Sıçanlarda Lipopolisakkarit ile Uyarılan Ovaryum Hasarında Timol’ün Hormonal, Antiinflamatuvar ve Antioksidan Yollarla Koruyucu Etkileri

Yıl 2025, Cilt: 20 Sayı: 1, 1 - 7, 29.04.2025

İshak Gökçek , Gökhan Uyanık

https://doi.org/10.17094/vetsci.1529099

Öz

Bu çalışmada LPS ile uyarılan ovaryum hasarında timolün koruyucu etkileri araştırıldı. Bu amaçla Wistar albino dişi sıçanlar beş gruba ayrıldı (n=35, grup=7): kontrol, çözücü, lps, timol, lps+timol. Çalışma sonunda hayvanlardan alınan numunelerde hormon (E2 ve AMH), oksidatif stres (MDA, GSH, GSH-Px ve katalaz), yangısal sitokin (TNF-α ve IL-6) analizleri gerçekleştirildi. Ayrıca ovaryum ve canlı ağırlıklarda da ölçüldü. Lipopolisakkarit uygulaması, E2 AMH, GSH, GSH-Px ve katalaz seviyelerinde azalmalara neden olurken MDA, TNF-α ve IL-6 düzeylerinde artışlara neden oldu. Lps+timol grubunda ise timol uygulaması E2, AMH, GSH, GSH-Px ve katalaz da artışlara, MDA, TNF-α ve IL-6 seviyelerinde ise azalmalara neden olduğu görüldü. Sonuç olarak timol uygulaması antioksidan ve anti-inflamatuvar mekanizmalar üzerinden Lps kaynaklı hormonal, oksidatif stres, yangısal değişiklikleri olumlu olarak etkilediği ancak timolün uzun süreli etkilerinin özellikle daha ileri moleküler mekanizmalarla ortaya konmasına ihtiyaç olduğu düşünülmektedir.

Anahtar Kelimeler

LPS , timol , ovaryum rezerv , yangı , oksidan-antioksidan durum

Protective Effects of Thymol with Hormonal, Antiinflammatory and Antioxidant Pathways in Lipopolysaccharide-Induced Ovarian Damage in Rats

Öz

This study investigated the protective effects of thymol on LPS-induced ovarian damage. Female Wistar albino rats were divided into five groups (n=35, group=7): control, solvent, LPS, thymol, and LPS+thymol. At the end of the study, hormone (E2 and AMH), oxidative stress (MDA, GSH, GSH-Px and catalase), and inflammatory cytokine (TNF-α and IL-6) analyses were performed in the samples taken from the animals. Ovary and body weights were also measured. Lipopolysaccharide treatment caused decreases in E2 AMH, GSH, GSH-Px and catalase levels but increased MDA, TNF-α and IL-6 levels. In the Lps+thymol group, thymol administration caused increases in E2, AMH, GSH, GSH-Px and catalase levels and decreased MDA, TNF-α and IL-6 levels. In conclusion, thymol administration positively affected Lps-induced hormonal, oxidative stress and inflammatory changes via antioxidant and anti-inflammatory mechanisms. However, it is thought that the long-term effects of thymol need to be demonstrated, especially by further molecular mechanisms.

Anahtar Kelimeler

LPS , thymol , ovarian reserve , inflammation , oxidant-antioxidant status

Kaynakça

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