priya DK, Gunassekaran GR, Sakthisekaran D (2009). Ursolic acid attenuates oxidative stress-mediated hepatocellular carcinoma induction by diethylnitrosamine in male Wistar rats. Asian Pac J Cancer Prev, 10 (5), 933-938.
  • Gupta P, Bansal PM, Koul A (2013). Lycopene modulates initiation of N-nitrosodiethylamine induced hepatocarcinogenesis: Studies on chromosomal abnormalities, membrane fluidity and antioxidant defense system. Chem Biol Interact, 206 (2), 364-374.
  • Habig WH, Pabst MJ, Jakoby WB (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249 (22), 7130-7139.
  • Halliwell B, Gutteridge JM (2015). Free Radicals in Biology and Medicine. Oxford University Press, USA.
  • Jahan MS, Vani G, Shyamaladevi CS (2007). Effect of Solanum trilobatum on hepatic drug metabolising enzymes during diethylnitrosamine‐induced hepatocarcinogenesis promoted by Phenobarbital in rat. Hepatol Res, 37 (1), 35-49.
  • Janani P, Sivakumari K, Geetha A, Ravisankar B, Parthasarathy C (2010). Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats. J Cancer Res Clin Oncol, 136 (5), 759-770.
  • Kim DJ, Takasuka N, Kim JM, et al. (1997). Chemoprevention by lycopene of mouse lung neoplasia after combined initiation treatment with DEN, MNU and DMH. Cancer Lett, 120 (1), 15-22.
  • Kim S, Choi JE, Choi J, et al. (2009). Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol in Vitro, 23 (6),1076-1084.
  • Klaunig JE, Wang Z, Pu X, Zhou S (2011). Oxidative stress and oxidative damage in chemical carcinogenesis. Toxicol Appl Pharmacol, 254 (2), 86-99.
  • Krinsky NI (1992). Mechanism of action of biological antioxidants. Proc Soc Exp Biol Med, 200 (2), 248-254.
  • Krinsky NI (1998). Overview of lycopene, carotenoids, and disease prevention. Proc Soc Exp Biol Med, 218 (2), 95-97.
  • Kubista M, Andrade JM, Bangtsson M, Forootan A (2006). The real-time polymerase chain reaction. Mol Aspects Med, 27 (2-3), 95-125.
  • Kumar P, Kumar A (2009). Effect of lycopene and epigallocatechin-3-gallate against 3-nitropropionic acid induced cognitive dysfunction and glutathione depletion in rat: a novel nitric oxide mechanism. Food Chem Toxicol, 47 (10), 2522-2530.
  • Lijinsky W (1992). Chemistry and Biology of N-nitroso Compounds. Cambridge, UK: Cambridge Univ. Pres.
  • Lijinsky W, Kovatch RM (1989). Carcinogenesis by nitrosamines and azoxyalkanes by different routes of administration to rats. Biomed Environ Sci, 2 (2), 154-159.
  • Liu LL, Gong LK, Qı XM, et al. (2005). Altered expression of cytochrome p450 and possible correlation with preneoplastic changes in early stage of rat hepatocarcinogenesis. Acta Pharmacol Sin, 26 (6), 737-744.
  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the folin-phenol reagent. J Biol Chem, 193, 265-257.
  • Luna LG (1968). Manuel of histologic staining methods of Armed Forces Institute of Pathology. 1-36, McGraw-Hill Book Co, New York.
  • Matsuda M, Nakamoto Y, Suzuki S, Kurata T, Kaneko S (2005). Interferon-c-mediated hepatocarcinogenesis in mice treated with diethylnitrosamine. Lab Invest, 85 (5), 655-663.
  • Mayne ST (1996). Beta-carotene, carotenoids, and disease prevention in humans. FASEB J, 10 (7), 690-701.
