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Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats

Year 2023, Volume: 18 Issue: 3, 112 - 116, 20.12.2023

Abstract

Heat stress (HS) has negative effects on reproductive parameters in male animals. This study was designed to investigate the protective effects of Vitis vinifera L., which has a natural antioxidant property, on reproductive parameters, testicular oxidative stress, reproductive hormones, and testicular histopathology in male rats treated with HS. Fifty male rats were divided into 5 different groups (control, HS, HS + 100, HS + 200, and HS + 300). It was determined that subsieve V. vinifera extract given at different doses eliminated the negative effects of HS administration on reproductive parameters. It was determined that V. vinifera increased sperm motility and density and decreased the rate of abnormal sperm regardless of dose (P < .001). It was determined that increased oxidative stress and decreased luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels as a result of HS administration improved in the extract-treated groups (P < .05), and FSH and testosterone levels increased in the HS + 200 and HS + 300 groups compared to the control group (P < .001). Histopathologically, it was determined that the degeneration, edema, and congestion caused by HS disappeared due to the extract administration. As a result of this study, the protective effect of subsieve V. vinifera extract on HS on male reproductive parameters was revealed.

References

  • 1. Shahat AM, Rizzoto G, Kastelic JP. Amelioration of heat stressinduced damage to testes and sperm quality. Theriogenology. 2020;158:84-96. [CrossRef] 2. Gasco L, Biancarosa I, Liland NS. From waste to feed: a review of recent knowledge on insects as producers of protein and fat for animal feeds. Curr Opin Green Sustain Chem. 2020;23:67-79. [CrossRef]
  • 3. Kim B, Park K, Rhee K. Heat stress response of male germ cells. Cell Mol Life Sci. 2013;70(15):2623-2636. [CrossRef]
  • 4. Aldahhan RA, Stanton PG. Heat stress response of somatic cells in the testis. Mol Cell Endocrinol. 2021;527:111216. [CrossRef]
  • 5. Morrell JM. Heat stress and bull fertility. Theriogenology. 2020;153:62- 67. [CrossRef]
  • 6. Rizzoto G, Kastelic JP. A new paradigm regarding testicular thermoregulation in ruminants? Theriogenology. 2020;147:166-175. [CrossRef]
  • 7. Akdenİz B, Demİrbüker Kavak D. Physicochemical characteristics and antioxidant capacity of traditional yogurt fortified with grape (Vitis vinifera L.) seed extract at different levels. Kocatepe Veterinary Journal. 2019:1-1. [CrossRef]
  • 8. Di Stefano V, Buzzanca C, Melilli MG, et al. Polyphenol characterization and antioxidant activity of grape seeds and skins from Sicily: a preliminary study. Sustainability. 2022;14(11):6702. [CrossRef]
  • 9. Athanasiadis V, Palaiogiannis D, Poulianiti K, Bozinou E, Lalas SI, Makris DP. Extraction of polyphenolic antioxidants from red grape pomace and olive leaves: process optimization using a tailor-made tertiary deep eutectic solvent. Sustainability. 2022;14(11):6864. [CrossRef]
  • 10. Okumuş E. Effect of different drying processes on antioxidant and antidiabetic properties of pomegranate press wastes. Yüzüncü Yıl Univ Fen Bilimleri Enstitüsü Derg. 2023;28(1):113-120. [CrossRef]
  • 11. Test No. 436. Acute Oral Toxicity. Acute Toxic Class Method; OECD Guideline for Testing of Chemicals, Section 4. Paris: The Organisation for Economic Co-operation and Development (OECD); 2009. [CrossRef]
  • 12. Hafez ESE, Hafez B. Semen evaluation. In: Reproduction in Farm Animals. 7th ed. Wiley-Blackwell; 2013:365-375.
  • 13. Witko-Sarsat V, Friedlander M, Capeillère-Blandin C, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996;49(5):1304-1313. [CrossRef]
  • 14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-358. [CrossRef]
  • 15. Lartillot S, Kedziora P, Athias A. Purification and characterization of a new fungal catalase. Prep Biochem. 1988;18(3):241-246. [CrossRef]
  • 16. Matsushita K, Miyake H, Chiba K, Fujisawa M. Clusterin produced by Sertoli cells inhibits heat stress-induced apoptosis in the rat testis. Andrologia. 2016;48(1):11-19. [CrossRef]
  • 17. Qin DZ, Cai H, He C, et al. Melatonin relieves heat-induced spermatocyte apoptosis in mouse testes by inhibition of ATF6 and PERK signaling pathways. Zool Res. 2021;42(4):514-524. [CrossRef]
  • 18. Sui J, Feng Y, Li H, Cao R, Tian W, Jiang Z. Baicalin protects mouse testis from injury induced by heat stress. J Therm Biol. 2019;82:63- 69. [CrossRef]
  • 19. Durairajanayagam D, Agarwal A, Ong C. Causes, effects and molecular mechanisms of testicular heat stress. Reprod Biomed Online. 2015;30(1):14-27. [CrossRef]
  • 20. Ikeda M, Kodama H, Fukuda J, et al. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biol Reprod. 1999;61(2):393-399. [CrossRef]
  • 21. Matsuki S, Iuchi Y, Ikeda Y, Sasagawa I, Tomita Y, Fujii J. Suppression of cytochrome c release and apoptosis in testes with heat stress by minocycline. Biochem Biophys Res Commun. 2003;312(3):843-849. [CrossRef]
  • 22. Paul C, Teng S, Saunders PTK, Single A, Mild. Transient scrotal heat stress causes hypoxia and oxidative stress in mouse testes, which induces germ cell Death1. Biol Reprod. 2009;80(5):913-919. [CrossRef]
  • 23. Sailer BL, Sarkar LJ, Bjordahl JA, Jost LK, Evenson DP. Effects of heat stress on mouse testicular cells and sperm chromatin structure. J Androl. 1997;18(3):294-301. [CrossRef]
  • 24. Alawi NA, Hag GAE, Rabah SO, et al. The protective effect of grape (Vitis vinifera) seed oil on testicular structure of male rats exposed to lead. ABR. 2018;12(1):16-25.
  • 25. Oladokun OO, Osuntokun OS, Adedokun KI, et al. The hypothalamic-pituitaryte sticu lar axis disruption following haloperidol treatment in male Wistar rats: protective role of proanthocyanidins rich fraction of Vitis vinifera seed. FUOYE. 2022;2:11-25. [CrossRef]
  • 26. Hafsa SHA, Ayman HA, Elham AS, Popescu A, Mocuta D. Grape Seed (Vitis vinifera l.) Alleviate Reproductive Toxicity Caused by Lindane in New Zeeland White Male Rabbits. 2016;16(3):17-28. 27. Atere TG, Adewumi OE, Fatoki JO, et al. Proanthocyanidin from Vitis vinifera modulates testicular dysfunction associated with manganese toxicity in Wistar rats via suppression of DNA degradation, inflammation and oxidative stress. FUOYE. 2022;2:26-37. [CrossRef]
  • 28. Khattab AH, Abdallah ZA, Kamel M. Grape seed extract alleviate reproductive toxicity caused by aluminium chloride in male rats. Am J Sci. 2010;6(12):1200-1209.
  • 29. Liu Z, Stocco DM. Heat shock-induced inhibition of acute steroidogenesis in MA-10 cells is associated with inhibition of the synthesis of the steroidogenic acute regulatory protein. Endocrinology. 1997;138(7):2722-2728. [CrossRef]
  • 30. Oka S, Shiraishi K, Fujimoto M, et al. Role of heat shock Factor 1 in conserving cholesterol transportation in Leydig cell steroidogenesis via steroidogenic acute regulatory protein. Endocrinology. 2017;158(8):2648-2658. [CrossRef]
  • 31. Halder S, Sarkar M, Dey S, Kumar Bhunia S, Ranjan Koley A, Giri B. Protective effects of red grape (Vitis vinifera) juice through restoration of antioxidant defense, endocrine swing and Hsf1, Hsp72 levels in heat stress induced testicular dysregulation of Wister rat. J Therm Biol. 2018;71:32-40. [CrossRef]

