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Arı Ekmeğinin (Perga) Kadmiyuma Maruz Kalan Sıçan Böbreğindeki Histopatolojik Değişiklikler ve Apoptoz Belirteçlerinin İmmünohistokimyasal Ekspresyonu Üzerine Etkisi

Year 2024, Volume: 35 Issue: 2, 101 - 108, 31.07.2024
https://doi.org/10.36483/vanvetj.1431490

Abstract

Kadmiyum (Cd), insanlarda ve hayvanlarda çeşitli organlarda toksisiteye neden olan çevresel ve endüstriyel bir kirleticidir. Arı ekmeği (perga), çok çeşitli farmakolojik özelliklere sahip doğal bir flavonoiddir. Bu çalışma, perganın Cd kaynaklı nefrotoksisite üzerindeki etkilerini incelemek amacıyla yapıldı. Otuz iki adet erkek Wistar sıçanı kontrol grubu, Cd grubu (5 mg/kg/gün, oral), Perga grubu (0.5 g/kg/gün, oral) ve Cd+Perga grubu olmak üzere rastgele 4 gruba ayrıldı. 28 günlük deneme süresi sonunda böbrek dokusu örnekleri alınarak histopatolojik, immünohistokimyasal ve biyokimyasal analizler yapıldı. Histopatolojik olarak Cd grubunda, Cd maruziyeti sonucu ciddi tübüler ve glomerüler hasar meydana geldi. İmmünohistokimyasal olarak Cd grubunda böbrek dokusunda caspas-3 ve Bax ekspresyonunda artış vardı. Biyokimyasal sonuçlara göre Cd grubunda katalaz, süperoksit dismutaz ve glutatyon peroksidaz antioksidan enzim seviyeleri azalırken, malondialdehit seviyeleri arttı. Ancak yukarıda belirtilen Cd'nin neden olduğu değişikliklerin çoğu, Perga + Cd grubunda perga tedavisiyle azaldı. Sonuç olarak, perga takviyesi böbrek dokusunda apoptozun inhibisyonu yoluyla Cd kaynaklı böbrek hasarını hafifletebilir.

