Protective Effect of Subsieve Grape Extract (Vitis vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats

Yıl 2023, Cilt: 18 Sayı: 3, 112 - 116, 20.12.2023

Volkan Koşal

Öz

Heat stress (HS) has negative effects on reproductive parameters in male animals. This study was designed to investigate the protective effects of Vitis vinifera L., which has a natural antioxidant property, on reproductive parameters, testicular oxidative stress, reproductive hormones, and testicular histopathology in male rats treated with HS. Fifty male rats were divided into 5 different groups (control, HS, HS + 100, HS + 200, and HS + 300). It was determined that subsieve V. vinifera extract given at different doses eliminated the negative effects of HS administration on reproductive parameters. It was determined that V. vinifera increased sperm motility and density and decreased the rate of abnormal sperm regardless of dose (P < .001). It was determined that increased oxidative stress and decreased luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels as a result of HS administration improved in the extract-treated groups (P < .05), and FSH and testosterone levels increased in the HS + 200 and HS + 300 groups compared to the control group (P < .001). Histopathologically, it was determined that the degeneration, edema, and congestion caused by HS disappeared due to the extract administration. As a result of this study, the protective effect of subsieve V. vinifera extract on HS on male reproductive parameters was revealed.

Anahtar Kelimeler

Heat stress, sperm, testis, Vitis vinifera L.

Elekaltı Üzüm Ekstraktının (Vitis Vinifera L.) Sıcaklık Stresi Uygulanan Ratlarda Testis, Reprodüktif Hormonlar ve Bazı Spermatolojik Parametreler Üzerine Koruyucu Etkisi

Öz

Sıcaklık stresi (HS), erkek hayvanlarda üreme parametreleri üzerinde olumsuz etkilere sahiptir. Bu çalışma, doğal antioksidan özelliği olan Vitis vinifera L.’nin HS’ye maruz bırakılan erkek sıçanlarda üreme parametreleri, testiküler oksidatif stres, üreme hormonları ve testis histopatolojisi üzerindeki koruyucu etkilerini araştırmak amacıyla planlandı. 50 erkek rat 5 farklı gruba ayrıldı (Kontrol, HS, HS + 100, HS + 200, HS + 300). Farklı dozlarda verilen elekaltı V. vinifera ekstraktının üreme parametreleri üzerindeki olumsuz etkilerini ortadan kaldırdığı tespit edildi. V. vinifera’nin dozdan bağımsı olarak sperm motilite, yoğunluğunu arttırdığı ve anormal sperm oranını azalttığı belirlendi(P < ,001). HS uygulaması sonucu artan oksidatif stres ve azalan lüteinleştirici hormon (LH), folikül uyarıcı hormon (FSH) ve Testosteron düzeylerinin ekstrakt uygulanan gruplarda iyileştiği (P < ,05), ayrıca HS + 200 ve HS + 300 gruplarında FSH ve Testosteron düzeylerinin kontrol grubuna oranala arttığı belirlendi (P < ,001). Histopatolojik olarak HS’nin neden olduğu dejenerasyon, ödem ve konjesyonların ekstrakt uygulamasına bağlı olarak ortadan kalktığı belirlendi. Bu çalışma sonucunda elekaltı V. vinifera HS uygulamasına bağlı olarak bozulan erkek üreme parametreleri üzerindeki koruyucu etkisi ortaya konmuştur.

Anahtar Kelimeler

Sıcaklık stresi, sperm, testis, Vitis vinifera L

Kaynakça

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