Elekaltı Üzüm Ekstraktının (Vitis Vinifera L.) Sıcaklık Stresi Uygulanan Ratlarda Testis, Reprodüktif Hormonlar ve Bazı Spermatolojik Parametreler Üzerine Koruyucu Etkisi

Year 2023, Volume: 18 Issue: 3, 112 - 116, 20.12.2023

Abstract

Sıcaklık stresi (HS), erkek hayvanlarda üreme parametreleri üzerinde olumsuz etkilere sahiptir. Bu çalışma, doğal antioksidan özelliği olan Vitis vinifera L.’nin HS’ye maruz bırakılan erkek sıçanlarda üreme parametreleri, testiküler oksidatif stres, üreme hormonları ve testis histopatolojisi üzerindeki koruyucu etkilerini araştırmak amacıyla planlandı. 50 erkek rat 5 farklı gruba ayrıldı (Kontrol, HS, HS + 100, HS + 200, HS + 300). Farklı dozlarda verilen elekaltı V. vinifera ekstraktının üreme parametreleri üzerindeki olumsuz etkilerini ortadan kaldırdığı tespit edildi. V. vinifera’nin dozdan bağımsı olarak sperm motilite, yoğunluğunu arttırdığı ve anormal sperm oranını azalttığı belirlendi(P < ,001). HS uygulaması sonucu artan oksidatif stres ve azalan lüteinleştirici hormon (LH), folikül uyarıcı hormon (FSH) ve Testosteron düzeylerinin ekstrakt uygulanan gruplarda iyileştiği (P < ,05), ayrıca HS + 200 ve HS + 300 gruplarında FSH ve Testosteron düzeylerinin kontrol grubuna oranala arttığı belirlendi (P < ,001). Histopatolojik olarak HS’nin neden olduğu dejenerasyon, ödem ve konjesyonların ekstrakt uygulamasına bağlı olarak ortadan kalktığı belirlendi. Bu çalışma sonucunda elekaltı V. vinifera HS uygulamasına bağlı olarak bozulan erkek üreme parametreleri üzerindeki koruyucu etkisi ortaya konmuştur.

