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Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells

Year 2023, , 59 - 66, 15.01.2023
https://doi.org/10.33188/vetheder.1183380

Abstract

The objective of this study is to determine the effects of bee venom on the proliferation capacity of mesenchymal stem cells and wound healing. For this purpose, mesenchymal stem cells were isolated from canine adipose tissue and bee venom samples were collected from Apis mellifera anatoliaca in Muğla province of Türkiye. Cell viability test was performed on mesenchymal stem cells exposed to various concentrations (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm and 0.312 ppm) of bee venom. And wound healing test was performed on cells treated with the doses (5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm) and imaged every two hours for 16 hours. According to the results of our study's cell proliferation assay and wound healing test, bee venom had no proliferative effect on mesenchymal stem cells within the defined dose range. The study's outcomes may be enhanced by investigating the effect of bee venom on mesenchymal stem cells in combination with other substances or by improving the bee venom's purification process. Even while we have a better understanding of the mechanisms of action of bee venom components, there are still a lot of unanswered questions on the subject. It is believed that figuring out how bee venom affects wound healing may be useful for advancing wound care in both veterinary and human medicine.

References

  • 1. Somwongin S, Chantawannakul P, Chaiyana W. Antioxidant activity and irritation property of venoms from Apis species. Toxicon 2018;145:32-39.
  • 2. Bellik Y. Bee venom: its potential use in alternative medicine. Anti-infective agents 2015;13: 3-16.
  • 3. Moreno M, Giralt E. Three valuable peptides from bee and wasp venoms for therapeutic and biotechnological use: melittin, apamin and mastoparan. Toxins 2015;7:1126-1150.
  • 4. Wehbe R, Frangieh J, Rima M, El Obeid D, Sabatier JM, Fajloun Z. Bee venom: Overview of main compounds and bioactivities for therapeutic interests. Molecules 2019;24(16):2997-2298.
  • 5. Hozzein WN, Badr G, Al Ghamdi AA, Sayed A, Al-Waili NS, Garraud O. Topical application of propolis enhances cutaneous wound healing by promoting TGF-beta/Smad-mediated collagen production in a streptozotocin-induced type I diabetic mouse model. Cellular Physiology and Biochemistry 2015;37(3):940-954.
  • 6. Goharshenasan P, Amini S, Atria A, Abtahi H, Khorasani G. Topical application of honey on surgical wounds: A randomized clinical trial. Complementary Medicine Research 2016;23(1):12-15.
  • 7. Friedenstein AJ, Piatetzky SII, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol. 1966;16: 381-390.
  • 8. Huang YZ, Gou M, Da LC, Zhang WQ, Xie HQ. Mesenchymal stem cells for chronic wound healing: current status of preclinical and clinical studies. Tissue Engineering Part B: Reviews 2020;26(6):555-570.
  • 9. Frangieh J, Salma Y, Haddad K, Mattei C, Legros C, Fajloun Z, El Obeid D. First Characterization of The Venom from Apis mellifera syriaca, A Honeybee from The Middle East Region. Toxins 2019;11:191-192.
  • 10. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose Tissue: Implications for cell-based therapies. Tissue Engineering 2001;7: 211-228.

Arı zehirinin yağ dokusu kaynaklı mezenkimal kök hücre üzerindeki yara iyileştirici etkileri

