Year 2020, Volume 31 , Issue 2, Pages 105 - 110 2020-07-03

Ratlarda Dietilnitrozamin Uygulanarak Oluşturulan Oksidatif ve Nitrozatif Strese Karşı Silimarin’in Karaciğer Koruyucu Etkisi
Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats

Emin KARAKURT [1] , Serpil DAĞ [2]


Karaciğer kanseri dünyada kanserle ilişkili ölümlerde üçüncü sırada yer almaktadır. Hepatoselüler karsinom (HSK) primer karaciğer kanserlerinin %70-85’ini oluşturmaktadır. Bu çalışmada; deneysel karaciğer kanseri modelinde Dietilnitrozamin (DEN)'in rolünü araştırmayı ve silimarinin hepatoprotektif etkisini oksidatif-nitrozatif stres markerlerı kullanarak immunohistokimyasal yöntemlerle ayrıntılı olarak incelemeyi amaçladık. Bu amaçla 50 adet Wistar Albino cinsi erkek rat 10’arlı 5 gruba ayrıldı. DEN grubundaki hayvanlara 50 mg/kg dozunda haftada 1 kez deneme süresince DEN uygulaması yapıldı. DEN+silimarin grubundaki ratlara DEN uygulamasından 1 hafta önce başlayacak şekilde 100 mg/kg dozunda haftada 3 kez deneme süresince intraperitoneal yolla silimarin (propilen glikolde çözdürülmüş) uygulandı. Kontrol grubundaki ratlara deneme süresince pelet yem ve içme suyu verildi. Sham grubundaki hayvanlara % 0.9 NaCl içerisinde çözdürülmüş propilen glikol intraperitoneal yolla haftada 3 kez deneme süresince verildi. Silimarin grubundaki ratlara 100 mg/kg dozunda deneme süresince intraperitoneal yolla haftada 3 kez silimarin uygulandı. 21 haftanın sonunda DEN ve DEN+silimarin gruplarında makroskobik olarak farklı büyüklük ve boyutlarda nodüler yapılar tespit edildi. Mikroskobik incelemede nodüllerin HSK oldukları belirlendi. Sonuç olarak DEN’in karaciğer kanserini indüklediği; silimarinin ise iNOS ve Nitrotirozin ekspresyonları açısından istatistiksel olarak anlamlı bir koruyucu etki göstermediği belirlenmiştir.
Liver cancer is the third most common cancer-related death in the World. Hepatocellular carcinoma (HCC) constitutes %70-85 of liver malignant primary tumors. In this study; we aimed to investigate the role of diethylnitrosamine (DEN) in the experimental liver cancer model and to examine the hepatoprotective effect of silymarin in detail using oxidative-nitrosative stress markers with immunohistochemical methods. For this purpose, 50 Wistar Albino male rats were divided into 5 groups with 10 rats. Animals in DEN group received DEN at a dose of 50 mg/kg once a week for 20 weeks. The rats in the DEN +Silymarin group were Silymarin intraperitoneally for 21 weeks 3 times a week at a dose of 100 mg/kg, starting 1 week prior to the administration of DEN. Silymarin was administered to rats in the group of Silymarin 3 times a week intraperitoneally for 20 weeks at a dose of 100 mg/kg. The rats in the control group were fed pellet feed and drinking water for 20 weeks. Animals in Sham group were treated with propylene glycol dissolved in 0,9 % NaCl intraperitoneally 3 times a week for 20 weeks. At the end of 21 weeks, macroscopically different size and number of nodular structures were detected in the DEN and DEN+Silymarin group. The microscopic examination revealed that the nodules were HCC. As a result, DEN induced liver cancer; however, silymarin did not show a statistically significant protective effect in terms of iNOS and Nitrotyrosine expressions.
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Primary Language en
Subjects Veterinary
Journal Section Araştırma Makaleleri
Authors

Orcid: 0000-0003-2019-3690
Author: Emin KARAKURT (Primary Author)
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-7667-689X
Author: Serpil DAĞ
Institution: KAFKAS UNIVERSITY
Country: Turkey


Supporting Institution KAÜ Bilimsel Araştırma Projeleri Koordinatörlüğü
Project Number 2016-TS-08
Thanks Bu çalışma KAÜ Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenmiştir. Proje No: 2016-TS-08
Dates

Application Date : January 27, 2020
Acceptance Date : April 30, 2020
Publication Date : July 3, 2020

Bibtex @research article { vanvetj680365, journal = {Van Veterinary Journal}, issn = {2149-3359}, eissn = {2149-8644}, address = {YYÜ Veteriner Fakültesi Dergi Editörlüğü Kampüs Van Turkey}, publisher = {Yuzuncu Yil University}, year = {2020}, volume = {31}, pages = {105 - 110}, doi = {10.36483/vanvetj.680365}, title = {Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats}, key = {cite}, author = {Karakurt, Emin and Dağ, Serpil} }
APA Karakurt, E , Dağ, S . (2020). Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats . Van Veterinary Journal , 31 (2) , 105-110 . DOI: 10.36483/vanvetj.680365
MLA Karakurt, E , Dağ, S . "Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats" . Van Veterinary Journal 31 (2020 ): 105-110 <https://dergipark.org.tr/en/pub/vanvetj/issue/55797/680365>
Chicago Karakurt, E , Dağ, S . "Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats". Van Veterinary Journal 31 (2020 ): 105-110
RIS TY - JOUR T1 - Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats AU - Emin Karakurt , Serpil Dağ Y1 - 2020 PY - 2020 N1 - doi: 10.36483/vanvetj.680365 DO - 10.36483/vanvetj.680365 T2 - Van Veterinary Journal JF - Journal JO - JOR SP - 105 EP - 110 VL - 31 IS - 2 SN - 2149-3359-2149-8644 M3 - doi: 10.36483/vanvetj.680365 UR - https://doi.org/10.36483/vanvetj.680365 Y2 - 2020 ER -
EndNote %0 Van Veterinary Journal Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats %A Emin Karakurt , Serpil Dağ %T Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats %D 2020 %J Van Veterinary Journal %P 2149-3359-2149-8644 %V 31 %N 2 %R doi: 10.36483/vanvetj.680365 %U 10.36483/vanvetj.680365
ISNAD Karakurt, Emin , Dağ, Serpil . "Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats". Van Veterinary Journal 31 / 2 (July 2020): 105-110 . https://doi.org/10.36483/vanvetj.680365
AMA Karakurt E , Dağ S . Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats. Van Vet J. 2020; 31(2): 105-110.
Vancouver Karakurt E , Dağ S . Hepatoprotective Effect Of Silymarin Against Diethylnitrosamine Induced Oxidative-Nitrosative Stress in Rats. Van Veterinary Journal. 2020; 31(2): 105-110.