Inovatif Gastroentero-Dermatoloji Kapsamında Muhtelif Yöntem Geliştirme I: Lactobacillus plantarum ve Lactobacillus paracasei ile Probiyotik Eneması Atopik Dermatitli Köpeklerde Anti-Pruritik Etkinlik Sağlar Mı?

Year 2021, Volume: 32 Issue: 2, 74 - 81, 31.07.2021

Kerem Ural , Songül Erdoğan , Canberk Balıkçı , Hasan Erdoğan , Şükran Gözde İçaçan

https://doi.org/10.36483/vanvetj.941978

Cited By: 1

Abstract

İnsanlarda önemli bir dermatolojik problem olan atopik dermatit (Ad) köpekler içinde benzer durum teşkil etmektedir. Ad’ li hastalarda sızıntılı bağırsak ilişkili intestinal permeabilite artışı ve bağırsak mikrobiyatasının bozulmasının hastalıkta rol oynadığı bilinmesine rağmen hala bu konuda gerek tanı gerekse sağaltım anlamında bilinmeyenler mevcuttur. Bu çalışmada Ad ile bağırsak mikrobiyatası arasındaki ilişki baz alınarak; 1) en önde gelen semptom olan kaşıntının sağaltımında Lactobacillus plantarum ve Lactbacillus paracasei ile probiyotik enemasının kullanılıp kullanılamayacağının belirlenmesi, 2) rol model sağaltım için umut vadeden yeni, güncel probiyoterapi seçeneklerinden birisinin oral uygulamadaki emilimdeki sorunların aksine rektal enema, diğer adıyla proktolizis, yolu ile uygulanmasının değerlendirilmesi amaçlanmıştır. Çalışmamıza baskın klinik bulgu olarak kaşıntısı bulunan toplamda 12 farklı yaş, ırk ve her iki cinsiyetteki Ad’li köpek dahil edilmiştir. Daha önce sağaltım geçmişi olmayan, Favrot kriterleri ve atopi ile uyumlu klinik bulgulara eşlik eden alerjen-spesifik IgE düzeyinde artış şekillenmiş olgular (sağaltım öncesi), CADESI-04 skorları ve klinik bulgular eşliğinde belirlendikten sonra rektal enema yolu ile nutrasötiklerle desteklenmiş Lactobacillus plantarum ve Lactbacillus paracasei ile probiyotik eneması foley kateteri ya da rektal kateter vasıtası ile rektumdan 10-15 cm ileriye uygulandı. Ad’ li ve rektal enema ile probiyoterapi uygulanan 12 olguda kaşıntı 0 ila 10. günler arasında belirgin şekilde kesildi. Sonuç olarak antipruritik laktik asit bakterileri içerisinde değerlendirilebilecek olan L. plantarum ve L. paracasei suşlarının, bu araştırmada önerilen ve uygulanan dozda/sürede, atopik dermatitli köpeklerde hem klinik iyileşme hem de kaşıntının giderilmesi amacıyla kullanılabileceği görülmüştür.

Keywords

atopik dermatit, kaşıntı, köpek, probiyoterapi

Development of Various Methods in Innovative Gastroentero-Dermatology I: Does Probiotic Enema with Lactobacillus plantarum and Lactobacillus paracasei Provide Anti-Pruritic Efficacy in Dogs with Atopic Dermatitis?

