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Direkt Embriyo Transferi: Azot Tankından Uterusa

Year 2021, Volume: 32 Issue: 2, 69 - 73, 31.07.2021
https://doi.org/10.36483/vanvetj.941855

Abstract

Sunulan çalışmanın amacı taşıyıcı düvelere yapılan direkt embriyo transferinde bazı parametrelerin gebelik oranları üzerine etkisini ortaya koymaktır. Çalışmanın hayvan materyalini 89 baş düve oluşturdu. Gebe kalmayanlara tekrar transfer uygulandığı için toplam 138 embriyo transferi yapıldı. Transferlerin 91’i direkt transfer, 47’si ise su banyosunda çözündürüldükten sonra 2 medyumda sırasıyla 5’er dk bekletildikten sonra, tekrar payetlenerek yapıldı. Östrus - ovulasyon zaman aralığı, ovulasyonun meydana geldiği ovaryum tarafı, ovulasyondan sonra transfer gününe kadar geçen zaman aralığı, embriyo pratisyenleri, direkt veya aşamalı çözündürülerek yapılan transferlerin gebelik oranı üzerine etkisi önemli bulunmadı (P>0.05). Embriyonun aşamasına göre değerlendirildiğinde morula, erken blastosist ve blastosistlerde gebelik oranları sırasıyla %64.3 (n=27), %44.3 (n=27) ve %74.3 (n=26) olarak tespit edildi. Morula ve blastosistlerin transferi sonrası gebelik oranları, erken blastosist aşamasında yapılanlara göre anlamlı derecede yüksek bulundu (P<0.05). Gebe düvelerde embriyonik ve fötal ölüme bağlı olarak %17.5 (n=14) kayıp yaşandı. Transfer edilen embriyoların morula ve blastosist aşamasında olması, transfer öncesi aktif bir korpus luteumun varlığının tespit edilmesi, direkt transferlerin hızlı yapılması, embriyonun bilinen pratisyenden temin edilmesi ve transferlerin aşamalı çözündürülerek yapılmasının gebelik oranlarını arttırabileceği düşünülmektedir.