References

  • Abdel Moneim AE (2016). Indigofera oblongifolia prevents lead acetate-induced hepatotoxicity, oxidative stress, fibrosis and apoptosis in rats. PLoS One, 11, e0158965.
  • Aebi H (1984). Catalase in vitro assay. Meth Enzymol, 105, 121-126.
  • Akkoyun HT (2019). The protective effect of quercetin against to renal tissue damage induced carbontetrachloride in rat. J Inst Sci Technol, 9 (2), 708-716.
  • Akkoyun HT, Uyar A, Bayramoglu Akkoyun M et al. (2023). The protective effect of arbutin against potassium bromate‐induced oxidative damage in the rat brain. J Biochem Mol Toxicol, 37 (2), e23248.
  • Almeer RS, AlBasher GI, Alarifi S et al. (2019). Royal jelly attenuates cadmium-induced nephrotoxicity inmale mice. Sci Rep, 9 (1), 5825.
  • Amanpour P, Khodarahmi P, Salehipour M (2019). Protective effects of vitamin E on cadmium-induced apoptosis in rat testes. Naunyn Schmiedeberg's Arch Pharmacol, 393 (3), 349-358.
  • Bakour M, Al-Waili NS, El Menyiy N et al. (2017). Antioxidant activity and protective effect of bee bread (honey and pollen) in aluminum-induced anemia, elevation of inflammatory makers and hepato-renal toxicity. J Food Sci Technol, 54, 4205-4212.
  • Bakour M, Hammas N, Laaroussi H (2021). Moroccan bee bread improves biochemical and histological changes of the brain, liver, and kidneys induced by titanium dioxide nanoparticles. BioMed Research International. 2021, 1-13.
  • Chora S, Starita-Geribaldi M, Guigonis JM et al. (2009). Effect of cadmium in the clam Ruditapes decussates assessed by proteomic analysis. Aquat Toxicol, 94 (4), 300-308.
  • Çilenk KT, Öztürk İ, Sönmez MF (2016). Ameliorative effect of propolis on the cadmium-induced reproductive toxicity in male albino rats. Exp Mol Pathol, 101 (2), 207-213.
  • EI-Sharaky AS, Newairy AA, Badreldeen MM, Eweda SM, Sheweita SA (2007). Protective role of selenium against renal toxicity induced by cadmium in rats. Toxicology, 235 (3), 185-193.
  • Ercal N, Gurrer-Orhan H, Aykin-Burns N (2001). Toxic metals and oxidative stress. Part I. Mechanisms involved inmetal induced oxidative damage. Curr Top Med Chem, 1 (6), 529-539.
  • Fang J, Xie S, Chen Z et al. (2021). Protective effect of vitamin e on cadmium-induced renal oxidative damage and apoptosis in rats. Biol Trace Elem Res, 199, 4675-4687.
  • Fouad A, Jresat I (2011). Protective effect of telmisartan against cadmium-induced nephrotoxicity in mice. Life Sci, 89, 29-35.
  • Ge J, Zhang C, Sun YC et al. (2019). Cadmium exposure triggers mitochondrial dysfunction and oxidative stress in chicken (Gallus gallus) kidney via mitochondrial UPR inhibition and Nrf2-mediated antioxidant defense activation. Sci Total Environ, 689, 1160-1171.
  • Gong ZG, Wang XY, Wang JH, Fan RF, Wang L (2019). Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem, 192, 62-71.
  • Grosicki A (2004). Influence of vitamin C on cadmium absorption and distribution in rats. J Trace Elem Med Biol, 18 (2), 183-187.
  • Isidorov V, Isidorova A, Sczczepaniak L, Czyzewska U (2009). Gas chromatographic–mass spectrometric investigation of the chemical composition of bee bread. Food Chem, 115 (3), 1056-1063.
  • Jemai H, Messaoudi I, Chaouch A, Kerkeni A (2007). Protective effect of zinc supplementation on blood antioxidant defense system in rats exposed to cadmium. J Trace Elem Med Biol, 21 (4), 269-273.
  • Jomova K, Valko M (2011). Advances in metal-induced oxidative stress and human disease. Toxicology, 283, 65-67.
  • Karabulut-Bulan O, Bolkent S, Yanardag R, Bilgin-Sokmen B (2008). The role of vitamin C, vitamin E, and selenium on cadmium-induced renal toxicity of rats. Drug Chem Toxicol, 31 (4), 413-426.
  • Kieliszek M, Piwowarek K, Kot AM (2018). Pollen and bee bread as new health-oriented products: A review. Trends Food Sci Technol, 71, 170-180.
  • Kosedag M, Gulaboglu M (2023). Pollen and bee bread expressed highest anti-inflammatory activities among bee products in chronic inflammation: an experimental study with cotton pellet granuloma in rats. Inflammopharmacology, 31, 1967-1975.
  • Liu J, Qu W, Kadiiska MB (2009). Role of oxidative stress in cadmium toxicity and carcinogenesis. Toxicol App Pharmacol, 238 (3), 209-214.
  • Lowry O, Rosebrough N, Farr AL, Randall R (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193 (1), 265-275.
  • Messaoudi I, El Heni J, Hammouda F, Saïd K, Kerkeni A (2009). Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biol Trace Elem Res, 130, 152-161.
  • Morales AI, Vicente-Sanchez C, Egido J, Arevalo MA, Lopeznovoa JM (2006). Protective effect of quercetin on experimental chronic cadmium nephrotoxicity in rats is based on its antioxidant properties. Food Chem Toxicol, 44, 2092-2100.
