Bisphenol A'nın Rat Sindirim Kanalındaki Mast Hücrelerinin Dağılımı ve Heterojenitesi Üzerine Etkileri

Year 2022, Volume: 33 Issue: 3, 122 - 129, 29.11.2022

Halil Cumhur Yılmaz , Mecit Yörük

https://doi.org/10.36483/vanvetj.1173575

Cited By: 2

Abstract

Bisphenol A (BPA) dünya genelinde plastik ürünlerinde çok yaygın olarak kullanılan kimyasal bir bileşendir. Bu çalışmanın amacı insanların gündelik hayatlarında oldukça sık olarak maruz kaldığı BPA’nın mast hücrelerinin heterojenite ve dağılımı üzerine etkilerinin araştırılmasıdır. Çalışma için 24 adet erkek Wistar Albino sıçan 4 gruba (Kontrol, Sham, 25mg/kg ve 50 mg/kg) ayrıldı. Bu grupların hepsine günlük standart diyet uygulanırken sham grubuna mısır yağı, diğer iki gruba ise (25mg/kg ve 50mg/kg) mısır yağı içerisinde çözündürülmüş olarak BPA 30 gün süre ile gavaj yoluyla verildi. İnhalasyon anestezik ile uyutulan deney hayvanlarından alınan sindirim kanalı doku örnekleri Basic Lead Acetate (BLA) ve Carnoy tespit solüsyonları kullanılarak tespit edildi. Ardından rutin doku takibi yapılarak paraplast ile bloklandılar. Bloklardan alınan 6 µm kalınlığındaki kesitler toluidine blue (TB) ve alcian blue-safranin O 8GX (AB/SO) kombine boyaları kullanılarak boyandı. TB ile boyanan kesitlerde mast hücrelerinin sayımı ve istatistiksel analizleri yapıldı. Analiz sonuçlarına göre BLA tespit solüsyonunda Carnoy tespitine oranla daha fazla sayıda mast hücresi gözlemlenirken, BPA uygulanan gruplarda mast hücre sayılarındaki artış istatistiksel olarak anlamlı bulundu (p<0.05). AB-SO 8GX kombine boyama sonuçlarında ise sindirim kanalının incelenen neredeyse her kesitinde AB (+), S (+) ve mix (+) mast hücrelerine rastlandı. Ancak S (+) ve mix (+) Mast hücrelerinin sayıları genel olarak bakıldığında AB (+) mast hücrelerine oranla oldukça düşüktü. Yine bu boyama metodu neticesinde BPA uygulanan gruplar da dahil olmak üzere yapılan semi kantitatif değerlendirmelerde bir fark tespit edilemedi. Sonuç olarak BPA’nın mast hücrelerinin heterojenitesine bir etkisi istatistiksel olarak tespit edilemese de dağılımlarına etkisi olduğu gözlendi. Bundan dolayı daha ileri çalışmaların yapılması gerektiği düşünülmektedir.

Keywords

Mast hücresi, Bisphenol A, Gastrointestinal kanal, Heterojenite

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

TDK-2019-8358

The Effects of Bisphenol A on the Distribution and Heterogeneity of Mast Cells in Rat Digestive Tract

Abstract

Bisphenol A (BPA) is a chemical component used in plastic products around the world. This study aims to examine the effects of these chemical compounds to which humans are frequently exposed in everyday life, on the heterogeneity and distribution of mast cells in the gastrointestinal system. For the study, 24 male Wistar albino rats were divided into 4 groups (control, sham, 25 mg/kg, and 50 mg/kg BPA treated). BPA was dissolved in corn oil and administration was done by oral gavage for thirty days. Gastrointestinal tissue samples taken from animals anesthetized with inhalation anesthesia were fixed using BLA (Basic Lead Acetate) and Carnoy fixation. Then, following routine tissue follow-up, they were blocked with Paraplast. Sections (6 µm) taken from the blocks were stained using Toluidine blue (TB) and Alcian blue-safranine O 8GX (AB-SO) combined dyes. Counting and statistical analysis of the mast cells in the TB-stained sections were performed. According to the results of the analysis, a higher number of mast cells was observed in the BLA fixation solution, while the increase in the number of mast cells was statistically significant in the groups treated with BPA (p<0.05). In the combined AB-SO 8GX staining results, AB (+), S (+), and Mix (+) mastocytes were found in almost all sections of the digestive tract. As a result of this staining process, again, no difference was found in the semi-quantitative evaluations performed including the groups treated with BPA. Although BPA does not affect the heterogeneity of mast cells, it does affect their distribution. Therefore, it is assumed that further studies will need to be carried out.