Abstract

Atopic dermatitis (Ad), an important dermatological problem for humans, similar to dogs. Although it is known that leaky gut-related increase in intestinal permeability and deterioration of the intestinal microbiota play a role in the disease, there are still unknowns regarding in term of both diagnosis and treatment. In this study, based on the relationship between atopic dermatitis and intestinal microbiota; 1) determination of whether Lactobacillus plantarum and Lactbacillus paracasei and probiotic enema can be used in the treatment of itching, which is the leading symptom, 2) aimed to evaluate the administration of one of the new, up-to-date probiotherapy options that are promising for role model treatment by rectal enema, also known as proctolysis, in contrast to problems of absorption in oral administration. In our study, a total of 12 dogs with Ad as different ages, breeds, and both sexes were included. Cases which had no previous treatment history, showed clinical findings compatible with Favrot criteria and atopy accompanying with increasing allergen-specific IgE level, were determined in the light of allergen-specific IgE levels (before treatment), CADESI-04 scores and clinical findings, and then rectal enema with nutraceuticals. Probiotic enema with Lactobacillus plantarum and Lactbacillus paracasei was applied 10-15 cm forward from the rectum via foley catheter or rectal catheter. Pruritus of 12 dogs with Ad applied probiotherapy by rectal enema were ceased significantly between 0 and 10 days. In conclusion, it has been observed that L. plantarum and L. paracasei strains, which can be evaluated in antipruritic lactic acid bacteria, can be used for both clinical improvement and relief of itching in dogs with atopic dermatitis at the recommended and applied dose/time in this study.