  • Nagai T, Nagashima T, Suzuki N, Inoue R (2005). Antioxidant activity and angiotensin I–converting enzyme inhibition by enzymatic hydrolysates from bee bread. Zeitschrift für Naturforschung C, 60 (1-2), 133-138.
  • Nordberg GF (2009). Historical perspectives on cadmium toxicology. Toxicol Appl Pharmacol, 238 (3), 192-200. Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358.
  • Omar EM, El-Sayed NS, Elnozahy FY et al. (2023). Reversal effects of royal jelly and propolis against cadmium-induced hepatorenal toxicity in rats. Biol Trace Elem Res, 1-16.
  • Paglia DE, Valentine WN (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
  • Pari L, Murugavel P, Sitasawad SL, Kumar KS (2007). Cytoprotective and antioxidant role of diallyl tetrasulfide on cadmium induced renal injury. An in vivo and in vitro study. Life Sci, 80 (7), 650-658.
  • Pathak, N, Khandelwal S (2006). Oxidative stress and apoptotic changes in murine splenocytes exposed to cadmium. Toxicology, 220 (1), 26-36.
  • Renugadevi J, Prabu SM (2009). Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology, 256 (1-2), 128-134.
  • Renugadevi J, Prabu SM (2010). Quercetin protects against oxidative stress-related renal dysfunction by cadmium in rats. Exp Toxicol Path, 62 (5), 471-481.
  • Sanchez-Chardi A, Penarroja-Matutano C, Borras M, Nadal J (2009). Bioaccumulation of metals and effects of a landfill in small mammals Part III: structural alterations. Environ Res, 109 (8), 960-967.
  • Shao CC, Li N, Zhang ZW et al. (2014). Cadmium supplement triggers endoplasmic reticulum stress response and cytotoxicity in primary chicken hepatocytes. Ecotoxicol Environ Saf, 106, 109-114.
  • Soltaninejad K, Kebriaeezadeh A, Minaiee B et al. (2003). Biochemical and ultrastructural evidences for toxicity of lead through free radicals in rat brain. Hum Exp Toxicol, 22 (8), 417-423.
  • Suhartono E, Triawanti T, Leksono AS (2015). Effects of cadmium exposure on lipid peroxidation and chlorinative stress of rat kidney. J Exp Life Sci, 5 (1), 1-5.
  • Sun Y, Oberley LW, Li Y (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem, 34 (3), 497-500.
  • Szymonic-Lesiuk S, Chechowska G, Stryjecka M et al. (2003). Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat after carbon tetrachloride intoxication. J Hepatobiliary Pancreat Surg, 10, 309-315.
  • Tripathi S, Srivastav AK (2011). Cytoarchitectural alterations in kidney of Wistar rat after oral exposure to cadmium chloride. Tissue and Cell, 43 (2), 131-136.
  • Vásquez A, Olofsson TC (2009). The lactic acid bacteria involved in the production of bee pollen and bee bread. J Apic Res, 48 (3), 189-195.
  • Waisberg M, Joseph P, Hale B, Beyersmann D (2003). Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology, 192 (2-3), 95-117.
  • Wang Y, Wu Y, Luo K (2013). The protective effects of selenium on cadmium-induced oxidative stress and apoptosis via mitochondria pathway in mice kidney. Food Chem Toxicol, 58 (6), 61-67.
  • Watjen W, Beyermann D (2004). Cadmium induced apoptosis in C6 gliomacells. Influence of oxidative stress: Biometals, 17, 65-78.
  • Wilmes A, Crean D, Aydin S (2011). Identification and dissection of the Nrf2 mediated oxidative stress pathway in human renal proximal tubule toxicity. Toxicol In Vitro, 25 (3), 613-622.
  • Wolff NA, Lee WK, Abouhamed M, Thevenod F (2008). Role of ARF6 in internalization of metal-binding proteins, metallothionein and transferring, and cadmium-metallothionein toxicity in kidney proximal tubule cells. Toxicol Appl Pharmacol, 230 (1), 78-85.
  • Yaman T, Aydemir C (2021). Respiratory syncytial virus infection induces expression of inducible nitric oxide synthase, CD3, and CD8 in naturally occurring pneumonia in lambs. Acta Vet, 71 (2), 170-188.
  • Yaman T, Kömüroğlu AU (2018). Effects of juniperus communis L. oil on nephropathy in experimental diabetic rats. Harran Üniv Vet Fak Derg, 7 (2), 192-199.
  • Yuan G, Dai S, Yin Z (2014). Sub-chronic lead and cadmium co-induce apoptosis protein expression in liver and kidney of rats. Int J Clin Exp Pathol, 7 (6), 2905.
  • Zakaria Z, Othman ZA, Suleiman JB (2021). Hepatoprotective effect of bee bread in metabolic dysfunction-associated fatty liver disease (MAFLD) rats: Impact on oxidative stress and inflammation. Antioxidants, 10 (12), 2031.
  • Zerdani Z, Abouda I, Kalalou I, Faid M, Ahami MT (2011). The antibacterial activity of Moroccan bee bread and bee-pollen (fresh and dried) against pathogenic bacteria. Res J Microbiol, 6, 376-384.
  • Zhuang J, Nie G, Yang F (2019). Cadmium induces cytotoxicity through oxidative stressmediated apoptosis pathway in duck renal tubular epithelial cells. Toxicol in Vitro, 61, 1-10.

Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium

Year 2024, Volume: 35 Issue: 2, 101 - 108, 31.07.2024
https://doi.org/10.36483/vanvetj.1431490

Abstract

Cadmium (Cd) is an environmental and industrial pollutant that causes toxicity in various organs in humans and animals. Bee bread (perga) is a natural flavonoid with a wide range of pharmacological properties. This study was conducted to examine the effects of perga on Cd-induced nephrotoxicity. Thirty-two male Wistar rats were randomly divided into 4 groups, as the Control group, Cd group (5 mg/kg/day, orally), Perga group (0.5 g/kg/day, orally), and Cd + Perga group. At the end of the 28-day experiment, kidney tissue samples were taken and histopathological, immunohistochemical, and biochemical analyses were performed. Histopathologically, severe tubular and glomerular damage occurred as a result of Cd exposure in the Cd group. Immunohistochemically, there was an increase in caspas-3 and Bax expression in the renal tissue in the Cd group. According to the biochemical results, while the catalase, superoxide dismutase, and glutathione peroxidase antioxidant enzyme levels decreased in the Cd group, and the malondialdehyde levels increased. However, most of the above-mentioned Cd-induced changes were attenuated by treatment with perga in the Perga + Cd group. In conclusion, perga supplementation may alleviate Cd-induced renal injury through inhibition of apoptosis in renal tissue.

Ethical Statement

The local ethics committee of Van Yüzüncü Yıl University Animal Experiments approved the study (01/06/2023, 2023/07-12).