References

  • 1. Shahat AM, Rizzoto G, Kastelic JP. Amelioration of heat stressinduced damage to testes and sperm quality. Theriogenology. 2020;158:84-96. [CrossRef] 2. Gasco L, Biancarosa I, Liland NS. From waste to feed: a review of recent knowledge on insects as producers of protein and fat for animal feeds. Curr Opin Green Sustain Chem. 2020;23:67-79. [CrossRef]
  • 3. Kim B, Park K, Rhee K. Heat stress response of male germ cells. Cell Mol Life Sci. 2013;70(15):2623-2636. [CrossRef]
  • 4. Aldahhan RA, Stanton PG. Heat stress response of somatic cells in the testis. Mol Cell Endocrinol. 2021;527:111216. [CrossRef]
  • 5. Morrell JM. Heat stress and bull fertility. Theriogenology. 2020;153:62- 67. [CrossRef]
  • 6. Rizzoto G, Kastelic JP. A new paradigm regarding testicular thermoregulation in ruminants? Theriogenology. 2020;147:166-175. [CrossRef]
  • 7. Akdenİz B, Demİrbüker Kavak D. Physicochemical characteristics and antioxidant capacity of traditional yogurt fortified with grape (Vitis vinifera L.) seed extract at different levels. Kocatepe Veterinary Journal. 2019:1-1. [CrossRef]
  • 8. Di Stefano V, Buzzanca C, Melilli MG, et al. Polyphenol characterization and antioxidant activity of grape seeds and skins from Sicily: a preliminary study. Sustainability. 2022;14(11):6702. [CrossRef]
  • 9. Athanasiadis V, Palaiogiannis D, Poulianiti K, Bozinou E, Lalas SI, Makris DP. Extraction of polyphenolic antioxidants from red grape pomace and olive leaves: process optimization using a tailor-made tertiary deep eutectic solvent. Sustainability. 2022;14(11):6864. [CrossRef]
  • 10. Okumuş E. Effect of different drying processes on antioxidant and antidiabetic properties of pomegranate press wastes. Yüzüncü Yıl Univ Fen Bilimleri Enstitüsü Derg. 2023;28(1):113-120. [CrossRef]
  • 11. Test No. 436. Acute Oral Toxicity. Acute Toxic Class Method; OECD Guideline for Testing of Chemicals, Section 4. Paris: The Organisation for Economic Co-operation and Development (OECD); 2009. [CrossRef]
  • 12. Hafez ESE, Hafez B. Semen evaluation. In: Reproduction in Farm Animals. 7th ed. Wiley-Blackwell; 2013:365-375.
  • 13. Witko-Sarsat V, Friedlander M, Capeillère-Blandin C, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996;49(5):1304-1313. [CrossRef]
  • 14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-358. [CrossRef]
  • 15. Lartillot S, Kedziora P, Athias A. Purification and characterization of a new fungal catalase. Prep Biochem. 1988;18(3):241-246. [CrossRef]
  • 16. Matsushita K, Miyake H, Chiba K, Fujisawa M. Clusterin produced by Sertoli cells inhibits heat stress-induced apoptosis in the rat testis. Andrologia. 2016;48(1):11-19. [CrossRef]
  • 17. Qin DZ, Cai H, He C, et al. Melatonin relieves heat-induced spermatocyte apoptosis in mouse testes by inhibition of ATF6 and PERK signaling pathways. Zool Res. 2021;42(4):514-524. [CrossRef]
  • 18. Sui J, Feng Y, Li H, Cao R, Tian W, Jiang Z. Baicalin protects mouse testis from injury induced by heat stress. J Therm Biol. 2019;82:63- 69. [CrossRef]
  • 19. Durairajanayagam D, Agarwal A, Ong C. Causes, effects and molecular mechanisms of testicular heat stress. Reprod Biomed Online. 2015;30(1):14-27. [CrossRef]
  • 20. Ikeda M, Kodama H, Fukuda J, et al. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biol Reprod. 1999;61(2):393-399. [CrossRef]
  • 21. Matsuki S, Iuchi Y, Ikeda Y, Sasagawa I, Tomita Y, Fujii J. Suppression of cytochrome c release and apoptosis in testes with heat stress by minocycline. Biochem Biophys Res Commun. 2003;312(3):843-849. [CrossRef]
  • 22. Paul C, Teng S, Saunders PTK, Single A, Mild. Transient scrotal heat stress causes hypoxia and oxidative stress in mouse testes, which induces germ cell Death1. Biol Reprod. 2009;80(5):913-919. [CrossRef]
  • 23. Sailer BL, Sarkar LJ, Bjordahl JA, Jost LK, Evenson DP. Effects of heat stress on mouse testicular cells and sperm chromatin structure. J Androl. 1997;18(3):294-301. [CrossRef]
  • 24. Alawi NA, Hag GAE, Rabah SO, et al. The protective effect of grape (Vitis vinifera) seed oil on testicular structure of male rats exposed to lead. ABR. 2018;12(1):16-25.
  • 25. Oladokun OO, Osuntokun OS, Adedokun KI, et al. The hypothalamic-pituitaryte sticu lar axis disruption following haloperidol treatment in male Wistar rats: protective role of proanthocyanidins rich fraction of Vitis vinifera seed. FUOYE. 2022;2:11-25. [CrossRef]
  • 26. Hafsa SHA, Ayman HA, Elham AS, Popescu A, Mocuta D. Grape Seed (Vitis vinifera l.) Alleviate Reproductive Toxicity Caused by Lindane in New Zeeland White Male Rabbits. 2016;16(3):17-28. 27. Atere TG, Adewumi OE, Fatoki JO, et al. Proanthocyanidin from Vitis vinifera modulates testicular dysfunction associated with manganese toxicity in Wistar rats via suppression of DNA degradation, inflammation and oxidative stress. FUOYE. 2022;2:26-37. [CrossRef]
  • 28. Khattab AH, Abdallah ZA, Kamel M. Grape seed extract alleviate reproductive toxicity caused by aluminium chloride in male rats. Am J Sci. 2010;6(12):1200-1209.
  • 29. Liu Z, Stocco DM. Heat shock-induced inhibition of acute steroidogenesis in MA-10 cells is associated with inhibition of the synthesis of the steroidogenic acute regulatory protein. Endocrinology. 1997;138(7):2722-2728. [CrossRef]
  • 30. Oka S, Shiraishi K, Fujimoto M, et al. Role of heat shock Factor 1 in conserving cholesterol transportation in Leydig cell steroidogenesis via steroidogenic acute regulatory protein. Endocrinology. 2017;158(8):2648-2658. [CrossRef]
  • 31. Halder S, Sarkar M, Dey S, Kumar Bhunia S, Ranjan Koley A, Giri B. Protective effects of red grape (Vitis vinifera) juice through restoration of antioxidant defense, endocrine swing and Hsf1, Hsp72 levels in heat stress induced testicular dysregulation of Wister rat. J Therm Biol. 2018;71:32-40. [CrossRef]

