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JAPON BALIK (Carassius auratus, L. 1758)’LARINDA YEME İLAVE EDİLEN PROBİYOTİK (Lactococcus lactis subsp. Cremoris) VE PREBİYOTİĞİN (Mannanoligosaccharide) Aeromonas hydrophila’ya KARŞI HASTALIK DİRENCİ ÜZERİNE ETKİSİ

Year 2021, Volume: 6 Issue: 1, 49 - 55, 30.07.2021

Abstract

Bu çalışmada probiyotik (Lactococcus lactis subsp. cremoris, LAB), prebiyotik (mannanoligosakarit, MOS) ve simbiyotik (Lactococcus lactis subsp. cremoris + MOS) ile beslemenin Japon balıklarında, Carassius auratus (ortalama ağırlığı 4.32 gr ± 0.68) hastalık direnci üzerindeki etkilerini incelemek için bir beslenme deneyi yapılmıştır. Probiyotik (108 kob/g, LAB), prebiyotik (2 g/kg MOS), simbiyotik (108 kob/g LAB + 2 g/kg MOS) ve kontrol grubunu (yem katkısı yapılmamış) içeren sekiz deneysel diyet hazırlanmış ve balıklar 60 gün süresince beslenmiştir. Besleme denemesi sonunda balıklar LD50 dozunda (2,75x104 kob/ml) A.hydrophila ile enfekte edilmiştir. Ölümler 15 gün boyunca takip edilmiş ve hayatta kalma değerleri hesaplanmıştır. Deneme sonucunda prebiyotik ilavesi ile beslenen gruplarda kontrol grubu balıklarına kıyasla, ölüm oranlarında önemli bir azalma kaydedildiği görülmüştür. Prebiyotik ilaveli yemlerle beslenen balıklarda RPS değeri % 50 olarak hesaplanmıştır. Bununla birlikte, probiyotik (Lactococcus lactis subsp. cremoris) ilaveli diyetlerle beslenen balıklarda ise A.hidrophila'ya karşı koruma elde edilmemiştir. Sonuç olarak, Japon balıklarında Aeromonas hydrophila enfeksiyonuna karşı hastalık direncini arttırmak için mannanoligosakaritin kullanılabileceğini görülmüşütür.