References

  • Abdel Moneim AE (2016). Indigofera oblongifolia prevents lead acetate-induced hepatotoxicity, oxidative stress, fibrosis and apoptosis in rats. PLoS One, 11, e0158965.
  • Aebi H (1984). Catalase in vitro assay. Meth Enzymol, 105, 121-126.
  • Akkoyun HT (2019). The protective effect of quercetin against to renal tissue damage induced carbontetrachloride in rat. J Inst Sci Technol, 9 (2), 708-716.
  • Akkoyun HT, Uyar A, Bayramoglu Akkoyun M et al. (2023). The protective effect of arbutin against potassium bromate‐induced oxidative damage in the rat brain. J Biochem Mol Toxicol, 37 (2), e23248.
  • Almeer RS, AlBasher GI, Alarifi S et al. (2019). Royal jelly attenuates cadmium-induced nephrotoxicity inmale mice. Sci Rep, 9 (1), 5825.
  • Amanpour P, Khodarahmi P, Salehipour M (2019). Protective effects of vitamin E on cadmium-induced apoptosis in rat testes. Naunyn Schmiedeberg's Arch Pharmacol, 393 (3), 349-358.
  • Bakour M, Al-Waili NS, El Menyiy N et al. (2017). Antioxidant activity and protective effect of bee bread (honey and pollen) in aluminum-induced anemia, elevation of inflammatory makers and hepato-renal toxicity. J Food Sci Technol, 54, 4205-4212.
  • Bakour M, Hammas N, Laaroussi H (2021). Moroccan bee bread improves biochemical and histological changes of the brain, liver, and kidneys induced by titanium dioxide nanoparticles. BioMed Research International. 2021, 1-13.
  • Chora S, Starita-Geribaldi M, Guigonis JM et al. (2009). Effect of cadmium in the clam Ruditapes decussates assessed by proteomic analysis. Aquat Toxicol, 94 (4), 300-308.
  • Çilenk KT, Öztürk İ, Sönmez MF (2016). Ameliorative effect of propolis on the cadmium-induced reproductive toxicity in male albino rats. Exp Mol Pathol, 101 (2), 207-213.
  • EI-Sharaky AS, Newairy AA, Badreldeen MM, Eweda SM, Sheweita SA (2007). Protective role of selenium against renal toxicity induced by cadmium in rats. Toxicology, 235 (3), 185-193.
  • Ercal N, Gurrer-Orhan H, Aykin-Burns N (2001). Toxic metals and oxidative stress. Part I. Mechanisms involved inmetal induced oxidative damage. Curr Top Med Chem, 1 (6), 529-539.
  • Fang J, Xie S, Chen Z et al. (2021). Protective effect of vitamin e on cadmium-induced renal oxidative damage and apoptosis in rats. Biol Trace Elem Res, 199, 4675-4687.
  • Fouad A, Jresat I (2011). Protective effect of telmisartan against cadmium-induced nephrotoxicity in mice. Life Sci, 89, 29-35.
  • Ge J, Zhang C, Sun YC et al. (2019). Cadmium exposure triggers mitochondrial dysfunction and oxidative stress in chicken (Gallus gallus) kidney via mitochondrial UPR inhibition and Nrf2-mediated antioxidant defense activation. Sci Total Environ, 689, 1160-1171.
  • Gong ZG, Wang XY, Wang JH, Fan RF, Wang L (2019). Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem, 192, 62-71.
  • Grosicki A (2004). Influence of vitamin C on cadmium absorption and distribution in rats. J Trace Elem Med Biol, 18 (2), 183-187.
  • Isidorov V, Isidorova A, Sczczepaniak L, Czyzewska U (2009). Gas chromatographic–mass spectrometric investigation of the chemical composition of bee bread. Food Chem, 115 (3), 1056-1063.
  • Jemai H, Messaoudi I, Chaouch A, Kerkeni A (2007). Protective effect of zinc supplementation on blood antioxidant defense system in rats exposed to cadmium. J Trace Elem Med Biol, 21 (4), 269-273.
  • Jomova K, Valko M (2011). Advances in metal-induced oxidative stress and human disease. Toxicology, 283, 65-67.
  • Karabulut-Bulan O, Bolkent S, Yanardag R, Bilgin-Sokmen B (2008). The role of vitamin C, vitamin E, and selenium on cadmium-induced renal toxicity of rats. Drug Chem Toxicol, 31 (4), 413-426.
  • Kieliszek M, Piwowarek K, Kot AM (2018). Pollen and bee bread as new health-oriented products: A review. Trends Food Sci Technol, 71, 170-180.
  • Kosedag M, Gulaboglu M (2023). Pollen and bee bread expressed highest anti-inflammatory activities among bee products in chronic inflammation: an experimental study with cotton pellet granuloma in rats. Inflammopharmacology, 31, 1967-1975.
  • Liu J, Qu W, Kadiiska MB (2009). Role of oxidative stress in cadmium toxicity and carcinogenesis. Toxicol App Pharmacol, 238 (3), 209-214.
  • Lowry O, Rosebrough N, Farr AL, Randall R (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193 (1), 265-275.
  • Messaoudi I, El Heni J, Hammouda F, Saïd K, Kerkeni A (2009). Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biol Trace Elem Res, 130, 152-161.
  • Morales AI, Vicente-Sanchez C, Egido J, Arevalo MA, Lopeznovoa JM (2006). Protective effect of quercetin on experimental chronic cadmium nephrotoxicity in rats is based on its antioxidant properties. Food Chem Toxicol, 44, 2092-2100.