Details

Primary Language English
Subjects Veterinary Sciences (Other)
Journal Section Research Articles
Authors

Volkan KOŞAL

Publication Date December 20, 2023
Published in Issue Year 2023 Volume: 18 Issue: 3

Cite

APA KOŞAL, V. (2023). Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats. Veterinary Sciences and Practices, 18(3), 112-116.
AMA KOŞAL V. Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats. Veterinary Sciences and Practices. December 2023;18(3):112-116.
Chicago KOŞAL, Volkan. “Protective Effect of Subsieve Grape Extract (Vitis Vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats”. Veterinary Sciences and Practices 18, no. 3 (December 2023): 112-16.
EndNote KOŞAL V (December 1, 2023) Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats. Veterinary Sciences and Practices 18 3 112–116.
IEEE V. KOŞAL, “Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats”, Veterinary Sciences and Practices, vol. 18, no. 3, pp. 112–116, 2023.
ISNAD KOŞAL, Volkan. “Protective Effect of Subsieve Grape Extract (Vitis Vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats”. Veterinary Sciences and Practices 18/3 (December 2023), 112-116.
JAMA KOŞAL V. Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats. Veterinary Sciences and Practices. 2023;18:112–116.
MLA KOŞAL, Volkan. “Protective Effect of Subsieve Grape Extract (Vitis Vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats”. Veterinary Sciences and Practices, vol. 18, no. 3, 2023, pp. 112-6.
Vancouver KOŞAL V. Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats. Veterinary Sciences and Practices. 2023;18(3):112-6.

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