Supporting Institution

Cumhurbaşkanlığı Strateji ve Bütçe Başkanlığı & Yüksek Öğretim Kurulu

Project Number

2017K12-41003-01

References

  • Alkan H, Karaşahin T, Dursun Ş, Satılmış F, Erdem H, Güler M (2020). Evaluation of the factors that affect the pregnancy rates during embryo transfer in beef heifers. Reprod Dom Anim, 55(4), 421-428.
  • Beal WE, Hinshaw RH, Whitman SS (1998). Evaluating embryo freezing method and the site of embryo deposition on pregnancy rate in bovine embryo transfer. Theriogenology, 1(49), 241.
  • Cerbito WA, Miyamoto A, Ootani M, Torres JF, Sato1 K (1994). Spatial distribution of progesterone in bovine uterus in relation to corpus luteum location. J Reprod Dev, 40, 35.
  • Curtis JL (2015). Cattle Embryo Transfer Procedure. I. Edition. K.S. Manhattan, USA
  • Demetrio DGB, Santos RM, Demetrio CGB, Vasconcelos JLM (2007). Factors affecting conception rates following artificial insemination or embryo transfer in lactating Holstein cows. J Dairy Sci, 90(11), 5073-5082.
  • Dochi O (2019). Direct transfer of frozen-thawed bovine embryos and its application in cattle reproduction management. J Reprod Dev, 65(5), 389-396.
  • Dochi O, Imai K, Takakura H (1995). Birth of calves after direct transfer of thawed bovine embryos stored frozen in ethylene glycol. Anim Reprod Sci, 38(3), 179-185.
  • Dursun Ş, Köse M, Kırbaş M, Bülbül B, Ümütlü S (2014). Etilen glikolle direkt transfer metoduna göre dondurulmuş sığır embriyolarının transferinde çözdürme-transfer aralığının gebelik oranı üzerine etkisi. Eurasian J Vet Sci, 30(2), 48-52.
  • Ergene O (2011). İneklerde embriyonik yaşamın desteklenmesine yönelik hormonal girişimler. Erciyes Üniv Vet Fak Derg, 8(2), 127-137.
  • Fair T, Lonergan P, Dinnyes A, Cottell DC, Hyttel P, Ward FA, Boland MP (2001). Ultrastructure of bovine blastocysts following cryopreservation: Effect of method of blastocyst production. Mol Reprod Dev, 58(2), 186-195.
  • Gadisa M, Furgasa W, Duguma M (2019). Review on Embryo Transfer and It’s Application in Animal Production. AJMS, 1(1), 4-12.
  • Gomez E, Carrocera S, Martin D, Perez-Janez JJ, Prendes J, Prendes JM, Vazquez A, Murillo A, Gimeno I, Munoz M (2020). Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium. Theriogenology, 146, 39-47.
  • Gordon I (2003). Laboratory production of cattle embryos. Persley GJ (Ed). Culturing and Evaluating the Early Bovine Embryo (pp. 112-302). Cromwell Press, Trowbridge.
  • Hasler JF, McCauley AD, Lathrop WF, Foote RH (1987). Effect of donor-recipient interactions on pregnancy rate in a large - scale bovine embryo transfer program. Theriogenology, 27(1), 139-168.
  • Hasler JF (2003). The current status and future of commercial embryo transfer in cattle. Anim Reprod Sci, 79(3-4), 245-264.
  • Hasler JF (2014). Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology, 81(1), 152–169.
  • Ismirandy A, Sonjaya H, Hasbi H (2020). The Outcome of in Vitro Embryo Transfer on Bali Cattle by Utilizing Fresh and Frozen Embryos. IJSBAR, 50(1), 200-206.
  • Kasai M (1996). Simple and efficient methods for vitrification of mammalian embryos. Anim Reprod Sci, 42(1-4), 67-75.
  • Kawarsky SJ, Hansen PJ, Stubbings RB, Basrur PK, King WA (1994). Influence of chromosomal make-up on growth rate of bovine embryos. BOR, 50(1), 88.
  • Kızıl HS, Akyol N, Karaşahin T, Satılmış M (2011). Etilen glikol ile direkt transfer metoduna göre dondurulan in vivo sığır embriyolarının transferi. Kafkas Univ Vet Fak Derg, 17(5), 721-724.
  • Massip A, Van Der Zwalmen P (1984). Direct transfer of frozen cow embryos in glycerol-sucrose. Vet Rec, 115(13), 327-328.