Keywords

Mast cell, Bisphenol A, Gastrointestinal tract, Heterogeneity

Project Number

TDK-2019-8358

References

• Akin C (2017). Mast cell activation syndromes. J Allergy Clin Immunol, 140 (2), 349–355.
• Albert-Bayo M, Paracuellos I, González-Castro AM et al. (2019). Intestinal mucosal mast cells: key modulators of barrier function and homeostasis. Cells, 8 (2), 135.
• Aydemir I, Kum Ş & Tuğlu M İ (2018). Histological investigations on the thymus of male rats prenatally exposed to bisphenol A. Chemosphere, 206, 1–8.
• Bancroft JD and Cook HC (1994) Manual of histological techniques and their diagnostic application. 2. th ed. Edinburgh, Churchill Livingstone, New York.
• Becker AB, Chung KF, McDonald DM, et al. (1985). Mast cell heterogeneity in dog skin. Anat Rec, 213 (4), 477–480.
• Bodin J, Bølling AK, Becher R, et al. (2014). Transmaternal bisphenol-a exposure accelerates diabetes type 1 development in nod mice. Toxicol Sci, 137 (2), 311–323.
• Braniste V, Jouault A, Gaultier E, et al. (2010). Impact of oral bisphenol A at reference doses on intestinal barrier function and sex differences after perinatal exposure in rats. PNAS, 107 (1), 448–453.
• Enerback L (1966a) Mast cell in rat gastrointestinal mucosa 1. Effects of Fixations. Acta Path et Microbiol Scandinav, 66 (3), 289-302.
• Enerback L (1966b) Mast cell in rat gastrointestinal mucosa 2. Dye-Binding and metachromatic properties. Acta Path et Microbiol Scandinav, 66 (3), 303-312.
• Eren Ü, Güzel N, Türkütanıt S, Durukan A, Ergüldürenler Ş, Kara ME (2000). Köpeklerde barsak mukozasinda mast hücreleri. Ankara Üniv Vet Fak Derg, 47, 125–134.
• Ergün L (2016) Connective Tissue. Özer A, editör. Temel Histoloji. (3rd ed). Ankara: Dora Publishing.
• Ertuğrul T, Çi̇Ftci̇ G, Tütüncü Ş (2017). Formaldehite maruz kalmış ratlarda böbrek dokusunda mast hücrelerinin dağılımı ve heterojenitesi. Vet Hek Der Derg, 88 (2), 26-37.
• Ertuğrul T and Kurtdede N (2017). Sığır Koyun ve Keçilerin Üriner Sistem Mast Hücreleri Üzerinde Histolojik Çalışmalar. Lalahan Hay Araşt Enst Derg, 57 (1), 32-38.
• Ertugrul T, Tutuncu S, Kabak M, Onuk B (2018). The distribution and heterogeneity of mast cells in tongue from five different avian species. Anat Histol Embryol, 47 (4), 306–312.
• Frossi B, Mion F, Sibilano R, Danelli L, Pucillo CEM (2018). Is it time for a new classification of mast cells? What do we know about mast cell heterogeneity? Immunol Rev, 282 (1), 35–46.
• Karaca T and Yörük M (2004). A Morphological and histometrical study on distribution and heterogeneity of mast cells of chicken’s and quail’s digestive tract. Van Vet J, 15 (1-2), 115-121.
• Kurtdede N, Yörük M. (1995) Tavuk ve bıldırcın derisinde mast hücrelerinin morfolojik ve histometrik incelenmesi. Ankara Üniv Vet Fak Derg, 42, 77-83.
• Menard S, Guzylack-Piriou L, Lencina C, Leveque M, Naturel M, Sekkal S, et al. (2014) Perinatal exposure to a low dose of bisphenol a ımpaired systemic cellular ımmune response and predisposes young rats to ıntestinal parasitic ınfection. Plos One, 9 (11), 1-15.
• Mikołajewska K, Stragierowicz J, Gromadzińska J (2015). Bisphenol A – Application, sources of exposure, and potential risks in infants, children, and pregnant women. Int J Occup Med Environ Health, 28, 209-241.
• Mulisch M and Welsch U (2015). Romeis mikroskopische technik. (19.ed.) Berlin, springer spektrum. O’Brien E (2013). The effect of bisphenol-a exposure on mast cell function and pulmonary ınflammation associated with asthma (Ph.D. thesis). University of Michigan, Michigan.