  • Mitacek EJ, Brunnemann KD, Suttajit M, et al. (1999). Exposure to N-nitroso compounds in a population of high liver cancer regions in Thailand: volatile nitrosamine (VNA) levels in Thai food. Food Chem Toxicol, 37 (4), 297-305.
  • Nagahara T, Okano J, Fujise Y, Abe R, Murawaki Y (2010). Preventive effect of JTE-522, a selective cyclooxygenase-2 inhibitor, on DEN-induced hepatocarcinogenesis in rats. Biomed Pharmacother, 64 (5), 319-326.
  • Placer ZA, Cushman L, Johnson BC (1966). Estimation of products of lipid peroxidation in biological fluids. Anal Biochem, 16: 359-364.
  • Pradeep K, Mohan CVR, Gobianand K, Karthikeyan S (2007). Silymarin modulates the oxidant-antioxidant imbalance during diethylnitrosamine induced oxidative stress in rats. Euro J Pharmacol, 560 (2-3), 110-116.
  • Pradeep K, Raj Mohan CV, Gobianand K, Karthikeyan S (2010). Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats. Biol Res, 43 (1), 113-125.
  • Qu M, Nan X, Gao Z, et al. (2013). Protective effects of lycopene against methylmercury-induced neurotoxicity in cultured rat cerebellar granule neurons. Brain Res, 1540, 92-102.
  • Ramakrishnan G, Raghavendran HRB, Vinodhkumar R, Devaki T (2006). Suppression of N-nitrosodiethylamine induced hepatocarcinogenesis by silymarin in rats. Chem Biol, 161 (2), 104-114.
  • Rotimi OA, Rotimi SO, Duru CU, et al. (2017). Acute aflatoxin B1–Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats. Toxicol Rep, 4, 408-414.
  • Sahin K, Orhan C, Tuzcu M, et al. (2014). Orally administered lycopene attenuates diethylnitrosamine-induced hepatocarcinogenesis in rats by modulating Nrf-2/HO-1 and Akt/mTOR pathways. Nutr Cancer, 66 (4), 590-598.
  • Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S, et al. (2010). Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxid Med Cell Longev, 3 (4), 254-261.
  • Shaarawy SM, Tohamy AA, Elgendy SM, et al. (2009). Protective effects of garlic and silymarin on NDEA-induced rats hepatotoxicity. Int J Biol Sci, 5 (6), 549-557.
  • Sun Y, Oberly LW, Ying LA (1988). Simple method for clinical assay of superoxide dismutase. Clin Chem, 34 (3), 497-500.
  • Taha MM, Abdul AB, Abdullah R, et al. (2010). Potential chemoprevention of diethylnitrosamine-initiated and 2-acetylaminofluorene-promoted hepatocarcinogenesis by zerumbone from the rhizomes of the subtropical ginger (Zingiber zerumbet). Chem Biol Interact, 186 (3), 295-305.
  • Ukai N, Lu Y, Etoh H, et al. (1994). Photosensitized oxygenation of lycopene. Biosci Biotech Biochem, 58 (9), 1718-1719.
  • Vineis P, Wild CP (2014). Global cancer patterns: causes and prevention. Lancet, 383 (9916), 549-557.
  • Wang Y, Ausman LM, Greenberg AS, Russell RM, Wang XD (2010). Dietary lycopene and tomato extract supplementations inhibit nonalcoholic steatohepatitis-promoted hepatocarcinogenesis in rats. Int J Cancer, 126 (8), 1788-1796.
  • Yamada K, Yamamiya I, Utsumi H (2006). In vivo detection of free radicals induced by diethylnitrosamine in rat liver tissue. Free Radic Biol Med, 40 (11), 2040-2046.
  • Yılmaz S, Karahan İ, Kandemir FM (2008). Bazı nitrozaminlerin ratlarda doku pirüvat kinaz aktivitesi üzerine etkileri. F.Ü. Sağ Bil Derg, 22 (3), 163-168.
  • Yilmaz S, Atessahin A, Sahna E, Karahan I, Ozer S (2006). Protective effect of lycopene on adriamycin-induced cardiotoxicity and nephrotoxicity. Toxicology, 218 (2), 164-171.

Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data

Year 2020, Volume: 31 Issue: 1, 27 - 33, 12.03.2020
https://doi.org/10.36483/vanvetj.657440

Abstract

The aim of the study is to investigate the role of lycopene on diethylnitrosamine (DEN)-induced chronic hepatotoxicity using biochemical, molecular and histopathological approaches. Thirty five male Wistar albino rats were assigned into five groups of 7 rats each. Groups were formed as control, lycopene, DEN, lycopene+DEN and DEN+lycopene. Lycopene was applied to rats every other day at 10 mg/kg/bw, gavage for 10 days. DEN was applied intraperitoneally to rats at a single dose, 200 mg/kg/bw for 90 days. Lycopene administration was started 10 days before the DEN administration in lycopene+DEN group, together with the DEN administration in DEN+lycopene group. The study was terminated 90 days after DEN administration. DEN caused the oxidative stress by the increased malondialdehyde level and the decreased reduced glutathione level, antioxidant enzyme activities (p<0.001). Lycopene administration improved the biochemical indices of both blood and liver tissue compared to the DEN group. RT-PCR analysis revealed that the catalase enzyme in the DEN group increased expression levels. Histopathologically, many histopathologic changes such as karyomegaly, necrosis and hydropic degeneration were observed in the liver tissues of the DEN and lycopene+DEN groups. Both biochemical and histopathological results showed that healing of DEN+lycopene group was better than lycopene+DEN group. These results suggest that besides the protective effects, the therapeutic effect of lycopene is due to its antioxidant effects on DEN‐induced hepatotoxicity.