  • Mollo A, Lora M, Faustini M, Romagnoli S, Cairoli F (2007). Some factors affecting embryo transfer success in dairy cows. J Anim Vet Adv, 6(4), 496-499.
  • Nibart M, Humblot P (1997). Pregnancy rates following direct transfer of glycerol sucrose or ethylene glycol cryopreserved bovine embryos. Theriogenology, 47(1), 371.
  • Peterson AJ, McMillan WH, Thompson JG (2000). Various allantoic pathologies are associated with malformation of allantoic development of the IVP bovine embryo. In: Proceedings 14th International Congress on Animal Reproduction, Stockholm, Sweden.
  • Purcell SH, Beal WE, Gray KR (2005). Effect of a CIDR insert and flunixin meglumine, administered at the time of embryo transfer, on pregnancy rate and resynchronization of estrus in beef cattle. Theriogenology, 64(4), 867–878.
  • Haji SA (2020). Breed, parıty and frequency of collectıon ınfluences on qualıty and quantıty of oocytes obtaıned through ultrasound guıded transvagınal follıcular aspıratıon ın daıry cattle. M.Sc. Thesis, Addis Ababa University, College of Veterinary Medicine and Agriculture, Department of Clinical Studies, Bishoftu, Ethiopia.
  • Sağırkaya H (2009). Sığırlarda embriyo transfer uygulaması ve Türkiye açısından önemi. Uludag Üniv J Fac Vet Med, 28(2), 11-20.
  • Sanches BV, Lunardelli PA, Tannura JH, Cardoso BL, Pereira MHC, Gaitkoski D, Basso AC, Arnold DR, Seneda, MM (2016). A new direct transfer protocol for cryopreserved IVF embryos. Theriogenology, 85(6), 1147–1151.
  • Scenna FN, Hockett ME, Towns TM, Saxton AM, Rohrbach NR, Wehrman ME, Schrick FN (2005). Influence of a prostaglandin synthesis inhibitor administered at embryo transfer on pregnancy rates of recipient cows. Prostaglandins & Other Lipid Mediators, 78(1-4), 38–45.
  • Schneider HJ, Castelberry RS, Griffen JL (1980). Commercial aspects of bovine embryo transfer. Theriogenology, 13(1), 73-85.
  • Schrick FN, Inskeep EK, Butcher RL (1993). Pregnancy rates for embryos transferred from early postpartum beef cows into recipients with normal estrous cycles. BOR, 49(3), 617– 621.
  • Seidel GE (2007). New technologies for reproduction in cattle. In: Proceedings, Applied Reproductive Strategies in Beef Cattle, Billings, Montana.
  • Spell AR, Beal WE, Corah LR, Lamb GC (2001). Evaluating Recipient and Embryo Factors That Affect Pregnancy Rates of Embryo Transfer in Beef Cattle. Theriogenology, 56(2), 287-297.
  • Suzuki T, Yamamoto M, Ooe M, Sakata A, Matsuoka M, Nishikata Y, Okamoto K (1990). Effect of sucrose concentration used for one-step dilution upon in vitro and in vivo survival of bovine embryo refrigerated in glycerol and 1, 2- propanediol. Theriogenology, 34(6), 1051-1057.
  • Thompson JG, Peterson AJ (2000). Bovine embryo culture in vitro: New developments and post-transfer consequences. Hum Reprod, 15(5), 59–67.
  • Van Eerdenburg FJCM, Karthaus D, Taverne MAM, Mercis I, Szenci O (2002). The relationship between estrous behavioral score and time of ovulation in dairy cattle. J Dairy Sci, 85(5), 1150-1156.
  • Vanroose G, de Kruif A, Van Soom A (2000). Embryonic mortality and embryo-pathogen interactions. Anim Reprod Sci, 60(61), 131–143.
  • Viana J (2019). 2018 Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter, 36(4), 1-26.
  • Voelkel SA, Hu YX (1992). Direct transfer of frozen-thawed bovine embryos. Theriogenology, 37(1), 23-37. Willadsen S, Polge C, Rowson LEA (1978). The viability of deep-frozen cow embryos. J Reprod Fertil, 52(2), 391-393.
  • Willett EL, Black WG, Casida LE, Stone WH, Buckner PJ (1951). Successful transplantation of a fertilized bovine ovum. Science, 113(2931), 247.

