Viral Hemorajik Septisemi Virüs Genotip 1e Suşlarının Çipura (Sparus Aurata) ve Levrek (Dicentrarchus Labrax) Balıkları Üzerinde Patojenitelerinin Belirlenmesi

Öz

In this study, we have aimed the determination of susceptibility of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) which they have been cultured in Turkey to viral haemorrhagic septicemia virus. For this purpose, an infection trial has been conducted on a number of 360 sea bass and sea bream with using TB13/H15 and SW13/G -5 -1 -5 isolates. The dose of infection applied as 10 TCID ml for infection via immersion and 10 TCID /fish for 50 50 intraperitoneal infection. As a result of the infection trial, the average mortality rate for sea bream and sea bass has been found as 13,3 % and 5 % in the intraperitoneal infections. At the end of the study, virus was not isolated from dead or alive fish which infected by immersion. In conclusion, it has been founded that viral haemorrhagic septicemia virus (Genotype 1e strains) has low pathogenicity on the sea bass and sea bream.

Anahtar Kelimeler

• viral hemorrhagic septicemia virus; pathogenicity; infection trial; sea bass, Dicentrarchus labrax; sea

Viral Hemorajik Septisemi Virüs Genotip 1e Suşlarının Çipura (Sparus Aurata) ve Levrek (Dicentrarchus Labrax) Balıkları Üzerinde Patojenitelerinin Belirlenmesi

Öz

Bu çalışmda, Türkiye'de kültürü yapılan çipura ve levrek balıklarının ülkemizden izole edilen, viral hemorajik septisemi virüs genotip 1e suşlarına karşı duyarlılıklarının belirlenmesi amaçlandı. Bu amaçla TB13/H15 ve SW13/G izolatları ile 360'ar adet çipura (Sparus aurata) ve Levrek (Dicentrarchus labrax) üzerinde enfeksiyon denemesi -5 -5 -1 gerçekleştirildi. Enfeksiyon dozu intraperitoneal 10 DKID /balık ve daldırma 10 DKID ml olarak uygulandı. 50 50 Yapılan enfeksiyon denemesinin sonunda çipuralarda en yüksek mortalite oranı % 13,33 bulunurken levrek balıklarında % 5 olarak bulundu. Ölen ve hayatta kalan balıklardan hücre kültüründe yapılan ekimler sonunda, daldırma yoluyla virüs verilen balıkların hiçbirisinden yeniden virüs izolasyonu yapılamadı. Sonuç olarak yapılan çalışma ile viral hemorajik septisemi virüsün çipura ve levrek balıklarında düşük patojeniteye sahip olduðu ortaya konuldu.

Anahtar Kelimeler

• Viral Hemorajik Septisemi Virüs; patojenite; enfeksiyon denemesi; çipura, Dicentrarchus labrax;

Kaynakça

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