Hyperglycemic Effect of Dietary Boron in Rats with Experimental Diabetes Mellitus Induced by Streptozotocin

Year 2023, Volume: 34 Issue: 3, 244 - 250, 27.11.2023

Nur Akman Alacabey , Hulya Ozdemir , Gökhan Oto

https://doi.org/10.36483/vanvetj.1298344

Cited By: 2

Abstract

In this study, the effect of boric acid (BA) on blood sugar levels, vitamins and minerals in Streptozotocin (STZ)-induced diabetes in rats was investigated. In the study, 48 male Wistar albino rats (200-220 g) were divided into 6 groups, no special treatment was administered to Group1, experimental diabetes was induced by administering STZ (45 mg/kg) through intraperitoneal (IP) injection to other groups. Group 2 diabetes; Group 3 (6 U/kg insulin) insulin; Group 4; 250 ppm, group 5; 500 ppm and group 6 1000 ppm BA mixed with their feed. Blood glucose levels of all groups were quantified from blood taken from the tail vein every week. At the end of study, the rats were sacrificed and their blood was taken. The serum levels of vitamins A, E, and minerals were studied. When compared with other groups, blood glucose levels of groups 4, 5 and 6 were found to be increased (p<0.05). When compared to the baseline values, it was found that Vitamin A in the group 2 and 4, vitamin E in the group 4, Cu in the group 4, 5, 6, Zn, Mg and Na in the group 2 decreased and Fe in the group 2 increased. While Ca and P decreased in groups 5 and 6, no change was observed in Al in all groups. As a result, it has been observed that boron has a hyperglycemic effect when evaluated together with vitamins and minerals in diabetic rats. Whether boron is a suitable agent in the treatment of diabetes should be evaluated with further studies.

Keywords

Diabetes mellitus, Boron, Streptozotocin, Vitamin, Rat, Mineral

Supporting Institution

Van Yüzüncü Yıl University Scientific Research Projects Coordinatorship

Project Number

2010-SBE-YL170

Thanks

We thank Van Yüzüncü Yıl University Scientific Research Projects Coordinatorship for their support to carry out our project.

Streptozotosin ile Deneysel Diyabet Oluşturulan Ratlarda Diyetteki Borun Hiperglisemik Etkisi

Abstract

Bu çalışmada ratlarda Streptozotosin (STZ) ile oluşturulan diyabetes mellitüste borik asidin (BA)'in kan şeker, vitamin ve mineral düzeyleri üzerine etkisi araştırıldı. Sunulan çalışmada 48 adet erkek Wistar albino rat (200-220 g) 6 gruba ayrıldı, Grup 1'e özel bir tedavi uygulanmadı, diğer gruplara intraperitonal (İP) 45 mg/kg STZ uygulanarak deneysel diyabet oluşturuldu. Grup 2 diyabet grubu; Grup 3 (6 U/kg insülin) insülin grubu; Grup 4, 5 ve 6’nın yemlerine sırasıyla 250, 500 ve 1000 ppm BA katıldı. Tüm grupların kan glikoz seviyesi haftalık olarak ölçüldü. Çalışmanın sonunda sıçanlar anestezi altında kan örnekleri alındıktan sonra sakrifiye edildi. Elde edilen serum örneklerinden A ve E vitamin ile bazı mineraller ölçüldü. Kan glikoz düzeylerinin 4, 5 ve 6. gruplarda arttığı belirlendi (p<0,05). Başlangıç değerleri ile karşılaştırıldığında 2 ve 4. grupta A vitamini, 4. grupta E vitamini, 4, 5 ve 6. gruplarda Cu, 2. grupta Zn, Mg, Na ve Fe düzeylerinin azaldığı tespit edildi. 5. ve 6. gruplarda Ca ve P azalırken, tüm gruplarda Al'de değişiklik gözlenmedi. Sonuç olarak, borun diyabetik sıçanlarda vitamin ve mineral düzeyleri ile birlikte değerlendirildiğinde hiperglisemik etkiye sahip olduğu gözlendi. Bor’un diyabet tedavisi için uygun bir ajan olup olmayacağı ileri çalışmalarla değerlendirilmelidir.