Project Number

VF. 14. 19

References

  • Abdallah IZ, Khattab HA (2004). Protective role of lycopene against diethylnitrosamine induced experimental hepatocarcinogenesis. Egypt J Hosp Med, 16 (1), 1-13.
  • Aebi H (1984). Catalase in vitro. Meth Enzymol, 105, 121-126.
  • Agarwal S, Rao AV (2000). Tomato lycopene and its role in human health and chronic diseases. Can Med Assoc, 163 (6), 739-744.
  • Al-Rejaie SS, Aleisa MA, Al-Yahya AA, et al. (2009). Progression of diethylnitrosamine-induced hepatic carcinogenesis in carnitine-depleted rats. World J Gastroenterol, 15 (11), 1373-1380.
  • Amin A, Hamza AA, Bajbouj K, Ashraf SS, Daoud S. Saffron (2011). A potential candidate for a novel anticancer drug against hepatocellular carcinoma. Hepatology, 54 (3) 857-867.
  • Astorg P, Gradelet S, Bergès R, Suschetet M (1997). Dietary lycopene decreases the initiation of liver preneoplastic foci by diethylnitrosamine in the rat. Nutr Cancer, 29 (1), 60-68.
  • Beutler E (1984). Red Cell Metabolism. In: A Manual of Biochemical Methods. Grune and Starton (Editors), 2nd Edition, 160, New York.
  • Bingül I, Başaran-Küçükgergin C, Aydın AF et al. (2016). Blueberry treatment attenuated cirrhotic and preneoplastic lesions and oxidative stress in the liver of diethylnitrosamine-treated rats. Int J Immunopathol Pharm, 29(3), 426-437.
  • Blanche CIp, Hu KQ, Liu C, et al. (2013). Lycopene Metabolite, Apo-10′-Lycopenoic acid, inhibits diethylnitrosamine-initiated, high fat diet-promoted hepatic inflammation and tumorigenesis in mice. Cancer Prev Res, 6 (12), 1304-1316.
  • Britton G (1995). Structure and properties of carotenoids in relation to function. FASEB J, 9 (15), 1551-1558.
  • Dahl AR (1986). Aktivation of nitrosamines to mutagens by rat and rabbit nasal, lung and liver S-9 homogenates. Adv Exp Med Biol, 97, 367-372.
  • El-Agamey A, Lowe GM, McGarvey DJ, et al. (2004). Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys, 430 (1), 37-48.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol, 7, 88-95.
  • El-Shahat M, El-Abd S, Alkafafy M, El-Khatib G (2012). Potential chemoprevention of diethylnitrosamine-induced hepatocarcinogenesis in rats: Myrrh (Commiphora molmol) vs. turmeric (Curcuma longa). Acta Histochem, 114 (5), 421-428.
  • Farombi EO, Fakoya A (2005). Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers of Hibiscus sabdariffa L. Mol Nutr Food Res, 49 (12), 1120-1128.
  • Frankel SS, Reitman S, Sonnenwirth AC (1970). Grandwohl’s clinical laboratory methods and diagnosis. In: Reading CA, Glynn LE (Editors).7th Edition, 403-404, The C. V. Mosby Company, St Louis, USA.
  • Fustinoni-Reis AM, Arruda SF, Dourado LP, Cunha da MS, Siqueira E (2016). Tucum-Do-Cerrado (Bactris setosa Mart.) consumption modulates iron homeostasis and prevents iron-induced oxidative stress in the rat liver. Nutrients, 8 (2), 38.
  • Gaweł S, Wardas M, Niedworok E, Wardas P (2004). Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad Lek, 57 (9-10), 453-455.
  • Gayathri R, Priya DK, Gunassekaran GR, Sakthisekaran D (2009). Ursolic acid attenuates oxidative stress-mediated hepatocellular carcinoma induction by diethylnitrosamine in male Wistar rats. Asian Pac J Cancer Prev, 10 (5), 933-938.
  • Gupta P, Bansal PM, Koul A (2013). Lycopene modulates initiation of N-nitrosodiethylamine induced hepatocarcinogenesis: Studies on chromosomal abnormalities, membrane fluidity and antioxidant defense system. Chem Biol Interact, 206 (2), 364-374.
  • Habig WH, Pabst MJ, Jakoby WB (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249 (22), 7130-7139.
  • Halliwell B, Gutteridge JM (2015). Free Radicals in Biology and Medicine. Oxford University Press, USA.
  • Jahan MS, Vani G, Shyamaladevi CS (2007). Effect of Solanum trilobatum on hepatic drug metabolising enzymes during diethylnitrosamine‐induced hepatocarcinogenesis promoted by Phenobarbital in rat. Hepatol Res, 37 (1), 35-49.
  • Janani P, Sivakumari K, Geetha A, Ravisankar B, Parthasarathy C (2010). Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats. J Cancer Res Clin Oncol, 136 (5), 759-770.
  • Kim DJ, Takasuka N, Kim JM, et al. (1997). Chemoprevention by lycopene of mouse lung neoplasia after combined initiation treatment with DEN, MNU and DMH. Cancer Lett, 120 (1), 15-22.
  • Kim S, Choi JE, Choi J, et al. (2009). Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol in Vitro, 23 (6),1076-1084.
  • Klaunig JE, Wang Z, Pu X, Zhou S (2011). Oxidative stress and oxidative damage in chemical carcinogenesis. Toxicol Appl Pharmacol, 254 (2), 86-99.
  • Krinsky NI (1992). Mechanism of action of biological antioxidants. Proc Soc Exp Biol Med, 200 (2), 248-254.
  • Krinsky NI (1998). Overview of lycopene, carotenoids, and disease prevention. Proc Soc Exp Biol Med, 218 (2), 95-97.
