Sıçanlarda Lipopolisakkarit ile Uyarılan Ovaryum Hasarında Timol’ün Hormonal, Antiinflamatuvar ve Antioksidan Yollarla Koruyucu Etkileri

Year 2025, Volume: 20 Issue: 1, 1 - 7, 29.04.2025

İshak Gökçek , Gökhan Uyanık

https://doi.org/10.17094/vetsci.1529099

Abstract

Bu çalışmada LPS ile uyarılan ovaryum hasarında timolün koruyucu etkileri araştırıldı. Bu amaçla Wistar albino dişi sıçanlar beş gruba ayrıldı (n=35, grup=7): kontrol, çözücü, lps, timol, lps+timol. Çalışma sonunda hayvanlardan alınan numunelerde hormon (E2 ve AMH), oksidatif stres (MDA, GSH, GSH-Px ve katalaz), yangısal sitokin (TNF-α ve IL-6) analizleri gerçekleştirildi. Ayrıca ovaryum ve canlı ağırlıklarda da ölçüldü. Lipopolisakkarit uygulaması, E2 AMH, GSH, GSH-Px ve katalaz seviyelerinde azalmalara neden olurken MDA, TNF-α ve IL-6 düzeylerinde artışlara neden oldu. Lps+timol grubunda ise timol uygulaması E2, AMH, GSH, GSH-Px ve katalaz da artışlara, MDA, TNF-α ve IL-6 seviyelerinde ise azalmalara neden olduğu görüldü. Sonuç olarak timol uygulaması antioksidan ve anti-inflamatuvar mekanizmalar üzerinden Lps kaynaklı hormonal, oksidatif stres, yangısal değişiklikleri olumlu olarak etkilediği ancak timolün uzun süreli etkilerinin özellikle daha ileri moleküler mekanizmalarla ortaya konmasına ihtiyaç olduğu düşünülmektedir.

Keywords

LPS , timol , ovaryum rezerv , yangı , oksidan-antioksidan durum

Protective Effects of Thymol with Hormonal, Antiinflammatory and Antioxidant Pathways in Lipopolysaccharide-Induced Ovarian Damage in Rats

Abstract

This study investigated the protective effects of thymol on LPS-induced ovarian damage. Female Wistar albino rats were divided into five groups (n=35, group=7): control, solvent, LPS, thymol, and LPS+thymol. At the end of the study, hormone (E2 and AMH), oxidative stress (MDA, GSH, GSH-Px and catalase), and inflammatory cytokine (TNF-α and IL-6) analyses were performed in the samples taken from the animals. Ovary and body weights were also measured. Lipopolysaccharide treatment caused decreases in E2 AMH, GSH, GSH-Px and catalase levels but increased MDA, TNF-α and IL-6 levels. In the Lps+thymol group, thymol administration caused increases in E2, AMH, GSH, GSH-Px and catalase levels and decreased MDA, TNF-α and IL-6 levels. In conclusion, thymol administration positively affected Lps-induced hormonal, oxidative stress and inflammatory changes via antioxidant and anti-inflammatory mechanisms. However, it is thought that the long-term effects of thymol need to be demonstrated, especially by further molecular mechanisms.

Keywords

LPS , thymol , ovarian reserve , inflammation , oxidant-antioxidant status

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