  • Nagai T, Nagashima T, Suzuki N, Inoue R (2005). Antioxidant activity and angiotensin I–converting enzyme inhibition by enzymatic hydrolysates from bee bread. Zeitschrift für Naturforschung C, 60 (1-2), 133-138.
  • Nordberg GF (2009). Historical perspectives on cadmium toxicology. Toxicol Appl Pharmacol, 238 (3), 192-200. Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358.
  • Omar EM, El-Sayed NS, Elnozahy FY et al. (2023). Reversal effects of royal jelly and propolis against cadmium-induced hepatorenal toxicity in rats. Biol Trace Elem Res, 1-16.
  • Paglia DE, Valentine WN (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
  • Pari L, Murugavel P, Sitasawad SL, Kumar KS (2007). Cytoprotective and antioxidant role of diallyl tetrasulfide on cadmium induced renal injury. An in vivo and in vitro study. Life Sci, 80 (7), 650-658.
  • Pathak, N, Khandelwal S (2006). Oxidative stress and apoptotic changes in murine splenocytes exposed to cadmium. Toxicology, 220 (1), 26-36.
  • Renugadevi J, Prabu SM (2009). Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology, 256 (1-2), 128-134.
  • Renugadevi J, Prabu SM (2010). Quercetin protects against oxidative stress-related renal dysfunction by cadmium in rats. Exp Toxicol Path, 62 (5), 471-481.
  • Sanchez-Chardi A, Penarroja-Matutano C, Borras M, Nadal J (2009). Bioaccumulation of metals and effects of a landfill in small mammals Part III: structural alterations. Environ Res, 109 (8), 960-967.
  • Shao CC, Li N, Zhang ZW et al. (2014). Cadmium supplement triggers endoplasmic reticulum stress response and cytotoxicity in primary chicken hepatocytes. Ecotoxicol Environ Saf, 106, 109-114.
  • Soltaninejad K, Kebriaeezadeh A, Minaiee B et al. (2003). Biochemical and ultrastructural evidences for toxicity of lead through free radicals in rat brain. Hum Exp Toxicol, 22 (8), 417-423.
  • Suhartono E, Triawanti T, Leksono AS (2015). Effects of cadmium exposure on lipid peroxidation and chlorinative stress of rat kidney. J Exp Life Sci, 5 (1), 1-5.
  • Sun Y, Oberley LW, Li Y (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem, 34 (3), 497-500.
  • Szymonic-Lesiuk S, Chechowska G, Stryjecka M et al. (2003). Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat after carbon tetrachloride intoxication. J Hepatobiliary Pancreat Surg, 10, 309-315.
  • Tripathi S, Srivastav AK (2011). Cytoarchitectural alterations in kidney of Wistar rat after oral exposure to cadmium chloride. Tissue and Cell, 43 (2), 131-136.
  • Vásquez A, Olofsson TC (2009). The lactic acid bacteria involved in the production of bee pollen and bee bread. J Apic Res, 48 (3), 189-195.
  • Waisberg M, Joseph P, Hale B, Beyersmann D (2003). Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology, 192 (2-3), 95-117.
  • Wang Y, Wu Y, Luo K (2013). The protective effects of selenium on cadmium-induced oxidative stress and apoptosis via mitochondria pathway in mice kidney. Food Chem Toxicol, 58 (6), 61-67.
  • Watjen W, Beyermann D (2004). Cadmium induced apoptosis in C6 gliomacells. Influence of oxidative stress: Biometals, 17, 65-78.
  • Wilmes A, Crean D, Aydin S (2011). Identification and dissection of the Nrf2 mediated oxidative stress pathway in human renal proximal tubule toxicity. Toxicol In Vitro, 25 (3), 613-622.
  • Wolff NA, Lee WK, Abouhamed M, Thevenod F (2008). Role of ARF6 in internalization of metal-binding proteins, metallothionein and transferring, and cadmium-metallothionein toxicity in kidney proximal tubule cells. Toxicol Appl Pharmacol, 230 (1), 78-85.
  • Yaman T, Aydemir C (2021). Respiratory syncytial virus infection induces expression of inducible nitric oxide synthase, CD3, and CD8 in naturally occurring pneumonia in lambs. Acta Vet, 71 (2), 170-188.
  • Yaman T, Kömüroğlu AU (2018). Effects of juniperus communis L. oil on nephropathy in experimental diabetic rats. Harran Üniv Vet Fak Derg, 7 (2), 192-199.
  • Yuan G, Dai S, Yin Z (2014). Sub-chronic lead and cadmium co-induce apoptosis protein expression in liver and kidney of rats. Int J Clin Exp Pathol, 7 (6), 2905.
  • Zakaria Z, Othman ZA, Suleiman JB (2021). Hepatoprotective effect of bee bread in metabolic dysfunction-associated fatty liver disease (MAFLD) rats: Impact on oxidative stress and inflammation. Antioxidants, 10 (12), 2031.
  • Zerdani Z, Abouda I, Kalalou I, Faid M, Ahami MT (2011). The antibacterial activity of Moroccan bee bread and bee-pollen (fresh and dried) against pathogenic bacteria. Res J Microbiol, 6, 376-384.
  • Zhuang J, Nie G, Yang F (2019). Cadmium induces cytotoxicity through oxidative stressmediated apoptosis pathway in duck renal tubular epithelial cells. Toxicol in Vitro, 61, 1-10.
There are 54 citations in total.