  • Kubista M, Andrade JM, Bangtsson M, Forootan A (2006). The real-time polymerase chain reaction. Mol Aspects Med, 27 (2-3), 95-125.
  • Kumar P, Kumar A (2009). Effect of lycopene and epigallocatechin-3-gallate against 3-nitropropionic acid induced cognitive dysfunction and glutathione depletion in rat: a novel nitric oxide mechanism. Food Chem Toxicol, 47 (10), 2522-2530.
  • Lijinsky W (1992). Chemistry and Biology of N-nitroso Compounds. Cambridge, UK: Cambridge Univ. Pres.
  • Lijinsky W, Kovatch RM (1989). Carcinogenesis by nitrosamines and azoxyalkanes by different routes of administration to rats. Biomed Environ Sci, 2 (2), 154-159.
  • Liu LL, Gong LK, Qı XM, et al. (2005). Altered expression of cytochrome p450 and possible correlation with preneoplastic changes in early stage of rat hepatocarcinogenesis. Acta Pharmacol Sin, 26 (6), 737-744.
  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the folin-phenol reagent. J Biol Chem, 193, 265-257.
  • Luna LG (1968). Manuel of histologic staining methods of Armed Forces Institute of Pathology. 1-36, McGraw-Hill Book Co, New York.
  • Matsuda M, Nakamoto Y, Suzuki S, Kurata T, Kaneko S (2005). Interferon-c-mediated hepatocarcinogenesis in mice treated with diethylnitrosamine. Lab Invest, 85 (5), 655-663.
  • Mayne ST (1996). Beta-carotene, carotenoids, and disease prevention in humans. FASEB J, 10 (7), 690-701.
  • Mitacek EJ, Brunnemann KD, Suttajit M, et al. (1999). Exposure to N-nitroso compounds in a population of high liver cancer regions in Thailand: volatile nitrosamine (VNA) levels in Thai food. Food Chem Toxicol, 37 (4), 297-305.
  • Nagahara T, Okano J, Fujise Y, Abe R, Murawaki Y (2010). Preventive effect of JTE-522, a selective cyclooxygenase-2 inhibitor, on DEN-induced hepatocarcinogenesis in rats. Biomed Pharmacother, 64 (5), 319-326.
  • Placer ZA, Cushman L, Johnson BC (1966). Estimation of products of lipid peroxidation in biological fluids. Anal Biochem, 16: 359-364.
  • Pradeep K, Mohan CVR, Gobianand K, Karthikeyan S (2007). Silymarin modulates the oxidant-antioxidant imbalance during diethylnitrosamine induced oxidative stress in rats. Euro J Pharmacol, 560 (2-3), 110-116.
  • Pradeep K, Raj Mohan CV, Gobianand K, Karthikeyan S (2010). Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats. Biol Res, 43 (1), 113-125.
  • Qu M, Nan X, Gao Z, et al. (2013). Protective effects of lycopene against methylmercury-induced neurotoxicity in cultured rat cerebellar granule neurons. Brain Res, 1540, 92-102.
  • Ramakrishnan G, Raghavendran HRB, Vinodhkumar R, Devaki T (2006). Suppression of N-nitrosodiethylamine induced hepatocarcinogenesis by silymarin in rats. Chem Biol, 161 (2), 104-114.
  • Rotimi OA, Rotimi SO, Duru CU, et al. (2017). Acute aflatoxin B1–Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats. Toxicol Rep, 4, 408-414.
  • Sahin K, Orhan C, Tuzcu M, et al. (2014). Orally administered lycopene attenuates diethylnitrosamine-induced hepatocarcinogenesis in rats by modulating Nrf-2/HO-1 and Akt/mTOR pathways. Nutr Cancer, 66 (4), 590-598.
  • Sayed-Ahmed MM, Aleisa AM, Al-Rejaie S, et al. (2010). Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxid Med Cell Longev, 3 (4), 254-261.
  • Shaarawy SM, Tohamy AA, Elgendy SM, et al. (2009). Protective effects of garlic and silymarin on NDEA-induced rats hepatotoxicity. Int J Biol Sci, 5 (6), 549-557.
  • Sun Y, Oberly LW, Ying LA (1988). Simple method for clinical assay of superoxide dismutase. Clin Chem, 34 (3), 497-500.
  • Taha MM, Abdul AB, Abdullah R, et al. (2010). Potential chemoprevention of diethylnitrosamine-initiated and 2-acetylaminofluorene-promoted hepatocarcinogenesis by zerumbone from the rhizomes of the subtropical ginger (Zingiber zerumbet). Chem Biol Interact, 186 (3), 295-305.
  • Ukai N, Lu Y, Etoh H, et al. (1994). Photosensitized oxygenation of lycopene. Biosci Biotech Biochem, 58 (9), 1718-1719.
  • Vineis P, Wild CP (2014). Global cancer patterns: causes and prevention. Lancet, 383 (9916), 549-557.
  • Wang Y, Ausman LM, Greenberg AS, Russell RM, Wang XD (2010). Dietary lycopene and tomato extract supplementations inhibit nonalcoholic steatohepatitis-promoted hepatocarcinogenesis in rats. Int J Cancer, 126 (8), 1788-1796.
  • Yamada K, Yamamiya I, Utsumi H (2006). In vivo detection of free radicals induced by diethylnitrosamine in rat liver tissue. Free Radic Biol Med, 40 (11), 2040-2046.
  • Yılmaz S, Karahan İ, Kandemir FM (2008). Bazı nitrozaminlerin ratlarda doku pirüvat kinaz aktivitesi üzerine etkileri. F.Ü. Sağ Bil Derg, 22 (3), 163-168.
  • Yilmaz S, Atessahin A, Sahna E, Karahan I, Ozer S (2006). Protective effect of lycopene on adriamycin-induced cardiotoxicity and nephrotoxicity. Toxicology, 218 (2), 164-171.
There are 57 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Araştırma Makaleleri
Authors

