Short communication expression of glial fibrillary acidic protein in some tumors of dogs

Year 2011, Volume: 82 Issue: 1 - Sayı: 1 Cilt: 82, 23 - 27, 01.01.2011

Fehmi Arıkan Hamdi Uysal

Abstract

Glial Fibriler Asidik Protein (GFAP), molekül ağırlığı 50 kDa olan merkezi sinir sistemine ait astrositlerin intermediyer filament bir proteinidir. Sinir dokusunun tanımlanmasında bir antijen olarak kullanılan GFAP, merkezi sinir sisteminde

Keywords

Glial fibriler asidik protein, tümör, köpek, tümör belirteçleri

Bazı Köpek Tümörlerinde Glial Fibriler Asidik Protein Ekspresyonu

Abstract

Glial Fibrillary Acidic Protein (GFAP) is an intermediate filament protein in astrocytes of the Central Nervous System that has a molecular weight of approximately 50 kDa. GFAP, as an antigen in nervous tissue identification, is used as a reliable immunohistochemical marker for glial neoplastic cells and benign astrocytoma cells. In veterinary medicine breast cancers, skin neoplasms and lymphatic tumors are frequent. In scientific literatures it has been made clear that GFAP is a remarkable marker for the normal and neoplastic glial cells nevertheless there is no information about GFAP as a marker for the tumors not generally considered to be of glial origin. Therefore, in this study to determine GFAP in the several dog tumors except glial neoplasms was intended. The results of Sodium Dodecyl Sulfate Polyacrylamide Gel Electroforesis (SDS-PAGE) and Semi-Dry Blotting analysis using antibody for GFAP (immunoblotting) showed that GFAP is not being expressed by the 14 tumors which we studied. It has been observed that GFAP expression is in enough amount in the brain tissues. The fact that GFAP has not been identified in the tumor tissues we analyzed points out that this protein is specific to nervous system tissues

Keywords

Glial fibrillary acidic protein, tumor, dogs, tumor markers