Year 2023, , 59 - 66, 15.01.2023
https://doi.org/10.33188/vetheder.1183380

Abstract

Bu çalışmanın amacı, arı zehirinin mezenkimal kök hücrelerin çoğalma kapasitesi ve yara iyileşmesi üzerindeki etkilerini belirlemektir. Bu amaçla köpek yağ dokusundan mezenkimal kök hücreler izole edilmiş ve Muğla çevresinde bulunan Apis mellifera anatoliaca'dan arı zehiri örnekleri toplanmıştır. Farklı konsantrasyonlarda arı zehirine maruz bırakılan mezenkimal kök hücreler üzerinde hücre canlılığı testi (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0,625 ppm ve 0.312 ppm) yapılmıştır. Hücreler üzerinde 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm arı zehri kullanılarak yara iyileştirme deneyi yapılmış ve 16 saat boyunca her iki saatte bir görüntülenmiştir. Çalışmanın hücre canlılığı ve yara iyileşme deneylerinin sonuçlarına göre, arı zehirinin köpek yağ dokusu kaynaklı mezenkimal kök hücreler üzerinde tanımlanan doz aralığında proliferatif bir etkisi olmamıştır. Çalışmanın sonuçları, diğer maddelerle birlikte mezenkimal kök hücreler üzerindeki arı zehirinin etkisini araştırarak veya arı zehirinin saflaştırma sürecini geliştirerek iyileştirilebilir. Arı zehiri bileşenlerinin etki mekanizmalarını daha iyi anlamış olsak da konuyla ilgili hala cevaplanmamış birçok soru bulunmaktadır. Arı zehrinin yara iyileşmesi üzerindeki etkisinin belirlenmesinin hem veteriner hekimlikte hem de beşeri hekimlikte yara tedavisini kolaylaştırmada avantaj sağlayacağı düşünülmektedir.

References

  • 1. Somwongin S, Chantawannakul P, Chaiyana W. Antioxidant activity and irritation property of venoms from Apis species. Toxicon 2018;145:32-39.
  • 2. Bellik Y. Bee venom: its potential use in alternative medicine. Anti-infective agents 2015;13: 3-16.
  • 3. Moreno M, Giralt E. Three valuable peptides from bee and wasp venoms for therapeutic and biotechnological use: melittin, apamin and mastoparan. Toxins 2015;7:1126-1150.
  • 4. Wehbe R, Frangieh J, Rima M, El Obeid D, Sabatier JM, Fajloun Z. Bee venom: Overview of main compounds and bioactivities for therapeutic interests. Molecules 2019;24(16):2997-2298.
  • 5. Hozzein WN, Badr G, Al Ghamdi AA, Sayed A, Al-Waili NS, Garraud O. Topical application of propolis enhances cutaneous wound healing by promoting TGF-beta/Smad-mediated collagen production in a streptozotocin-induced type I diabetic mouse model. Cellular Physiology and Biochemistry 2015;37(3):940-954.
  • 6. Goharshenasan P, Amini S, Atria A, Abtahi H, Khorasani G. Topical application of honey on surgical wounds: A randomized clinical trial. Complementary Medicine Research 2016;23(1):12-15.
  • 7. Friedenstein AJ, Piatetzky SII, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol. 1966;16: 381-390.
  • 8. Huang YZ, Gou M, Da LC, Zhang WQ, Xie HQ. Mesenchymal stem cells for chronic wound healing: current status of preclinical and clinical studies. Tissue Engineering Part B: Reviews 2020;26(6):555-570.
  • 9. Frangieh J, Salma Y, Haddad K, Mattei C, Legros C, Fajloun Z, El Obeid D. First Characterization of The Venom from Apis mellifera syriaca, A Honeybee from The Middle East Region. Toxins 2019;11:191-192.
  • 10. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose Tissue: Implications for cell-based therapies. Tissue Engineering 2001;7: 211-228.
There are 10 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section RESEARCH ARTICLE
Authors

Özge Özgenç 0000-0002-8776-4788

Sedat Sevin 0000-0003-0475-9092

Publication Date January 15, 2023
Submission Date October 13, 2022
Acceptance Date December 21, 2022
Published in Issue Year 2023

Cite

Vancouver Özgenç Ö, Sevin S. Wound healing effects of bee venom on adipose tissue derived mesenchymal stem cells. Vet Hekim Der Derg. 2023;94(1):59-66.

Veteriner Hekimler Derneği Dergisi açık erişimli bir dergi olup, derginin yayın modeli Budapeşte Erişim Girişimi (BOAI) bildirisine dayanmaktadır. Yayınlanan tüm içerik, çevrimiçi ve ücretsiz olarak sunulan Creative Commons CC BY-NC 4.0 lisansı altında lisanslanmıştır. Yazarlar, Veteriner Hekimler Derneği Dergisi'nde yayınlanan eserlerinin telif haklarını saklı tutarlar.


Veteriner Hekimler Derneği / Turkish Veterinary Medical Society