Keywords

Bor, Diabetes mellitus, Mineral, Streptozotosin, Sıçan, Vitamin

Project Number

2010-SBE-YL170

References

• Abdelnour SA, Abd El-Hack ME, Swelum AA, Perillo A, Losacco C (2018). The vital roles of boron in animal health and production: A comprehensive review. J Trace Elem Med Biol, 50, 296-304.
• Asmat U, Abad K, Ismail K (2016). Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J, 24 (5), 547-553.
• Bahtiyar N, Hacioglu Y (2019). Tip 2 Diabetes Mellitus Hastalarında Serum Çinko, Selenyum, Mangan, Demir, Bakır Element Düzeyleri ve Cu/Zn, Cu/Se, Cu/Mn, Fe/Zn, Fe/Se, Fe/Mn Oranlarının Değerlendirilmesi. J Sakarya Tıp Dergisi, 9 (1), 38-45.
• Bakken NA, Hunt CD (2003). Dietary boron decreases peak pancreatic in situ insulin release in chicks and plasma insulin concentrations in rats regardless of vitamin D or magnesium status. J Adv Nutr, 133 (11), 3577-3583.
• Bolt HM, Başaran N, Duydu Y (2020). Effects of boron compounds on human reproduction. J Arch Toxicol, 94 (3), 717-724.
• Breydo D (2013). Boron, biologically active compounds. Kretsinger RH, Uversky VN, Permyakov EA. (Ed). Encyclopedia of Metalloproteins (pp. 295-299). Encycl Met Springer, New York.
• Cakir S, Eren M, Senturk M, Sarica ZS (2018). The effect of boron on some biochemical parameters in experimental diabetic rats. J Biol trace elem res, 184 (1),165-172.
• Cebeci E, Yüksel B, Şahin F (2022). Anti-cancer effect of boron derivatives on small-cell lung cancer. J Trace Elem Med Biol, 126923.
• Cho JY, Chang HJ, Lee SK et al. (2007). Amelioration of dextran sulfate sodium-induced colitis in mice by oral administration of β-caryophyllene, a sesquiterpene. Life Sci, 80 (10), 932-939.
• Coban FK, Liman R, Cigerci IH et al. (2015). The antioxidant effect of boron on oxidative stress and DNA damage in diabetic rats. J Fresenius Environ Bull, 24 (11), 4059-4066.
• Darenskaya M, Kolesnikova L, Kolesnikov S (2021). Oxidative stress: Pathogenetic role in diabetes mellitus and its complications and therapeutic approaches to correction. J Bull Exp Biol Med, 171 (2), 179-189.
• Dessordi R, Spirlandeli AL, Zamarioli A, Volpon JB, Navarro AM (2017). Boron supplementation improves bone health of non-obese diabetic mice. J Trace Elem Med Biol, 39, 169-175.
• Domingueti CP, Dusse LMSA, Das Graças Carvalho M et al. (2016). Diabetes mellitus: the linkage between oxidative stress, inflammation, hypercoagulability and vascular complications. J Diabetes Complications, 30 (4),738-745.
• Dubey P, Thakur V, Chattopadhyay M (2020). Role of minerals and trace elements in diabetes and insulin resistance. J Nutrients, 12 (6), 1864.
• Dupree A, Hartmann L, Smith GH, et al. (1994). Spectroscopy of chromospheric lines of giants in the globular cluster. The Astrophysical Journal, 421, 542-549.
• Geyikoğglu F, Türkez H (2007). Acute toxicity of boric acid on energy metabolism of the breast muscle in broiler chickens. J Biologia, 62 (1), 112-117.
• Hakki SS, Dundar N, Kayis SA et al. (2013). Boron enhances strength and alters mineral composition of bone in rabbits fed a high energy diet. J Trace Elem Med Biol, 27 (2), 148-153.
• Hansen AF, Simić A, Åsvold BO, Romundstad P et al. (2017). Trace elements in early phase type 2 diabetes mellitus—A population-based study. The HUNT study in Norway. J Trace Elem Med Biol, 40, 46-53.
• Hunt CD, Herbel JL, Nielsen FH (1997) Metabolic responses of postmenopausal women to supplemental dietary boron and aluminum during usual and low magnesium intake: boron, calcium, and magnesium absorption and retention and blood mineral concentrations. Am J Clin Nutr, 65 (3), 803-813.
• Hunter JM, Nemzer BV, Rangavajla N et al. (2019). The fructoborates: part of a family of naturally occurring sugar–borate complexes—biochemistry, physiology, and impact on human health: a review. J Biol Trace Elem Res, 188 (1), 11-25.
• Karimkhani H, Özkoç M, Shojaolsadati P et al. (2021). Protective effect of boric acid and omega-3 on myocardial infarction in an experimental rat model. J Biol Trace Elem Res, 199 (7), 2612-2620.
• Karatas F, Tug T, Konar V (2008). Serum antioxidant vitamins (A, E, C), selenium and melondialdehyde levels in workers exposed to aerosol. Turkish Thoracic Journal, 9 (1), 13-16.
• Kaur KK, Allahbadia G, Singh M (2021). How Do Minerals along with Trace Elements Influence the Generation of Diabetes Mellitus in Addition to Insulin Resistance: A Systematic Review. J Diab Obes Metab, 4 (1), 125.
• Khalil H (2017). Diabetes microvascular complications—A clinical update. J Diabetes Metab Syndr, 11, 133-139. Khaliq H, Juming Z, Ke-Mei P (2018). The physiological role of boron on health., J Biol Trace Elem Res, 186 (1), 31-51.