Details

Primary Language English
Subjects Veterinary Pathology
Journal Section Araştırma Makaleleri
Authors

Turan Yaman 0000-0001-8811-9775

Turan Akkoyun 0000-0001-6391-3905

Ömer Faruk Keleş 0000-0002-7869-5311

Mahire Bayramoğlu Akkoyun 0000-0001-5150-5402

Early Pub Date July 25, 2024
Publication Date July 31, 2024
Submission Date February 5, 2024
Acceptance Date April 3, 2024
Published in Issue Year 2024 Volume: 35 Issue: 2

Cite

APA Yaman, T., Akkoyun, T., Keleş, Ö. F., Bayramoğlu Akkoyun, M. (2024). Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium. Van Veterinary Journal, 35(2), 101-108. https://doi.org/10.36483/vanvetj.1431490
AMA Yaman T, Akkoyun T, Keleş ÖF, Bayramoğlu Akkoyun M. Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium. Van Vet J. July 2024;35(2):101-108. doi:10.36483/vanvetj.1431490
Chicago Yaman, Turan, Turan Akkoyun, Ömer Faruk Keleş, and Mahire Bayramoğlu Akkoyun. “Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium”. Van Veterinary Journal 35, no. 2 (July 2024): 101-8. https://doi.org/10.36483/vanvetj.1431490.
EndNote Yaman T, Akkoyun T, Keleş ÖF, Bayramoğlu Akkoyun M (July 1, 2024) Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium. Van Veterinary Journal 35 2 101–108.
IEEE T. Yaman, T. Akkoyun, Ö. F. Keleş, and M. Bayramoğlu Akkoyun, “Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium”, Van Vet J, vol. 35, no. 2, pp. 101–108, 2024, doi: 10.36483/vanvetj.1431490.
ISNAD Yaman, Turan et al. “Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium”. Van Veterinary Journal 35/2 (July 2024), 101-108. https://doi.org/10.36483/vanvetj.1431490.
JAMA Yaman T, Akkoyun T, Keleş ÖF, Bayramoğlu Akkoyun M. Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium. Van Vet J. 2024;35:101–108.
MLA Yaman, Turan et al. “Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium”. Van Veterinary Journal, vol. 35, no. 2, 2024, pp. 101-8, doi:10.36483/vanvetj.1431490.
Vancouver Yaman T, Akkoyun T, Keleş ÖF, Bayramoğlu Akkoyun M. Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium. Van Vet J. 2024;35(2):101-8.

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