Determination of Haptoglobin, Serum Amyloid A, Some Other Acute Phase Proteins, and Biochemical Parameters in Cattle with Hydatid Cysts

Year 2024, Volume: 35 Issue: 3, 160 - 163, 28.11.2024

Kadir Bozukluhan , Oğuz Merhan , Enes Akyüz , Semistan Kızıltepe , Uğur Aydın , Tahir Gezer , Derya Kocamaz , Gürbüz Gökçe

https://doi.org/10.36483/vanvetj.1488935

Abstract

The aim of this study is to determine some acute phase proteins and biochemical parameter levels in cattle infected with the hydatid cysts. In the study, a total of 30, Brown Swiss cattle including 15 infected with the hydatid cysts in the study group and 15 in the control group were used. Haptoglobin, serum amyloid A (SAA), ceruloplasmin, interleukin (IL)-6, total protein, albumin, aspartate amino transferase (AST), gamma glutamyl transferase (GGT) and alkaline phosphatase (ALP) activity, urea, creatinine, iron (Fe) levels were determined colorimetrically. When the animals infected with hydatid cysts and control group were compared, it was determined that haptoglobin, SAA, IL-6, AST, ALP, GGT activity, urea, creatinine levels and acute phase protein index values increased, while albumin levels decreased. In addition, it was determined that the ceruloplasmin and total protein levels increased, globulin, albumin/globulin and Fe levels decreased, but they were statistically insignificant. In conclusion, it was determined that there were significant changes in the biochemical parameters and increased haptoglobin and SAA synthesis in the animals infected with the hydatid cysts, and it is thought that these parameters may contribute to the pathogenesis and diagnosis of the disease.

Keywords

Cattle, Haptoglobin, Hydatid cysts, Serum amyloid A

Hidatik Kistli Sığırlarda Haptoglobin, Serum Amiloid A ve Diğer Bazı Akut Faz Proteinleri ile Biyokimyasal Parametrelerin Belirlenmesi

Abstract

Çalışmada amaç hidatik kist ile enfekte sığırlarda bazı akut faz proteinleri ve biyokimyasal parametre düzeylerinin belirlenmesidir. Çalışmada çalışma grubunda hidatik kist ile enfekte 15, kontrol grubunda 15 olmak üzere toplam 30 adet Montofon sığır kullanıldı. Haptoglobin, serum amiloid A (SAA), seruloplazmin, interlökin (IL)-6, total protein, albümin, aspartat amino transferaz (AST), gama glutamil transferaz (GGT) ve alkalin fosfataz (ALP) aktivitesi, üre, kreatinin, demir (Fe) düzeyleri kolorimetrik olarak belirlendi. Hidatik kist ile enfekte hayvanlar ile kontrol grubu karşılaştırıldığında, haptoglobin, SAA, IL-6, AST, ALP, GGT aktivitesi, üre, kreatinin düzeyleri ve akut faz protein indeksi değerlerinin arttığı, albümin düzeylerinin ise azaldığı belirlendi. Ayrıca seruloplazmin ve total protein düzeylerinin arttığı, globulin, albümin/globulin ve Fe düzeylerinin ise azaldığı ancak istatistiksel olarak anlamlı olmadığı belirlendi. Sonuç olarak hidatik kist ile enfekte hayvanlarda biyokimyasal parametrelerde önemli değişiklikler, haptoglobin ve SAA sentezinde artış olduğu belirlenmiş olup bu parametrelerin hastalığın patogenezine ve tanısına katkı sağlayabileceği düşünülmektedir.