References

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  • Akhter, N., Wu, B., Memon, A. M., Mohsin, M., 2015. Probiotics and prebiotics associated with aquaculture: A review. Fish & Shellfish Immunology, 45(2), 733-741. DOI: 10.1016/j.fsi.2015.05.038
  • Defoirdt, T., Boon, N., Sorgeloos, P., Verstraete, W., Bossier, P., 2007. Alternatives to antibiotics to control bacterial infections: luminescent vibriosis in aquaculture as an example. Trends Biotechnology 25, 472– 479. doi: 10.1016/j.tibtech.2007.08.001
  • Geraylou, Z., Souffreau, C., Rurangwa, E., De Meester, L., Courtin, C.M., Delcour,J.A., Buyse, J., Ollevier, F. 2013. Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immu nity and gut microbiota of juvenile Siberian sturgeon (Acipenser baerii). Fish Shellfish Immunol., 35, 766–775. doi: 10.1016/j.fsi.2013.06.014.
  • Genç, E., M. Genç, A., Aktaş, M., Barcan, Y.Y., İkizdoğan A. T. Su Ürünleri Yetiştiriciliğinde Mannan-oligosakkarit (MOS) Kullanımı Üzerine Türkiye’de Farkındalık Yaratma. Eğirdir Su Ürünleri Fakültesi Dergisi 7(1): 18-24 (2011)
  • Gomez-Gill, B., Roque, A., Turnbull, J.F., 2000. The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture, 191, 259-270. doi:10.1016/S0044-8486(00)00431-2
  • Guzman-Villanueva, L.T., Tovar-Ramirez, D., Gisbert, E., Cordero, H., Guardiola, F.A., Cuesta, A., Meseguer, J., Ascencio-Valle, F., Esteban, M.A., 2014. Dietary administration of b-1,3/1.6-glucan and probiotic strain Shewanella putrefaciens, single or combined, on gilthead seabream growth, immune responses and gene expression. Fish Shellfish Immunol., 39, 34–41. doi: 10.1016/j.fsi.2014.04.024.
  • Hekimoğlu, M. A., 2004. Akvaryum Balıklarının Önemi Ve Sektörün Dünyadaki Ve Türkiye`deki Genel Durumu. Akvaryum Dünyası, 1,(4), 18-19.
  • Hekimoğlu, M. A., 2006. Akvaryum Sektörünün Dünyadaki ve Türkiye’deki Genel Durumu, (1/2): 237-241
  • Hoseinifar, S. H., Mirvaghefi, A., Amoozegar, M. A., Sharifian, M., & Esteban, M. Á. (2015). Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhynchus mykiss) upon synbiotic feeding. Fish & shellfish immunology, 45(1), 27-32. DOI: 10.1016/j.fsi.2015.03.029
  • Irianto, A., Austin, B. 2002. Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 25, 333- 342. https://doi.org/10.1046/j.1365-2761.2002.00375.x
  • Kesarcodi-Watson, A., Kaspar, H., Lategan, M.L.J., Gibson, L., (2008). Probiotics in aquaculture: The need, principles and mechanism of action and screening processes, Aquaculture, 274: 1-14. doi:10.1016/j.aquaculture.2007.11.019
  • Kılıçerkan, M., Çek, Ş., (2011). Hatay ilçelerindeki akvaryum işletmelerinin genel profili’nin çıkarılması üzerine bir araştırma.Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 1(4): 77-82, 2011
  • Merrifield, D.L., Dimitroglou, A., Foey, A., Davies, S.J., Baker, R.T.M., Bøgwald, J., Castex, M., Ringø, E. 2010. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302, 1-18. DOI : 10.1016/j.aquaculture.2010.02.007
  • Öztürk, R. Ç., & Altınok, İ. (2014). Bacterial and viral fish diseases in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 14(1), 275-297. DOI: 10.4194/1303-2712-v14_1_30
  • Reddacliff, G.L., 1988. Disease of aquarium fish. Refresher Course for Veteriniaries. Sydney
  • Reyes-Becerril, M., Ascencio, F., Gracia-Lopez, V., Macias, M. E., Roa, M. C., & Esteban, M. Á. (2014). Single or combined effects of Lactobacillus sakei and inulin on growth, non-specific immunity and IgM expression in leopard grouper (Mycteroperca rosacea). Fish physiology and biochemistry, 40(4), 1169-1180. doi: 10.1007/s10695-014-9913-z.
  • Sakai M. (1999) Current research status of fish immunostimulants. Aquaculture 172, 63-92. https://doi.org/10.1016/S0044-8486(98)00436-0
  • Sapkota, A., Sapkota, A.R., Kucharski, M., Burke, J., McKenzie, S.,Walker, P. and Lawrence, R., 2008. Aquaculture practices and potential human health risks: current knowledge and future priorities. Environ. Int., 34: 1215–1226. doi: 10.1016/j.envint.2008.04.009.
  • Sivaram, V. (2004). Growth and immune response of juvenile greasy groupers (Epinephelus tauvina) fed with herbal antibacterial active principle supplemented diets against Vibrio infections. Article in Aquaculture 237(1-4):9-20 DOI: 10.1016/j.aquaculture.2004.03.014
  • Smith, V.J., Brown, J.H., Hauton, C., 2003. Immunostimulation in crustaceans: does it really protect against infection? Fish Shellfish Immun., 15: 71–90. https://doi.org/10.1016/S1050-4648(02)00140-7
  • Taşdemir, A. (2017). Probiyotikler, Prebiyotikler, Sinbiyotikler. Sağlık Akademisi Kastamonu, 2(1), 71-88.
  • Villamil, L., Reyes, C., Martínez‐Silva, M.A., 2014. In vivo and in vitro assessment of Lactobacillus acidophilus as probiotic for tilapia (Oreochromis niloticus, Perciformes: Cichlidae) culture improvement. Aquacult. Res., 45(7): 1116-1125. https://doi.org/10.1111/are.12051
  • Vine, N.G., Leukes, W.D., Kaiser, H., (2006). Probiotics in marine larviculture, FEMS Mic-robiology Reviews, 30: 404-427. DOI: 10.1111/j.1574-6976.2006.00017.x
  • Wang, Y.B., Li, J.R., Lin, J. 2008. Probiotics in aquaculture: challenges and Outlook. Aquaculture, 281(1),1-4. https://doi.org/10.1016/j.aquaculture.2008.06.002
Year 2021, Volume: 6 Issue: 1, 49 - 55, 30.07.2021

