Viral Hemorajik Septisemi Virüs Genotip 1e Suşlarının Çipura (Sparus Aurata) ve Levrek (Dicentrarchus Labrax) Balıkları Üzerinde Patojenitelerinin Belirlenmesi

Year 2014, , 0 - 0, 01.04.2014

Hakan Işıdan İlyas Kutlu

https://doi.org/10.17693/yunusae.vi.235396

Abstract

In this study, we have aimed the
determination of susceptibility of sea bass (Dicentrarchus labrax) and sea
bream (Sparus aurata) which they have been cultured in Turkey to viral
haemorrhagic septicemia virus. For this purpose, an infection trial has been conducted
on a number of 360 sea bass and sea bream with using TB13/H15 and SW13/G -5 -1
-5 isolates. The dose of infection applied as 10 TCID ml for infection via
immersion and 10 TCID /fish for 50 50 intraperitoneal infection. As a result of
the infection trial, the average mortality rate for sea bream and sea bass has
been found as 13,3 % and 5 % in the intraperitoneal infections. At the end of
the study, virus was not isolated from dead or alive fish which infected by
immersion. In conclusion, it has been founded that viral haemorrhagic
septicemia virus (Genotype 1e strains) has low pathogenicity on the sea bass
and sea bream.

Keywords

viral hemorrhagic septicemia virus; pathogenicity; infection trial; sea bass, Dicentrarchus labrax; sea

Viral Hemorajik Septisemi Virüs Genotip 1e Suşlarının Çipura (Sparus Aurata) ve Levrek (Dicentrarchus Labrax) Balıkları Üzerinde Patojenitelerinin Belirlenmesi

Abstract

Bu çalışmda, Türkiye'de kültürü yapılan çipura ve levrek balıklarının
ülkemizden izole edilen, viral hemorajik septisemi
virüs genotip 1e suşlarına karşı duyarlılıklarının belirlenmesi amaçlandı. Bu
amaçla TB13/H15 ve SW13/G izolatları ile 360'ar adet
çipura (Sparus aurata) ve Levrek (Dicentrarchus labrax) üzerinde enfeksiyon
denemesi -5 -5 -1 gerçekleştirildi. Enfeksiyon dozu intraperitoneal 10
DKID /balık ve daldırma 10 DKID ml olarak uygulandı. 50 50 Yapılan
enfeksiyon denemesinin sonunda çipuralarda en yüksek mortalite oranı % 13,33
bulunurken levrek balıklarında % 5 olarak bulundu. Ölen ve
hayatta kalan balıklardan hücre kültüründe yapılan ekimler sonunda, daldırma
yoluyla virüs verilen balıkların hiçbirisinden yeniden virüs
izolasyonu yapılamadı. Sonuç olarak yapılan çalışma ile viral hemorajik
septisemi virüsün çipura ve levrek balıklarında düşük patojeniteye sahip olduðu
ortaya konuldu.

Keywords

Viral Hemorajik Septisemi Virüs; patojenite; enfeksiyon denemesi; çipura, Dicentrarchus labrax;

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