Direct embryo transfer: From nitrogen tank to the uterus

Year 2021, Volume: 32 Issue: 2, 69 - 73, 31.07.2021
https://doi.org/10.36483/vanvetj.941855

Abstract

The aim of this study was to determine the effect of some parameters on pregnancy rates in direct embryo transfer to recipient heifers. Animal material of the study consisted of 89 heifers. A total of 138 embryos were transferred because the heifers that did not conceive were retransferred. Ninety one of the transfers were performed directly, 47 of them were performed after being thawed in the water bath and being held in 2 medium for 5 minutes. Estrus - ovulation time interval, the side of the ovary where ovulation occurred, the time interval from ovulation to the day of transfer, embryo practitioners, direct or gradually thawed transfers had no effect on the pregnancy rate (P> 0.05). When evaluated according to the stage of the embryo, pregnancy rates in morula, early blastocysts and blastocysts were 64.3% (n=27), 44.3% (n=27) and 74.3% (n=26), respectively. Pregnancy rate after transfer of blastocysts and morula was significantly higher than those of the early blastocyst stage (P<0.05). There was a 17.5% (n=14) loss due to embryonic and fetal death in pregnant heifers. It is thought that transferring embryos at the morula and blastocyst stage, the presence of an active corpus luteum before the transfer, rapid direct transfers, the procurement of the embryo from the known practitioner and the gradually thawing of the transfers can increase the pregnancy rates.

