Keklik ve Bıldırcın Konjunktiva Epitelindeki Glikokonjugatların Lektin Histokimyası

Year 2021, Volume: 32 Issue: 1, 1 - 6, 25.03.2021

Mustafa Öztop , Kenan Çınar , Emel Demirbağ

https://doi.org/10.36483/vanvetj.558834

Cited By: 1

Abstract

Bu çalışmada keklik (Alectoris chukar) ve bıldırcın (Coturnix coturnix) konjonktiva epitelindeki glikokonjugatların dağılımının belirlenmesi amaçlandı. Her iki türden alınan gözkapağı örnekleri rutin doku takibi işlemlerinden geçildi. Glikokonjugat ekspresyonunu göstermek için alınan kesitlere lektin histokimyası uygulandı. Bu amaçla altı farklı HRP-bağlı lektin (Con A, UEA-I, PNA, HPA, MAA ve BSA I-B4) kullanıldı. Hem keklikte hem de bıldırcında konjunktiva epiteli, goblet hücreleri ve goblet olmayan hücrelerden oluşmaktadır. Hem PNA hem de HPA değişen derecelerde bıldırcın ve keklik konjunktival goblet hücreleri ve goblet olmayan hücrelere bağlanmıştır. Con A her iki türde goblet olmayan hücreler ile reaksiyon vermiştir. Keklik konjunktival goblet hücreleri Con A’ya karşı herhangi bir reaksiyon göstermezken; bıldırcın konjunktival goblet hücreleri Con A’ya karşı çok zayıf reaksiyon göstermiştir. UEA-I, keklikte herhangi bir hücre tipine karşı reaksiyon göstermezken; bıldırcın konjunktival goblet hücreleri ve goblet olmayan hücreler UEA-I ile reaksiyon vermiştir. Her iki türde konjunktival epitelyal yüzeyler Con A, UEA-I, PNA ve HPA ile reaksiyon vermiştir. Bununla birlikte, MAA ve BSA I-B4 her iki türde herhangi bir hücre ve/veya kısma bağlanmamıştır. Bu sonuçlar glikokonjugat komposizyonunun bu türlerin goblet ve goblet olmayan hücrelerinde farklı olabildiğini, ancak goblet ve goblet olmayan hücrelerin içeriklerinin salındığı konjunktival epitelyal yüzeylerde çok benzer olabildiğini göstermiştir.

Keywords

Keklik, Bıldırcın, Lektin Histokimyası, Glikokonjugat, Konjunktiva, Goblet hücresi

Lectin Histochemistry of the Glycoconjugates in Partridge and Quail Conjunctival Epithelia

Abstract

The aim of this study was to examine the distribution of the glycoconjugates in conjunctival epithelium of partridge (Alectoris chukar) and quail (Coturnix coturnix). Eyelid samples harvested from both species were subjected to routine tissue processing. Lectin histochemistry was applied to sections in order to demonstrate the expression of glycoconjugates. Six different HRP-conjugated lectins (Con A, UEA-I, PNA, HPA, MAA and BSA I-B4) were used for this purpose. The conjunctival epithelium in both partridge and quail consisted of goblet cells and nongoblet cells. Both PNA and HPA bound, to varying degrees, to the goblet cells and nongoblet cells in conjunctival epithelium of partridge and quail. Con A reacted with nongoblet cells in both species. While partridge conjunctival goblet cells showed no reaction to Con A, quail conjunctival goblet cells had a very weak reaction to Con A. UEA-I did not bind to any cells in partridge, however, quail conjunctival goblet cells and nongoblet cells could react to UEA-I. In both species, conjunctival epithelial surfaces reacted with Con A, UEA-I, PNA and HPA. However, MAA and BSA I-B4 did not bind any cell and/or part in both partridge and quail conjunctiva. The present data suggest that composition of glycoconjugates could be different between goblet cells and nongoblet cells of these species, but it could be very similar at conjunctival epithelial surfaces on which the content of the goblet cells and nongoblet cells is released.

