Ratların Gebelik Döneminde Tükettikleri Krill Yağlı Yoğurt İçeren Diyetin Yavrularının Uzun Kemikleri Üzerindeki Etkisi

Year 2024, Volume: 19 Issue: 3, 155 - 163, 29.12.2024

İftar Gürbüz , Zeki Erol , Yasin Demiraslan , Ayşe Nur Özen Duran , Halil Yalçın

https://doi.org/10.17094/vetsci.1458449

Abstract

Gebelikte yavruların kemik gelişimini desteklemek için kalsiyum, vitamin, mineral ve omega 3 yağ asitlerinin kullanımı önerilmektedir. Bu çalışmanın amacı, bu özelliklerden zengin olan krill yağı ile karıştırılmış probiyotik yoğurt ile gebe ratları beslemek ve yavrularında uzun kemiklerin morfometrik gelişimini incelemektir. Bu amaçla deney grubunda 6 adet (krill yağı ile karıştırılmış yoğurtla beslenen gebe ratların yavruları) ve kontrol grubunda 6 adet (standart yemle beslenen gebe ratların yavruları) olmak üzere toplam 12 adet 2 aylık yavru rat çalışmada kullanıldı. Yavru ratlar 2 aylık olduklarında biyometrik ölçümleri alındı. Ötanazi sonrasında yavruların uzun kemikleri (Humerus, antebrachium, femur, ossa cruris) maserasyonla temizlendi. Bu kemikler fotoğraflandı. Bu kemiklerin uzunluk ve genişliklerinin morfometrik ölçümleri Image J programı kullanılarak yapıldı. SPSS (20.0 Versiyonu) programında sağ ve sol bacak kemikleri arasında ve kontrol ve deney grupları arasında Independent Samples T testi ile parametreler karşılaştırıldı. Ayrıca parametreler arasında Pearson korelasyon testi uygulandı. Çalışmanın sonuçları, gebe ratların tükettiği krill yağı ilaveli yoğurt diyetinin kemik uzunluk parametreleri ve yavrularının biyometrik parametreleri üzerinde olumlu etkileri olduğunu gösterdi.

Keywords

Krill yağı, morfometri, rat, yoğurt

Ethical Statement

Ethical approval was obtained from the Burdur Mehmet Akif Ersoy University Animal Experiments Local Ethics Committee (No: 1046, Date: 07.03.2023) in order to conduct this study.

The Effects of a Diet Containing Yoghurt with Krill Oil Consumed by Rats During Their Pregnancy on Long Bones of Their Offspring

Abstract

The use of calcium, vitamins, minerals, and omega 3 fatty acids during pregnancy are recommended to support the bone development of infants. The aim of this study is to feed pregnant rats with the probiotic yoghurt mixed with krill oil, which is rich in these features, and examine the morphometric development of long bones in their offspring. For this purpose, a total of twelve 2-month-old offspring including 6 in the experimental group (offspring of pregnant rats fed with yoghurt mixed with krill oil) and 6 in the control group (offspring of pregnant rats fed a standard feed), were used in the study. When they became 2 months old, their biometric measurements were taken. After euthanasia, long bones of the offspring (Humerus, antebrachium, femur, ossa cruris) were cleaned by maceration. These bones were photographed. Morphometric measurements of the length and width of these bones were made using the Image J program. In the SPSS (20.0 Version) program, the parameters were compared between the right and left leg bones and between the control and experimental groups by running the Independent Samples T test. Additionally, Pearson’s correlation test was applied between the parameters. The results of the study indicated that this diet with krill oil and yogurt consumed by pregnant rats had positive effects on the length parameters of the bones and biometric parameters of their offspring.

Keywords

Krill oil, Morphometry, Rat, Yoghurt

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