Keywords

atopic dermatitis, dog, probiotherapy, pruritis

References

• Amit-Romach E, Uni Z, Friedman M, Aizenberg I, Berkovich Z, et al. (2015). A new mode of probiotic therapy: Specific targeting. J Funct Foods, 16, 386-392.
• Cho J, Lee D, Yang C, Jeon J, Kim J, et al. (2006). Microbial population dynamics of kimchi, a fermented cabbage product. FEMS Microbiol Lett, 257, 262–267.
• Cosgrove SB, Wren JA, Cleaver DM, Martin DD, Walsh KF, et al. (2013). Efficacy and safety of oclacitinib for the control of pruritus and associated skin lesions in dogs with canine allergic dermatitis. Vet Dermatol, 24(5), 479-e114.
• Craig JM (2016). Atopic dermatitis and the intestinal microbiota in humans and dogs. Vet Med Sci, 2(2), 95-105.
• Cukrowska B, Bierła JB, Zakrzewska M, Klukowski M, Maciorkowska E (2020). The relationship between the infant gut microbiota and allergy. The role of bifidobacterium breve and prebiotic oligosaccharides in the activation of anti-allergic mechanisms in early life. Nutrients, 12(4), 946.
• D’Incà R, Barollo M, Scarpa M, Grillo AR, Brun P, et al. (2011). Rectal administration of Lactobacillus casei DG modifies flora composition and Toll-like receptor expression in colonic mucosa of patients with mild ulcerative colitis. Dig Dis Sci, 56(4), 1178-1187.
• Drago L, Iemoli E, Rodighiero V, Nicola L, De Vecchi E, et al. (2011). Effects of Lactobacillus salivarius LS01 (DSM 22775) treatment on adult atopic dermatitis: a randomized placebo-controlled study. Int J Immunopathol Pharmacol, 24(4), 1037-1048.
• Favrot C, Steffan J, Seewald W, Picco F (2010). A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol, 21(1), 23-31.
• Franciosi E, Carafa I, Nardin T, Schiavon S, Poznanski E, et al. (2015). Biodiversity and γ-aminobutyric acid production by lactic acid bacteria isolated from traditional alpine raw cow’s milk cheeses. Biomed Res Int, 625740, 1-11.
• Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, et al. (2017). Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol, 14(8), 491.
• Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, et al. (2014). The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol, 11(8), 506-514.
• Holgate ST (1999). The epidemic of allergy and asthma. Nature, 402, 182–184.
• Hong HJ, Kim E, Cho D, Kim TS (2010). Differential suppression of heat-killed lactobacilli isolated from kimchi, a Korean traditional food, on airway hyper-responsiveness in mice. J Clin Immunol,, 30(3), 449-458.
• Iemoli E, Trabattoni D, Parisotto S, Borgonovo L, Toscano M, et al. (2012). Probiotics reduce gut microbial translocation and improve adult atopic dermatitis. J Clin Gastroenterol, 46, S33-S40.
• Jang SE, Hyun YJ, Trinh HT, Han MJ, Kim DH (2011). Anti-scratching behavioral effect of Lactobacillus plantarum PM008 isolated from kimchi in mice. Immunopharmacol Immunotoxicol, 33(3), 539-544.
• Jugan MC, Rudinsky AJ, Parker VJ, Gilor C (2017). Use of probiotics in small animal veterinary medicine. J Am Vet Med Assoc, 250(5), 519-528.
• Khan A, Hallowell GD, Underwood C, van Eps AW (2019). Continuous fluid infusion per rectum compared with intravenous and nasogastric fluid administration in horses. Equine Vet J, 51(6), 767-773.
• Kim H, Jeon B, Kim WJ, Chung DK (2020a). Effect of paraprobiotic prepared from Kimchi-derived Lactobacillus plantarum K8 on skin moisturizing activity in human keratinocyte. J Funct Foods, 75, 104244.
• Kim WK, Jang YJ, Han DH, Jeon K, Lee C, et al. (2020b). Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota. Gut Microbes, 12(1), 1819156.
• Kim HW, Hong R, Choi EY, Yu K, Kim N, et al. (2018). A probiotic mixture regulates T cell balance and reduces atopic dermatitis symptoms in mice. Front Microbiol, 9, 2414.
• Lee DE, Huh CS, Ra J, Choi ID, Jeong JW, Kim SH, Ahn YT, et al. (2015). Clinical evidence of effects of Lactobacillus plantarum HY7714 on skin aging: a randomized, double blind, placebo-controlled study. J Microbiol Biotechnol, 25(12), 2160-2168.
• Lee SY, Lee E, Park YM, Hong SJ (2018). Microbiome in the gut-skin axis in atopic dermatitis. Allergy Asthma Immunol Res, 10(4), 354.
• Massimini M, Dalle Vedove E, Bachetti B, Di Pierro F, Ribecco C, et al. (2021). Polyphenols and Cannabidiol Modulate Transcriptional Regulation of Th1/Th2 Inflammatory Genes Related to Canine Atopic Dermatitis. Front Vet Sci, 8, 146.
• Matei MC, Buza V, Neagu D, Popovici CP, Szakacs AR, et al. (2020). Comparative ionogram assessment before and after probiotic treatment for healthy dogs and dogs with apparent dysbiosis. Lucr. ştiinţ. - Inst. Agron. "Nicolae Bălcescu", Ser. C Zooteh med Vet, 63(1), 2020.