Abstract

References

  • Abid, A., Davies, S. J., Waines, P., Emery, M., Castex, M., Gioacchini, G.,Carnevali, O., Bickerdike, R., Romeo, J. & Merrifield, D. L. (2013). Dietary synbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity. Fish & Shellfish Immunology, 35(6), 1948-1956. https://doi.org/10.1016/j.fsi.2013.09.039
  • Akhter, N., Wu, B., Memon, A. M., Mohsin, M., 2015. Probiotics and prebiotics associated with aquaculture: A review. Fish & Shellfish Immunology, 45(2), 733-741. DOI: 10.1016/j.fsi.2015.05.038
  • Defoirdt, T., Boon, N., Sorgeloos, P., Verstraete, W., Bossier, P., 2007. Alternatives to antibiotics to control bacterial infections: luminescent vibriosis in aquaculture as an example. Trends Biotechnology 25, 472– 479. doi: 10.1016/j.tibtech.2007.08.001
  • Geraylou, Z., Souffreau, C., Rurangwa, E., De Meester, L., Courtin, C.M., Delcour,J.A., Buyse, J., Ollevier, F. 2013. Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immu nity and gut microbiota of juvenile Siberian sturgeon (Acipenser baerii). Fish Shellfish Immunol., 35, 766–775. doi: 10.1016/j.fsi.2013.06.014.
  • Genç, E., M. Genç, A., Aktaş, M., Barcan, Y.Y., İkizdoğan A. T. Su Ürünleri Yetiştiriciliğinde Mannan-oligosakkarit (MOS) Kullanımı Üzerine Türkiye’de Farkındalık Yaratma. Eğirdir Su Ürünleri Fakültesi Dergisi 7(1): 18-24 (2011)
  • Gomez-Gill, B., Roque, A., Turnbull, J.F., 2000. The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture, 191, 259-270. doi:10.1016/S0044-8486(00)00431-2
  • Guzman-Villanueva, L.T., Tovar-Ramirez, D., Gisbert, E., Cordero, H., Guardiola, F.A., Cuesta, A., Meseguer, J., Ascencio-Valle, F., Esteban, M.A., 2014. Dietary administration of b-1,3/1.6-glucan and probiotic strain Shewanella putrefaciens, single or combined, on gilthead seabream growth, immune responses and gene expression. Fish Shellfish Immunol., 39, 34–41. doi: 10.1016/j.fsi.2014.04.024.
  • Hekimoğlu, M. A., 2004. Akvaryum Balıklarının Önemi Ve Sektörün Dünyadaki Ve Türkiye`deki Genel Durumu. Akvaryum Dünyası, 1,(4), 18-19.
  • Hekimoğlu, M. A., 2006. Akvaryum Sektörünün Dünyadaki ve Türkiye’deki Genel Durumu, (1/2): 237-241
  • Hoseinifar, S. H., Mirvaghefi, A., Amoozegar, M. A., Sharifian, M., & Esteban, M. Á. (2015). Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhynchus mykiss) upon synbiotic feeding. Fish & shellfish immunology, 45(1), 27-32. DOI: 10.1016/j.fsi.2015.03.029
  • Irianto, A., Austin, B. 2002. Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 25, 333- 342. https://doi.org/10.1046/j.1365-2761.2002.00375.x
  • Kesarcodi-Watson, A., Kaspar, H., Lategan, M.L.J., Gibson, L., (2008). Probiotics in aquaculture: The need, principles and mechanism of action and screening processes, Aquaculture, 274: 1-14. doi:10.1016/j.aquaculture.2007.11.019
  • Kılıçerkan, M., Çek, Ş., (2011). Hatay ilçelerindeki akvaryum işletmelerinin genel profili’nin çıkarılması üzerine bir araştırma.Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 1(4): 77-82, 2011
