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Çiğ süt ve peynirlerde koagulaz pozitif stafilokoklar

Year 2016, Volume: 87 Issue: 2, 3 - 12, 01.04.2016

Abstract

Stafilokokal intoksikasyonlar (SE), pek çok ülkede en sık görülen bakteriyel gıda kaynaklı zehirlenmeler arasında ilk sıralarda yer almaktadır. SE’ler başta Staphylococcus aureus olmak üzere bazı koagulaz pozitif stafilokok (KPS) ve diğer stafilokoklar tarafından da oluşturulmaktadır. Bu çalışma, Amasya bölgesinde tüketime sunulan 50 çiğ süt ve 60 peynir örneğinde KPS ile kontaminasyon düzeylerini araştırmak amacıyla yapıldı. Bu kapsamda, mikrokok/stafilokok ve takiben KPS klasik kültür tekniği ile izole edilerek fenotipik yöntemlerle identifiye edildi. Analiz bulguları çerçevesinde; 60 peynir örneğin 40’ında toplam mikrokok/stafilokok düzeyi 102 - 107 kob/g (koloni oluşturan birim) arasında belirlendi. KPS 60 örneğin 18’inden 102 ila 106kob/g düzeylerinde saptandı. Kontaminasyon düzeylerinin dağılımı; 2 (%3,33), 5 (%8,33) 3 (%5,00) ve 7 (%11,66) örnekte sırasıyla 102, 103, 104 ve 105 kob/g, 1(%1,66) örnekte ise 106 kob/g idi. Çiğ süt analiz bulguları çerçevesinde, mikrokok/stafilokok 50 örneğin 49’unda 102 - 106 kob/ml düzeylerinde izole edildi. KPS ise 50 örneğin 32 (%64)’sinden izole edildi. Kontaminasyon düzeylerinin dağılımı; 11 (%22,00), 19 (%38,00) ve 2 (%4,00) örnekte sırasıyla 102, 103 ve104 kob/ml idi. Sonuç olarak, 11 (%18,33) peynir ve 2 (%4) çiğ süt örneğinin KPS yönünden

Coagulase positive staphylococci in raw milk and cheese

Year 2016, Volume: 87 Issue: 2, 3 - 12, 01.04.2016

Abstract

Stapylococcal intoxication is the most frequently occurred food-poising among the food-borne poising cases in many countries. Generally the enterotoxin production is associated with Staphylococcus aureus apart from other Staphylococcus species. Therefore, the aim of the study was to investigate the present of coagulase positive Staphylococci (CPS) in 50 raw milk and 60 varieties of cheese samples consumed in Amasya province, Turkey. For this, micrococci/staphylococci following CPS were isolated using classic culture technique and identified using phenotypic methods. According to the results, total number of micrococci/staphylococci were found to be between 102 and 107 cfu/ g (colony forming unit) in 40 out of 60 cheese samples. Among these, CPS was isolated 18 of 60 cheese samples at levels between 102 and 106 cfu/g. According to distribution of contamination levels; 2 (3.33%), 5 (8.33%) 3 (5.00%) and 7 (11.66%) of the samples contained 102, 10, 104 and 105 cfu/g CPS. Only 1(1.66%) of the sample contained 106 cfu/g CPS. It was also analyzed 50 raw milk samples. In conclusion, micrococci/staphylococci were isolated 49 out of 50 samples at the levels of 102 - 106 cfu/ml. CPS was also detected in 32 (64.00%) of 50 samples. According to distribution levels, it was detected at the levels of 102, 103 and 104 cfu/ml in 11 (22.00%) 19 (38.00%) and two (4.00%) of the samples. As a result, 11 (18,33%) of cheese and 2 (4.00%) raw milk samples with regard to CPS were not accaptable according to Turk Food Codex

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Details

Other ID JA36SY57YB
Journal Section Research Article
Authors

Tuba Yıldırım This is me

Belgin Sırıken This is me

Ceren Yavuz This is me

Publication Date April 1, 2016
Submission Date April 1, 2016
Published in Issue Year 2016 Volume: 87 Issue: 2

Cite

Vancouver Yıldırım T, Sırıken B, Yavuz C. Coagulase positive staphylococci in raw milk and cheese. Vet Hekim Der Derg. 2016;87(2):3-12.

Veteriner Hekimler Derneği Dergisi (Journal of Turkish Veterinary Medical Society) is an open access publication, and the journal’s publication model is based on Budapest Access Initiative (BOAI) declaration. All published content is licensed under a Creative Commons CC BY-NC 4.0 license, available online and free of charge. Authors retain the copyright of their published work in Veteriner Hekimler Derneği Dergisi (Journal of Turkish Veterinary Medical Society). 

Veteriner Hekimler Derneği / Turkish Veterinary Medical Society