• Matsumoto M, Ebata T, Hirooka J, Hosoya R, Inoue N, et al. (2014). Antipruritic effects of the probiotic strain LKM512 in adults with atopic dermatitis. Ann Allergy Asthma Immunol, 113(2), 209-216.
• Matthes H, Krummenerl T, Giensch M, Wolff C, Schulze J (2010). Clinical trial: probiotic treatment of acute distal ulcerative colitis with rectally administered Escherichia coli Nissle 1917 (EcN). BMC Complement Altern Med, 10(1), 1-8.
• Moens F, Van den Abbeele P, Basit AW, Dodoo C, Chatterjee R, et al. (2019). A four-strain probiotic exerts positive immunomodulatory effects by enhancing colonic butyrate production in vitro. Int J Pharm, 555, 1-10.
• Mueller RS, Burrows A, Tsohalis J (1999). Comparison of intradermal testing and serum testing for allergen‐specific IgE using monoclonal IgE antibodies in 84 atopic dogs. Aust Vet J, 77(5), 290-294. Penttila IA (2010). Milk-derived transforming growth factor-β and the infant immune response. J Pediatr, 156(2), 21-25.
• Prakoeswa CRS, Herwanto N, Prameswari R, Astari L, Sawitri S, et al. (2017). Lactobacillus plantarum IS-10506 supplementation reduced SCORAD in children with atopic dermatitis. Benef Microbes, 8(5), 833-840.
• Prakoeswa CRS, Bonita L, Karim A, Herwanto N, Umborowati MA, et al. (2020). Beneficial effect of Lactobacillus plantarum IS-10506 supplementation in adults with atopic dermatitis: a randomized controlled trial. J Dermatolog Treat, 1-8.
• Rather IA, Choi KH, Bajpai VK, Park YH (2015). Antiviral mode of action of Lactobacillus plantarum YML009 on Influenza virus H1N1. Bangladesh J Pharmacol, 10(2), 475-82.
• Roessler A, Friedrich U, Vogelsang H, Bauer A, Kaatz M, et al. (2008). The immune system in healthy adults and patients with atopic dermatitis seems to be affected differently by a probiotic intervention. Clin Exp Allergy, 38(1), 93-102.
• Santoro D, Hoffmann AR (2016). Canine and human atopic dermatitis: two faces of the same host-microbe interaction. J Invest Dermatol, 136(6), 1087-1089.
• Sirichokchatchawan W, Pupa P, Praechansri P, Am-In N, Tanasupawat S, et al. (2018). Autochthonous lactic acid bacteria isolated from pig faeces in Thailand show probiotic properties and antibacterial activity against enteric pathogenic bacteria. Microb Pathog, 119, 208-215.
• Ural K (2016). Farklı dermatolojik bozukluklarda ayırıcı tanı: ipucubeşlisi. Çağrılı ve sözlü tebliğ. 11. Küçük Hayvan Veteriner Hekimleri Derneği Sürekli Eğitim Kongresi, İstanbul, Türkiye.
• Ural K (2017a). 360 degree Veterinary Dermatology Erşan Kuneri aganist Sean Connery (probiotic therapy). II. International Congress on Advances in Veterinary Sciences Technics. Skopje, Macedonia.
• Ural K (2017b). Kedi ve köpeklerde dermatoloji çalıştayı: güncel ve pratik yaklaşımlar. 12. Küçük hayvan Veteriner Hekimleri Derneği Sürekli Eğitim Kongresi, İstanbul, Turkey.
• Ural K, Erdoğan H, Gültekin M (2018). Allergen specific IgE determination by in vitro allergy test in head and facial feline dermatitis: A pilot study. Vet J Ankara Univ, 65, 379-386.
• Ural K (2019a). Veteriner İç Hastalıkları Günleri-Mikrobiyota diyalogları 08.09.2019 (tam gün sunum) Aydın. Veteriner İç Hastalıkları Akademisi ve Felin Dermatoloji Grubu organizasyonu, Aydın, Türkiye.
• Ural K (2019b). Küçük hayvan hekimliğinde dermatoloji alanında yeni dönem: Bağırsak-beyin-deri ekseni-I ve II. Uluslararası Vetexpo Veteriner Bilimleri Kongresi Sözlü sunumları. İstanbul, Türkiye.
• Ural K (2020). Veteriner İç Hastalıklarında Probiyotikler: Probiyotik Kullanımı rehber Kitabı ve Olgu Atlası. 1. Baskı. Atalay konf ve Matb, Ankara.
• Ural K, Erdoğan S, Erdoğan H, Gültekin M, Gül G, Türk E, Arslan N, ve ark. (2020a). Köpeklerde Atopinin İyi, Kötü ve Çirkin Yönleri: Çoklu Olgu Serisi ile Retrospektif Çalışma. Turkiye Klinikleri J Vet Sci, 11(1),18-29.
• Ural K, Gültekin M, Erdoğan H, Erdoğan S, Gül G, Türk E ve ark. (2020b). Kısa Dönem Değişmeli Takvim Probiyotik Sağaltımıyla Atopik Dermatitli Köpeklerde Kaşıntı Giderilebilir mi? Turkiye Klinikleri J Vet Sci, 11(1), 1-8.
• Refeld A, Bogdanova A, Prazdnova E, Beskopylny A, Olshevskaya A, et al. (2020). Immunobiotics mechanisms of action and prospects of use in veterinary medicine. E3S Web of Conferences; Innovative Technologies in Science and EducationRostov-On-Don, Russia.
• Yoshida Y, Seki T, Matsunaka H, Watanabe T, Shindo M, et al. (2010). Clinical effects of probiotic Bifidobacterium breve supplementation in adult patients with atopic dermatitis. Yonago Acta Med, 53(2), 37-45.
• Zhuang K, Jiang Y, Feng X, Li L, Dang F, et al. (2018). Transcriptomic response to GABA-producing Lactobacillus plantarum CGMCC 1.2437 T induced by L-MSG. PloS One, 13(6), e0199021.