Project Number

2017K12-41003-01

References

  • Alkan H, Karaşahin T, Dursun Ş, Satılmış F, Erdem H, Güler M (2020). Evaluation of the factors that affect the pregnancy rates during embryo transfer in beef heifers. Reprod Dom Anim, 55(4), 421-428.
  • Beal WE, Hinshaw RH, Whitman SS (1998). Evaluating embryo freezing method and the site of embryo deposition on pregnancy rate in bovine embryo transfer. Theriogenology, 1(49), 241.
  • Cerbito WA, Miyamoto A, Ootani M, Torres JF, Sato1 K (1994). Spatial distribution of progesterone in bovine uterus in relation to corpus luteum location. J Reprod Dev, 40, 35.
  • Curtis JL (2015). Cattle Embryo Transfer Procedure. I. Edition. K.S. Manhattan, USA
  • Demetrio DGB, Santos RM, Demetrio CGB, Vasconcelos JLM (2007). Factors affecting conception rates following artificial insemination or embryo transfer in lactating Holstein cows. J Dairy Sci, 90(11), 5073-5082.
  • Dochi O (2019). Direct transfer of frozen-thawed bovine embryos and its application in cattle reproduction management. J Reprod Dev, 65(5), 389-396.
  • Dochi O, Imai K, Takakura H (1995). Birth of calves after direct transfer of thawed bovine embryos stored frozen in ethylene glycol. Anim Reprod Sci, 38(3), 179-185.
  • Dursun Ş, Köse M, Kırbaş M, Bülbül B, Ümütlü S (2014). Etilen glikolle direkt transfer metoduna göre dondurulmuş sığır embriyolarının transferinde çözdürme-transfer aralığının gebelik oranı üzerine etkisi. Eurasian J Vet Sci, 30(2), 48-52.
  • Ergene O (2011). İneklerde embriyonik yaşamın desteklenmesine yönelik hormonal girişimler. Erciyes Üniv Vet Fak Derg, 8(2), 127-137.
  • Fair T, Lonergan P, Dinnyes A, Cottell DC, Hyttel P, Ward FA, Boland MP (2001). Ultrastructure of bovine blastocysts following cryopreservation: Effect of method of blastocyst production. Mol Reprod Dev, 58(2), 186-195.
  • Gadisa M, Furgasa W, Duguma M (2019). Review on Embryo Transfer and It’s Application in Animal Production. AJMS, 1(1), 4-12.
  • Gomez E, Carrocera S, Martin D, Perez-Janez JJ, Prendes J, Prendes JM, Vazquez A, Murillo A, Gimeno I, Munoz M (2020). Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium. Theriogenology, 146, 39-47.
  • Gordon I (2003). Laboratory production of cattle embryos. Persley GJ (Ed). Culturing and Evaluating the Early Bovine Embryo (pp. 112-302). Cromwell Press, Trowbridge.
  • Hasler JF, McCauley AD, Lathrop WF, Foote RH (1987). Effect of donor-recipient interactions on pregnancy rate in a large - scale bovine embryo transfer program. Theriogenology, 27(1), 139-168.
  • Hasler JF (2003). The current status and future of commercial embryo transfer in cattle. Anim Reprod Sci, 79(3-4), 245-264.
  • Hasler JF (2014). Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology, 81(1), 152–169.
  • Ismirandy A, Sonjaya H, Hasbi H (2020). The Outcome of in Vitro Embryo Transfer on Bali Cattle by Utilizing Fresh and Frozen Embryos. IJSBAR, 50(1), 200-206.
  • Kasai M (1996). Simple and efficient methods for vitrification of mammalian embryos. Anim Reprod Sci, 42(1-4), 67-75.
  • Kawarsky SJ, Hansen PJ, Stubbings RB, Basrur PK, King WA (1994). Influence of chromosomal make-up on growth rate of bovine embryos. BOR, 50(1), 88.
  • Kızıl HS, Akyol N, Karaşahin T, Satılmış M (2011). Etilen glikol ile direkt transfer metoduna göre dondurulan in vivo sığır embriyolarının transferi. Kafkas Univ Vet Fak Derg, 17(5), 721-724.
  • Massip A, Van Der Zwalmen P (1984). Direct transfer of frozen cow embryos in glycerol-sucrose. Vet Rec, 115(13), 327-328.
  • Mollo A, Lora M, Faustini M, Romagnoli S, Cairoli F (2007). Some factors affecting embryo transfer success in dairy cows. J Anim Vet Adv, 6(4), 496-499.
  • Nibart M, Humblot P (1997). Pregnancy rates following direct transfer of glycerol sucrose or ethylene glycol cryopreserved bovine embryos. Theriogenology, 47(1), 371.
  • Peterson AJ, McMillan WH, Thompson JG (2000). Various allantoic pathologies are associated with malformation of allantoic development of the IVP bovine embryo. In: Proceedings 14th International Congress on Animal Reproduction, Stockholm, Sweden.
  • Purcell SH, Beal WE, Gray KR (2005). Effect of a CIDR insert and flunixin meglumine, administered at the time of embryo transfer, on pregnancy rate and resynchronization of estrus in beef cattle. Theriogenology, 64(4), 867–878.
  • Haji SA (2020). Breed, parıty and frequency of collectıon ınfluences on qualıty and quantıty of oocytes obtaıned through ultrasound guıded transvagınal follıcular aspıratıon ın daıry cattle. M.Sc. Thesis, Addis Ababa University, College of Veterinary Medicine and Agriculture, Department of Clinical Studies, Bishoftu, Ethiopia.
  • Sağırkaya H (2009). Sığırlarda embriyo transfer uygulaması ve Türkiye açısından önemi. Uludag Üniv J Fac Vet Med, 28(2), 11-20.
  • Sanches BV, Lunardelli PA, Tannura JH, Cardoso BL, Pereira MHC, Gaitkoski D, Basso AC, Arnold DR, Seneda, MM (2016). A new direct transfer protocol for cryopreserved IVF embryos. Theriogenology, 85(6), 1147–1151.
  • Scenna FN, Hockett ME, Towns TM, Saxton AM, Rohrbach NR, Wehrman ME, Schrick FN (2005). Influence of a prostaglandin synthesis inhibitor administered at embryo transfer on pregnancy rates of recipient cows. Prostaglandins & Other Lipid Mediators, 78(1-4), 38–45.
  • Schneider HJ, Castelberry RS, Griffen JL (1980). Commercial aspects of bovine embryo transfer. Theriogenology, 13(1), 73-85.
  • Schrick FN, Inskeep EK, Butcher RL (1993). Pregnancy rates for embryos transferred from early postpartum beef cows into recipients with normal estrous cycles. BOR, 49(3), 617– 621.
  • Seidel GE (2007). New technologies for reproduction in cattle. In: Proceedings, Applied Reproductive Strategies in Beef Cattle, Billings, Montana.
  • Spell AR, Beal WE, Corah LR, Lamb GC (2001). Evaluating Recipient and Embryo Factors That Affect Pregnancy Rates of Embryo Transfer in Beef Cattle. Theriogenology, 56(2), 287-297.
  • Suzuki T, Yamamoto M, Ooe M, Sakata A, Matsuoka M, Nishikata Y, Okamoto K (1990). Effect of sucrose concentration used for one-step dilution upon in vitro and in vivo survival of bovine embryo refrigerated in glycerol and 1, 2- propanediol. Theriogenology, 34(6), 1051-1057.
  • Thompson JG, Peterson AJ (2000). Bovine embryo culture in vitro: New developments and post-transfer consequences. Hum Reprod, 15(5), 59–67.
  • Van Eerdenburg FJCM, Karthaus D, Taverne MAM, Mercis I, Szenci O (2002). The relationship between estrous behavioral score and time of ovulation in dairy cattle. J Dairy Sci, 85(5), 1150-1156.
  • Vanroose G, de Kruif A, Van Soom A (2000). Embryonic mortality and embryo-pathogen interactions. Anim Reprod Sci, 60(61), 131–143.
  • Viana J (2019). 2018 Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter, 36(4), 1-26.
  • Voelkel SA, Hu YX (1992). Direct transfer of frozen-thawed bovine embryos. Theriogenology, 37(1), 23-37. Willadsen S, Polge C, Rowson LEA (1978). The viability of deep-frozen cow embryos. J Reprod Fertil, 52(2), 391-393.
  • Willett EL, Black WG, Casida LE, Stone WH, Buckner PJ (1951). Successful transplantation of a fertilized bovine ovum. Science, 113(2931), 247.
There are 40 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Araştırma Makaleleri
Authors