  • Merrifield, D.L., Dimitroglou, A., Foey, A., Davies, S.J., Baker, R.T.M., Bøgwald, J., Castex, M., Ringø, E. 2010. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302, 1-18. DOI : 10.1016/j.aquaculture.2010.02.007
  • Öztürk, R. Ç., & Altınok, İ. (2014). Bacterial and viral fish diseases in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 14(1), 275-297. DOI: 10.4194/1303-2712-v14_1_30
  • Reddacliff, G.L., 1988. Disease of aquarium fish. Refresher Course for Veteriniaries. Sydney
  • Reyes-Becerril, M., Ascencio, F., Gracia-Lopez, V., Macias, M. E., Roa, M. C., & Esteban, M. Á. (2014). Single or combined effects of Lactobacillus sakei and inulin on growth, non-specific immunity and IgM expression in leopard grouper (Mycteroperca rosacea). Fish physiology and biochemistry, 40(4), 1169-1180. doi: 10.1007/s10695-014-9913-z.
  • Sakai M. (1999) Current research status of fish immunostimulants. Aquaculture 172, 63-92. https://doi.org/10.1016/S0044-8486(98)00436-0
  • Sapkota, A., Sapkota, A.R., Kucharski, M., Burke, J., McKenzie, S.,Walker, P. and Lawrence, R., 2008. Aquaculture practices and potential human health risks: current knowledge and future priorities. Environ. Int., 34: 1215–1226. doi: 10.1016/j.envint.2008.04.009.
  • Sivaram, V. (2004). Growth and immune response of juvenile greasy groupers (Epinephelus tauvina) fed with herbal antibacterial active principle supplemented diets against Vibrio infections. Article in Aquaculture 237(1-4):9-20 DOI: 10.1016/j.aquaculture.2004.03.014
  • Smith, V.J., Brown, J.H., Hauton, C., 2003. Immunostimulation in crustaceans: does it really protect against infection? Fish Shellfish Immun., 15: 71–90. https://doi.org/10.1016/S1050-4648(02)00140-7
  • Taşdemir, A. (2017). Probiyotikler, Prebiyotikler, Sinbiyotikler. Sağlık Akademisi Kastamonu, 2(1), 71-88.
  • Villamil, L., Reyes, C., Martínez‐Silva, M.A., 2014. In vivo and in vitro assessment of Lactobacillus acidophilus as probiotic for tilapia (Oreochromis niloticus, Perciformes: Cichlidae) culture improvement. Aquacult. Res., 45(7): 1116-1125. https://doi.org/10.1111/are.12051
  • Vine, N.G., Leukes, W.D., Kaiser, H., (2006). Probiotics in marine larviculture, FEMS Mic-robiology Reviews, 30: 404-427. DOI: 10.1111/j.1574-6976.2006.00017.x
  • Wang, Y.B., Li, J.R., Lin, J. 2008. Probiotics in aquaculture: challenges and Outlook. Aquaculture, 281(1),1-4. https://doi.org/10.1016/j.aquaculture.2008.06.002
There are 25 citations in total.

Details

Primary Language Turkish
Journal Section Articels
Authors

Mehmet Tatlı This is me 0000-0002-8259-4898

Seçil Metin 0000-0002-8029-5624

Publication Date July 30, 2021
Submission Date March 11, 2021
Published in Issue Year 2021 Volume: 6 Issue: 1

Cite

APA Tatlı, M., & Metin, S. (2021). JAPON BALIK (Carassius auratus, L. 1758)’LARINDA YEME İLAVE EDİLEN PROBİYOTİK (Lactococcus lactis subsp. Cremoris) VE PREBİYOTİĞİN (Mannanoligosaccharide) Aeromonas hydrophila’ya KARŞI HASTALIK DİRENCİ ÜZERİNE ETKİSİ. Yalvaç Akademi Dergisi, 6(1), 49-55.

http://www.yalvacakademi.org/