Emre Kaya

Seval Yılmaz 0000-0001-5472-3560

Ali Osman Çeribaşı 0000-0002-6096-4042

Selda Telo 0000-0003-3655-0269

Project Number VF. 14. 19
Publication Date March 12, 2020
Submission Date December 12, 2019
Acceptance Date January 27, 2020
Published in Issue Year 2020 Volume: 31 Issue: 1

Cite

APA Kaya, E., Yılmaz, S., Çeribaşı, A. O., Telo, S. (2020). Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data. Van Veterinary Journal, 31(1), 27-33. https://doi.org/10.36483/vanvetj.657440
AMA Kaya E, Yılmaz S, Çeribaşı AO, Telo S. Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data. Van Vet J. March 2020;31(1):27-33. doi:10.36483/vanvetj.657440
Chicago Kaya, Emre, Seval Yılmaz, Ali Osman Çeribaşı, and Selda Telo. “Investigation of the Protective and / Or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data”. Van Veterinary Journal 31, no. 1 (March 2020): 27-33. https://doi.org/10.36483/vanvetj.657440.
EndNote Kaya E, Yılmaz S, Çeribaşı AO, Telo S (March 1, 2020) Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data. Van Veterinary Journal 31 1 27–33.
IEEE E. Kaya, S. Yılmaz, A. O. Çeribaşı, and S. Telo, “Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data”, Van Vet J, vol. 31, no. 1, pp. 27–33, 2020, doi: 10.36483/vanvetj.657440.
ISNAD Kaya, Emre et al. “Investigation of the Protective and / Or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data”. Van Veterinary Journal 31/1 (March 2020), 27-33. https://doi.org/10.36483/vanvetj.657440.
JAMA Kaya E, Yılmaz S, Çeribaşı AO, Telo S. Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data. Van Vet J. 2020;31:27–33.
MLA Kaya, Emre et al. “Investigation of the Protective and / Or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data”. Van Veterinary Journal, vol. 31, no. 1, 2020, pp. 27-33, doi:10.36483/vanvetj.657440.
Vancouver Kaya E, Yılmaz S, Çeribaşı AO, Telo S. Investigation of the Protective and / or Therapeutic Effects of Lycopene on Diethylnitrosamine-Induced Chronic Hepatotoxicity Using Biochemical, Molecular and Histopathological Data. Van Vet J. 2020;31(1):27-33.

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