Şöhret Güler This is me 0000-0003-1334-290X

Mehmet Yıldız 0000-0001-5523-7433

Yunus Çetin 0000-0002-5402-9429

Project Number 2017K12-41003-01
Publication Date July 31, 2021
Submission Date May 27, 2021
Acceptance Date July 13, 2021
Published in Issue Year 2021 Volume: 32 Issue: 2

Cite

APA Güler, Ş., Yıldız, M., & Çetin, Y. (2021). Direkt Embriyo Transferi: Azot Tankından Uterusa. Van Veterinary Journal, 32(2), 69-73. https://doi.org/10.36483/vanvetj.941855
AMA Güler Ş, Yıldız M, Çetin Y. Direkt Embriyo Transferi: Azot Tankından Uterusa. Van Vet J. July 2021;32(2):69-73. doi:10.36483/vanvetj.941855
Chicago Güler, Şöhret, Mehmet Yıldız, and Yunus Çetin. “Direkt Embriyo Transferi: Azot Tankından Uterusa”. Van Veterinary Journal 32, no. 2 (July 2021): 69-73. https://doi.org/10.36483/vanvetj.941855.
EndNote Güler Ş, Yıldız M, Çetin Y (July 1, 2021) Direkt Embriyo Transferi: Azot Tankından Uterusa. Van Veterinary Journal 32 2 69–73.
IEEE Ş. Güler, M. Yıldız, and Y. Çetin, “Direkt Embriyo Transferi: Azot Tankından Uterusa”, Van Vet J, vol. 32, no. 2, pp. 69–73, 2021, doi: 10.36483/vanvetj.941855.
ISNAD Güler, Şöhret et al. “Direkt Embriyo Transferi: Azot Tankından Uterusa”. Van Veterinary Journal 32/2 (July 2021), 69-73. https://doi.org/10.36483/vanvetj.941855.
JAMA Güler Ş, Yıldız M, Çetin Y. Direkt Embriyo Transferi: Azot Tankından Uterusa. Van Vet J. 2021;32:69–73.
MLA Güler, Şöhret et al. “Direkt Embriyo Transferi: Azot Tankından Uterusa”. Van Veterinary Journal, vol. 32, no. 2, 2021, pp. 69-73, doi:10.36483/vanvetj.941855.
Vancouver Güler Ş, Yıldız M, Çetin Y. Direkt Embriyo Transferi: Azot Tankından Uterusa. Van Vet J. 2021;32(2):69-73.

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