• O’Brien E, Dolinoy DC, Mancuso P (2014a). Bisphenol A at concentrations relevant to human exposure enhances histamine and cysteinyl leukotriene release from bone marrow-derived mast cells. J Immunotoxicol, 11 (1), 84–89.
• O’Brien E, Dolinoy DC, Mancuso P (2014b). Perinatal bisphenol A exposures increase the production of pro-inflammatory mediators in bone marrow-derived mast cells of adult mice. J Immunotoxicol, 11 (3), 205–212. Özaydın T, Öznurlu Y, Sur E, Çelik İ, Uluışık D (2018). The effects of bisphenol A on some plasma cytokine levels and distribution of CD8+ and CD4+ T lymphocytes in the spleen, ileal Peyer’s patch, and bronchus-associated lymphoid tissue in rats. Acta Histochemica, 120 (8), 728–733.
• Puxeddu I, Piliponsky AM, Bachelet I, Levi-Schaffer F (2003). Mast cells in allergy and beyond. Int J Biochem Cell Biol, 35 (12), 1601–1607.
• Ramsay DB, Stephen S, Borum M, Voltaggio L, Doman DB (2010). Mast cells in gastrointestinal disease. Gastroenterol Hepatol, 6 (12), 772–777.
• Ribatti D (2018). The staining of mast cells: a historical overview. Int Arch Allergy Immunol, 176 (1), 55–60.
• Sakamoto H, Yokota H, Kibe R, Sayama Y, Yuasa A (2002). Excretion of bisphenol A-glucuronide into the small intestine and deconjugation in the cecum of the rat. Biochim Biophys Acta, 1573 (2), 171–176. Sokol H, Georgin-Lavialle S, Canioni D, et al. (2013). Gastrointestinal manifestations in mastocytosis: A study of 83 patients. J Allergy Clin Immun, 132 (4), 866-873.
• Strobel S, Miller HR, Ferguson A (1981). Human intestinal mucosal mast cells: Evaluation of fixation and staining techniques. J Clin Pathol, 34 (8), 851–858.
• Schwetz BA and Harris MW (1993). Developmental toxicology: status of the field and contribution of the national toxicology program. Environ Health Perspect, 100, 269-282.
• Szymanska K, Makowska K, Gonkowski S (2018). The influence of high and low doses of bisphenol a (BPA) on the enteric nervous system of the porcine ileum. Int J Mol Sci, 19 (3), 917.
• Tikoo S, Barki N, Jain R, et al. (2018). Imaging of mast cells. Int Rev Immunol, 282 (1), 58–72.
• Tolba AM and Mandour DA (2018). Histological effects of bisphenol-A on the reproductive organs of the adult male albino rat. Eur J Anat, 22 (2), 89–102.
• Tung W (1991). Mast cells in the chick digestive tract. II. Fixation, distribution, histochemistry, and ultrastructure. Tokai J Exp Clin Med, 16, 27–32.
• Uslu S and Yörük M (2008). Hindilerde sindirim sisteminde mast hücrelerinin dağılımı ve heterojenitesi üzerine morfolojik ve histometrik araştırmalar. Van Vet J, 19 (2), 47–51.
• Uslu S and Yörük M (2013). Morphological and histometric studies on mast cell distribution and heterogeneity, present in the lower respiratory tract and the lung of local duck (anas platyrhnchase) and goose (anser anser). Kafkas Univ Vet Fak Derg, 19, 475-482.
• Veilleux-Lemieux D, Castel A, Carrier D, Beaudry F, Vachon P (2013). Pharmacokinetics of ketamine and xylazine in young and old sprague–dawley rats. J Am Assoc, 52 (5), 567-570.
• Welle M (1997). Development, significance, and heterogeneity of mast cells with particular regard to the mast cell-specific proteases chymase and tryptase. J Leukoc Biol, 61 (3), 233–245.
• Wernersson S and Pejler G (2014). Mast cell secretory granules: Armed for battle. Nat Rev Immunol, 14 (7), 478–494.