Keywords

Partridge, Quail, Glycoconjugates, Conjunctiva, Lectin histochemistry, Goblet Cell

References

• Argüeso P, Tisdale A, Mandel U, Letko E, Foster CS, Gipson IK (2003). The cell-layer- and cell-type-specific distribution of GalNAc-transferases in the ocular surface epithelia is altered during keratinization. Invest Ophthalmol Vis Sci, 44, 86-92.
• Çinar K, Öztop M, Özkarasu B (2016). Glycoconjugate composition of ovine parotid glands elucidated by lectins. J Morphol Sci, 33(1), 8-13.
• Dartt DA, Kessler TL, Chung E, Zieske JD (1996). Vasoactive intestinal peptide-stimulated glycoconjugate secretion from conjunctival goblet cells. Exp Eye Res, 63, 27-34.
• Doughty MJ (2012). Goblet cells of the normal human bulbar conjunctiva and their assessment by impression cytology sampling. Ocul Surf, 10(3), 149-169.
• Gipson IK, Argüeso P (2003). Role of mucins in the function of the corneal and conjunctival epithelia. Int Rev Cytol, 231, 1-49.
• Hietanen J, Uusitalo M, Tarkkanen A, Kivela T (1995). Lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome. Br J Ophthalmol, 79, 467-472.
• Iwakiri N, Uehara F, Ohba N, Tsuyama S, Murata F (1997). Lectin histochemistry of the glycoconjugates in conjunctival goblet cells. J Jpn Ophthalmol Soc, 101, 83-86.
• Kawano K, Uehara F, Sameshima M, Ohba N (1984). Application of lectins for detection of goblet cell carbohydrates of the Human Conjunctiva. Exp Eye Res, 38, 439-447.
• Latkovic S (1991). Ultrastructural localization of lectin-binding sites on the surface of the guinea pig conjunctival epithelium. Graefe's Arch Clin Exp Ophthalmol, 229, 153-156.
• Lawton A (1998). Structure and function of the eyelids and conjunctiva. In: The Cornea, Kaufman H, Barron B, McDonald M (Ed), 51-61, Butterworth-Heinemann, Boston.
• Lis H, Sela B, Sachs L, Sharon N (1970). Specific inhibition by N-acetyI-D-galactosamine of the interaction between soybean agglutinin and animal cell surfaces. Biochim Biophys Acta, 211, 582-585.
• Liu H, Meagher CK, Moore CP, Phillips TE (2005). M Cells in the follicle-associated epithelium of the rabbit conjunctiva preferentially bind and translocate latex beads. Invest Ophthalmol Vis Sci, 46 (11), 4217-4223.
• Maeda S, Ishikawa M, Abe T, Sakuragi S (1998). Lectin cytochemistry of the rabbit conjunctiva and lacrimal sac. Jpn J Ophthalmol, 42, 443-449.
• Öztop M, Cinar K, Turk S (2018). Immunolocalization of natriuretic peptides and their receptors in goat (Capra hircus) heart. Biotech Histochem, 93(6), 389-404.
• Reid L, Clamp JR (1978). The biochemical and histochemical nomenclature of mucus. Br Med Bull, 34(1), 5-8.
• Ríos JD, Zoukhri D, Rawe IM, Hodges RR, Zieske JD, Dartt DA (1999). Immunolocalization of muscarinic and VIP receptor subtypes and their role in stimulating goblet cell secretion. Invest Ophthalmol Vis Sci, 40, 1102-1111.
• Ríos JD, Forde K, Diebold Y, Lightman J, Zieske JD, Dartt DA (2000). Development of conjunctival goblet cells and their neuroreceptor subtype expression. Invest Ophthalmol Vis Sci, 41, 2127-2137.
• Royle L, Matthews E, Corfield A, Berry M, Rudd PM, Dwek RA, Carrington SD (2008). Glycan structures of ocular surface mucins in man, rabbit and dog display species differences. Glycoconj J, 25, 763-773.
• Shatos MA, Ríos JD, Tepavcevic V, Kano H, Hodges R, Dartt DA (2001). Isolation, characterization and propagation of rat conjunctival goblet cells in vitro. Invest Ophthalmol Vis Sci, 42, 1455-1464.
• Spicer SS, Schulte BA (1992). Diversity of cell glycoconjugates shown histochemically: a perspective. J Histochem Cytochem, 40, 1-38.
• Stahl, JL, Cook EB, Barney NP, Graziano FM (2002). Pathophysiology of ocular allergy: The roles of conjunctival mast cells and epithelial cells. Curr Allergy Asthma Rep, 2, 332-339.
• Şeftalioğlu A, Tezel G, Tezel T, Alabay B (1993). Ultrastructural demonstration of Helix pomatia lectin-binding sites in goblet cells of human conjunctiva. J Islamic Acad Sci, 6(1), 46-51.
• Wells PA, DeSiena-Shaw C, Rice B, Foster CS (1988). Detection of ocular mucus in normal human conjunctiva and conjunctiva from patients with cicatricial pemphigoid using lectin probes and histochemical techniques. Exp Eye Res, 46(4), 485-497.
• Yanoff M, Cameron D (2011). Diseases of the Visual System. In: Goldman's Cecil Medicine, Lee G, Schafer AI (Ed), 2426-2442, Elsevier-Saunders, Philadelphia