• Król E, Jeszka-Skowron M, Krejpcio Z, Flaczyk E, Wójciak R (2016). The Effects of Supplementary Mulberry Leaf (Morus alba) Extracts on the Trace Element Status (Fe, Zn and Cu) in Relation to Diabetes Management and Antioxidant Indices in Diabetic Rats. J Biol Trace Elem Res, 174 (1), 158-165.
• Kucukkurt I, Akbel E, Karabag F, Ince S (2015). The effects of dietary boron compounds in supplemented diet on hormonal activity and some biochemical parameters in rats. J Toxicol Ind Health, 31 (3), 255-260. Martirosyan D, Ashoori MR, Mirmiranpour H (2020). The effect of low level-laser irradiation on antioxidant enzymes and mineral levels in serum of patients with type 2 diabetes mellitus. Bioactive Compounds in Health and Disease, 3 (5), 82-89.
• Muz B, Azab AK, Confalonieri L et al. (2022). Synthesis, equilibrium, and biological study of a C-7 glucose boronic acid derivative as a potential candidate for boron neutron capture therapy. J Bioorg Med Chem, 1 (59), 116659.
• Naghii M, Samman S (1996). The effect of boron supplementation on the distribution of boron in selected tissues and on testosterone synthesis in rats. J. Nutr Biochem, 7 (9), 507-512.
• Narendhirakannan R, Subramanian S, Kandaswamy M (2005). Mineral content of some medicinal plants used in the treatment of diabetes mellitus. J Biol Trace Elem Res, 103 (2), 109-115.
• Nielsen FH. (2014). Update on human health effects of boron. J Trace Elem Med Biol, 28 (4), 383-387.
• Ozel AB, Dagsuyu E, Aydın PK, et al. (2022). Brain Boron Level, DNA Content, and Myeloperoxidase Activity of Metformin-Treated Rats in Diabetes and Prostate Cancer Model. J Trace Elem Med Biol, 200 (3), 1164-1170.
• Özyarım ŞC, Çoban FK (2021). Investigation of The Apoptotic and Antiproliferative Effects of Boron on CCL-233 Human Colon Cancer Cells. Cell J, 23 (4), 429.
• Rad NR, Movahedian A, Feizi A, Aminorroaya A, Aarabi MH (2022). Antioxidant effects of astaxanthin and metformin combined therapy in type 2 diabetes mellitus patients: a randomized double-blind controlled clinical trial. Res Pharm Sci, 17 (2), 219.
• Rahman M, Tushar MAN, Zahid A et al. (2021). Spatiotemporal distribution of boron in the groundwater and human health risk assessment from the coastal region of Bangladesh. Environ Sci Pollut Res Int, 28 (17), 21964-21977.
• Sanjeevi N, Freeland-Graves J, Beretvas SN, Sachdev PK (2018). Trace element status in type 2 diabetes: A meta-analysis. J Clini Diagn Res, 12 (5), OE01-OE08.
• Sevim Ç, Kara, M (2022). Boron and Boron-Containing Compounds Toxicity. Daniel Junqueira Dorta and Danielle Palma de Oliveira (Ed). In The Toxicity of Environmental Pollutants. IntechOpen.
• Sha W, Hu F, Xi Y, Chu Y, Bu S. (2021). Mechanism of ferroptosis and its role in type 2 diabetes mellitus. J Diabetes Res, 2021, 1-10.
• Siddiqui K, Bawazeer N, Scaria Joy S (2014). Variation in macro and trace elements in progression of type 2 diabetes. Scientific World Journal, 2014, 461-591.
• Singh A, Kukreti R, Saso L, Kukreti S (2022). Mechanistic Insight into Oxidative Stress-Triggered Signaling Pathways and Type 2 Diabetes. Molecules, 27 (3), 950.
• Skalnaya MG, Skalny AV, Tinkov AA (2017). Serum copper, zinc, and iron levels, and markers of carbohydrate metabolism in postmenopausal women with prediabetes and type 2 diabetes mellitus J Trace Elem Med Biol, 43, 46-51.
• Sonkar SK, Parmar KS, Ahmad MK, Sonkar GK, Gautam M (2021). An observational study to estimate the level of essential trace elements and its implications in type 2 diabetes mellitus patients. J Family Med Prim Care, 10 (7), 2594-2599.
• Tamrakar S, Kachhawa K (2016). Divya Agraw elements (mg and cu) and dyslipidemia in type 2 diabetes mellitus. Int J Curr Res, 8 (02), 26972-26975.
• Tan SY, Wong JLM, Sim YJ et al. (2019). Type 1 and 2 diabetes mellitus: A review on current treatment approach and gene therapy as potential intervention. Diabetes Metab Syndr, 13 (1), 364-372.
• Vargas MA, Saavedra-Molina A, Gómez-Barroso M et al. (2023). Diazoxide improves muscle function in association with improved dyslipidemia and decreased muscle oxidative stress in streptozotocin-induced diabetic rats. J Bioenerg Biomembr, 55 (1), 71-78.
• Wang C, Kong Z, Duan L et al. (2021). Reproductive toxicity and metabolic perturbations in male rats exposed to boron. Sci Total Environ, 785, 147370.
• Yüztaș, E., Değer, Y., & Yörük, İ. H. (2014). The effects of lycopene on prooxidant/total antioxidant status and vitamin levels in experimental diabetic rats. YYU Vet Fak Derg, 25 (3), 71-75.
• Zatalia SR, Sanusi H (2013). The role of antioxidants in the pathophysiology, complications, and management of diabetes mellitus. Acta Med Indones, 45 (2), 141-147.