Year 2020, Volume 31 , Issue 2, Pages 111 - 116 2020-07-03

Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium
Kadmiyuma Maruz Kalan Ratlarda Oral Melatonin Uygulamasının Oksidatif Stres ve Bağırsak Mikroflorası Üzerine Etkisi

Hakan TAVŞANLI [1] , İ̇hsan KISADERE [2]


The aim of the present study was to evaluate the effects of oral melatonin treatment on oxidative stress and intestinal microflora in rats exposed to chronic cadmium. Healthy 32 adult male albino Wistar rats were randomly divided into four groups as control (C; n=8), cadmium (Cd; n=8), melatonin (Mlt, n=8), cadmium + melatonin (Cd + Mlt; n=8). Mlt (100 mg/kg) was orally administered 5 times (Mlt and Cd + Mlt), and CdCl2 (2 mg/day) 3 times a week for 4 weeks to rats (Cd and Cd + Mlt). After the treatments, serum total antioxidant status (TAS), total oxidant (TOS) levels as well as plasma ALT, AST, GGT, T.pro, Alb and BUN values were measured. Intestinal contents were aseptically collected, and Enterobacteriaceae, Lactococcus spp. and Lactobacillus spp. counts were performed. As a result, serum TOS levels were defined higher in Cd group than other groups (P <0.05). Lactococcus spp. counts decreased as 0.63 log in Cd group compared to C in small intestine, however it increased as 1.15 log in Cd + Mlt group. In conclusion, Cd + Mlt chelate was found benefical for intestinal microflora due to suppressed the Enterobacteriaceae growth, however ameliorated the Cd induced oxidative stress and Lactobacillus spp./Lactococcus spp. rates in the different part of the intestine.

Bu çalışmanın amacı, kronik kadmiyuma maruz kalan ratlarda oral melatonin uygulamasının, oksidatif stres ve bağırsak mikroflorasında bulunan bazı mikroorganizmalar üzerine etkilerini araştırmaktır. Sağlıklı 32 yetişkin erkek albino Wistar rat, kontrol (C; n=8), kadmiyum (Cd; n=8), melatonin (Mlt, n=8), kadmiyum + melatonin (Cd + Mlt; n=8) olarak rastgele dört gruba ayrılmıştır. Ratlara oral yolla Mlt (100 mg/kg), (Cd ve Cd + Mlt) haftada 5 kez (Mlt ve Cd + Mlt) ve CdCl2 (2 mg/gün) 3 kez olmak üzere 4 hafta boyunca uygulanmıştır. Uygulamadan sonra serum total antioksidan seviyeleri (TAS), total oksidan (TOS) düzeyleri ile plazma ALT, AST, GGT, T.pro, Alb ve BUN değerleri ölçülmüştür. Ayrıca ince ve kalın bağırsak içerikleri ayrı ayrı aseptik olarak alınarak bağırsak florasında Enterobacteriaceae, Lactococcus spp. ve Lactobacillus spp. sayımları yapılmıştır. Bulgu olarak, serum TOS düzeyleri Cd grubunda diğer gruplara göre daha yüksek olarak tanımlanmıştır (P <0,05). Cd grubunda ince bağırsak florasında Lactococcus spp. sayısı C grubuna göre 0,63 log azalırken, Cd + Mlt grubunda 1,15 log artmıştır. Sonuç olarak, Cd + Mlt şelatının Enterobacteriaceae üremesini baskılanması, Lactobacillus spp./Lactococcus spp. oranını düzenlemesi ve Cd’a bağlı gelişen oksidatif stresi azaltması nedeni ile intestinal mikroflora için faydalı olabilir.

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Primary Language en
Subjects Veterinary
Journal Section Araştırma Makaleleri
Authors

Orcid: 0000-0002-5124-3702
Author: Hakan TAVŞANLI (Primary Author)
Institution: BALIKESİR ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ
Country: Turkey


Orcid: 0000-0003-0732-0464
Author: İ̇hsan KISADERE
Institution: FACULTY OF VETERINARY
Country: Turkey


Dates

Application Date : February 5, 2020
Acceptance Date : May 11, 2020
Publication Date : July 3, 2020

Bibtex @research article { vanvetj685081, journal = {Van Veterinary Journal}, issn = {2149-3359}, eissn = {2149-8644}, address = {YYÜ Veteriner Fakültesi Dergi Editörlüğü Kampüs Van Turkey}, publisher = {Yuzuncu Yil University}, year = {2020}, volume = {31}, pages = {111 - 116}, doi = {10.36483/vanvetj.685081}, title = {Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium}, key = {cite}, author = {Tavşanlı, Hakan and Kısadere, İ̇hsan} }
APA Tavşanlı, H , Kısadere, İ . (2020). Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium . Van Veterinary Journal , 31 (2) , 111-116 . DOI: 10.36483/vanvetj.685081
MLA Tavşanlı, H , Kısadere, İ . "Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium" . Van Veterinary Journal 31 (2020 ): 111-116 <https://dergipark.org.tr/en/pub/vanvetj/issue/55797/685081>
Chicago Tavşanlı, H , Kısadere, İ . "Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium". Van Veterinary Journal 31 (2020 ): 111-116
RIS TY - JOUR T1 - Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium AU - Hakan Tavşanlı , İ̇hsan Kısadere Y1 - 2020 PY - 2020 N1 - doi: 10.36483/vanvetj.685081 DO - 10.36483/vanvetj.685081 T2 - Van Veterinary Journal JF - Journal JO - JOR SP - 111 EP - 116 VL - 31 IS - 2 SN - 2149-3359-2149-8644 M3 - doi: 10.36483/vanvetj.685081 UR - https://doi.org/10.36483/vanvetj.685081 Y2 - 2020 ER -
EndNote %0 Van Veterinary Journal Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium %A Hakan Tavşanlı , İ̇hsan Kısadere %T Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium %D 2020 %J Van Veterinary Journal %P 2149-3359-2149-8644 %V 31 %N 2 %R doi: 10.36483/vanvetj.685081 %U 10.36483/vanvetj.685081
ISNAD Tavşanlı, Hakan , Kısadere, İ̇hsan . "Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium". Van Veterinary Journal 31 / 2 (July 2020): 111-116 . https://doi.org/10.36483/vanvetj.685081
AMA Tavşanlı H , Kısadere İ . Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium. Van Vet J. 2020; 31(2): 111-116.
Vancouver Tavşanlı H , Kısadere İ . Influence of oral melatonin administration on oxidative stress and intestinal microflora in rats exposed to cadmium. Van Veterinary Journal. 2020; 31(2): 111-116.