Keywords

Haptoglobin, Hidatik kistler, Sığır, Serum amiloid A

References

• Abdulkhaleq LA, Assi MA, Abdullah R et al. (2018). The crucial roles of inflammatory mediators in inflammation: A review. Vet World, 11, 627-635.
• Acıöz M (2018). Intensive hydatid cyst case in cattle. Mid Blac Sea J Health Sci, 4, 31-33.
• Aral H (2015). Laboratory in diagnosis and monitoring of systemic inflammatory response syndrome or sepsis. Eur J Basic Med Sc, 5, 10-20.
• Avcıoğlu H (2013). Sığırlarda Karaciğer ve Diğer İç Organlarda Görülen Helmint Hastalıkları. 1. Baskı. Meta Press, İzmir.
• Ayaz E, Ertekin A, Özdal N, Taş Z (2006). Some biochemical parameters in sheep infected with endoparasites (Fasciola spp., Dicrocoelium dendriticum, hydatid cysts, Trichostrongylidae and Protostrongylidae). Turkiye Parazitol Derg, 30, 57-61.
• Balkaya I, Şimşek S (2010). Erzurum’da kesilen sığırlarda hidatidosis ve fasciolosis’in yaygınlığı ve ekonomik önemi. Kafkas Univ Vet Fak Derg, 16, 793-797.
• Bayraktar M, Mehmet N, Durmaz R (2005). Th1 and Th2 inducing cytokines in cystic echinococcosis. Turkiye Parazitol Derg, 29, 167-170.
• Bayyit E, Merhan O (2020). Normal ve güç doğum yapan ineklerde bazı akut faz proteinlerinin ve oksidatif stres düzeyinin belirlenmesi. Atatürk Üniversitesi Vet Bil Derg, 15, 145-150.
• Bozukluhan K, Merhan O, Özcan A, Gökçe Hİ, Gökçe G (2017). Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi. Ankara Univ Vet Fak Derg, 64, 75-79.
• Bozukluhan K, Merhan O, Ogun M et al. (2018-a). Investigation of haptoglobin, serum amyloid A, and some biochemical parameters in calves with omphalitis. Vet World, 11, 1055-1058.
• Bozukluhan K, Merhan O, Gokce HI et al. (2018-b). Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected sheeppox virus. Kafkas Univ Vet Fak Derg, 24, 437-441.
• Bozukluhan K, Merhan O, Kiziltepe S et al. (2021). Determination of haptoglobin, some biochemical and oxidative stress parameters in calves with pneumonia. Fresenius Environ Bull, 30, 9485-9489.
• Colombo JP, Richterich R (1964). Zur bestimmung des caeruloplasmin im plasma [on the determination of ceruloplasmin in plasma]. Schweiz Med Wochenschr, 23, 715-720.
• Comba B, Mert H, Comba A, Mis L, Mert N (2017). Some hematological and biochemical parameters in Karakul and Norduz sheep. Van Vet J, 28, 137-140.
• Cınar M, Aydenizoz M, Gokpınar S, Camkerten G (2018). Evaluation of biochemical parameters and oxidative stress in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts. Turk J Vet Anim Sci, 42, 423-428.
• Eckersall PD, Bell R (2010). Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27.
• Enemark HL, Bille-Hansen V, Lind P et al. (2003). Pathogenicity of Cryptosporidium parvum-evaluation of an animal infection model. Vet Parasitol, 113, 35-57.
• Ganheim C, Höglund J, Waller KP (2004). Acute phase proteins in response to Dictyocaulus viviparus infection in calves. Acta Vet Scand, 45, 79-86.
• Gokce HI, Woldehiwet Z (1999). The effects of Ehrlichia (Cytoecetes) phagocytophila on the clinical chemistry of sheep and goats. Zentralbl Veterinarmed B, 46, 93-103.
• Gökçe Hİ, Bozukluhan K, Uzlu E (2009). Kronik solunum yetmezliği bulunan bir sığırda hydatid kist olgusu. Dicle Üniv Vet Fak Derg, 1, 15-18.
• Haniloo A, Ghasemi F, Shikhi AK, Ghavami MB (2008). Immunoregulatory cytokine (TGF-β and IL-10) responses in mice inoculated with protoscoleces and major hydatid fluid antigens of cystic echinococcosis. Iranian J Parasitol, 3, 18-23.
• Hoffmann WE, Solter PF (2008). Diagnostic enzymology of domestic animals. 1. Edition. Academic Press, New York.
• Iliev PT, Geoergiva TM (2018). Acute phase proteins in sheep and goats-functıon, refrence ranges and assessment methods: an overview. Bulg J Vet Med, 21, 1-16.
• Irak K, Çelik OY, Aslan Çelik B et al. (2019). Investigation of some biochemical parameters in sheep naturally infected with cystic echinococcosis. KSU J Agric Nat, 22, 948-952.
• Kırbaş A, Kaman R, Merhan O et al. (2021). Evaluation of acute phase response and oxidative damage in sheep naturally infected with streptococcus pluranimalium using haptoglobin, nitric oxide, and malondialdehyde levels. FU Vet J Health Sci, 35, 145-150.
• Martinez-Subiela S, Ceron JJ (2005). Evaluation of acute phase protein indexes in dogs with leishmaniasis at diagnosis, during and after short-term treatment. Vet Med-Czech, 50, 39-46.
• Merhan O, Bozukluhan K, Gokce HI (2017). Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. J Hell Vet Medical Soc, 68 (4), 535-540.
• Merhan O, Erkılıç EE, Bozukluhan K, Öğün M, Kırmızıgül AH (2021). Bulaşıcı ektimalı koyunlarda haptoglobin, serum amiloid A ve seruloplazmin düzeylerinin araştırılması. FÜ Sağ Bil Vet Derg, 35, 77-80.
• Mohammadi S, Mohammadi V, Esmaeilnejad B (2021). Evaluation of some acute phase proteins in cattle naturally infected with Babesia bigemina. Comp Immunol Microbiol Infect Dis, 76, 101642.
• Moro P, Schantz PM (2009). Echinococcosis: a review. Int J Infect Dis, 13, 125-133.
• Omidi A, Sabzevari S, Nazifi S (2017). Acute-phase proteins and antioxidant status in cows naturally infected by hydatid cysts in the Razavi Khorasan Province. IJRHR, 2, 1-7.
• Pradeep M (2014). Application of acute phase proteins as biomarkers in modern veterinary practice. Ind J Vet and Anim Sci Res, 43, 1-13.
• Regassa B (2019). Review on hydatidosis in small ruminant and its economic and public health significance. JDVS, 11, 1-8.
• Rigano R, Buttari B, De Falco E et al. (2004). Echinococcus granulosus-specific T-cell lines derived from patients at various clinical stages of cystic echinococcosis. Parasite Immunol, 26, 45-52.
• Sevimli A, Sevimli FK, Seker E, Ulucan A, Demirel HH (2015). Acute-phase responses in cattle infected with hydatid cysts and microbial agents. J Helminthol, 89, 471-479.
• Skinner JG, Brown RA, Roberts L (1991). Bovine haptoglobin response in clinically defined field conditions. Vet Rec, 128, 147-149.
• Şahin T, Akgül Y (2006). Investigation of some trace element levels and biochemical parameters in sheep with endoparasite. Van Health Sci J, 9, 100-106.
• Tennant BC, Center SA (2008). Hepatic function. 6. Edition. Academic Press, New York.
• Tothova C, Nagy O, Kovac G (2014). Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med (Praha), 59, 163-180.
• Ulutaş PA, Voyvoda H, Ulutaş B, Aypak S (2008). Miks helmint enfeksiyonlu keçilerde haptoglobin, serum amiloid-A ve seruloplazmin konsantrasyonları. Türkiye Parazitol Derg, 32, 229-233.