Evaluation of Pregnancy-Associated Glycoprotein (PAG) Levels for the Detection of Pseudopregnancy in Aleppo Goats

Year 2025, Volume: 4 Issue: 1, 25 - 33, 30.06.2025

Ömer Yaprakcı , Tuğra Akkuş

https://doi.org/10.61262/vetjku.1665802

Abstract

Aim to study: This study aimed to determine the PAG concentration based on pregnancy age in Aleppo goats and evaluate its usability in diagnosing pseudopregnancy.
Material ve methods: The animal material of the study consisted of 70 Aleppo goats aged 2-5 years, each having given birth at least once. The goats were divided into seven groups according to the anamnesis provided by the owners and the B-mode ultrasonography examinations. Group 1 (n=10): One-month pregnant goats, Group 2 (n=10): Two-month pregnant goats, Group 3 (n=10): Three-month pregnant goats, Group 4 (n=10): Four-month pregnant goats, Group 5 (n=10): Five-month pregnant goats, Group 6 (n=10): Non-pregnant goats, and Group 7 (n=10): Pseudopregnant goats. Progesterone and PAG levels in the study groups were determined using commercial kits. The obtained data were analyzed using one-way ANOVA.
Results: Significant differences were observed in the PAG and progesterone levels between the groups (P<0.001). The group with the highest progesterone level was Group 3, while the lowest progesterone level was found in Group 6 (P<0.001). However, no statistically significant difference in progesterone levels was found between Groups 2, 3, and 4 (P>0.05). Regarding PAG levels, the highest value was found in Group 3, and the lowest in Group 6 (P<0.001). Additionally, no significant difference in PAG levels was found between Groups 6 and 7 (P>0.05).
Conclusion: It was determined that PAG levels could be used as a reliable biomarker for diagnosing pseudopregnancy during all stages of pregnancy in Aleppo goats.

Keywords

Aleppo Goat , PAG , progesterone , pseudopregnancy.

Ethical Statement

This study was conducted with the approval of the Harran University Local Ethics Committee for Animal Experiments (HRÜ-HADYEK) under permit number 2025/003/11.

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