Investigation of Callipyge Gene Polymorphism in Akkaraman Sheep Breed

Year 2023, , 215 - 219, 30.04.2023

Melike Kavuzkoz , Koray Kırıkçı

https://doi.org/10.53433/yyufbed.1145441

Abstract

Callipyge (CLPG) is a candidate gene with a significant effect on meat quality and growth in sheep. The aim of this study was to investigate the polymorphism of the CLPG gene in the Akkaraman breed. In the study, DNAs obtained from the blood samples of 50 Akkaraman sheep was used as study material. The CLPG/Faq1 polymorphism was examined in all individuals using the method PCR-RFLP. Only A allele and AA genotype were detected after PCR-RFLP procedure. This is the first study in which CLPG gene polymorphism was investigated in Akkaraman breed, and the findings showed that the Akkaraman breed has a monomorphic structure in terms of CLPG/FaqI polymorphism.

Keywords

Akkaraman sheep, Callipyge, FaqI, PCR-RFLP

Thanks

The author would like to thank the General Directorate of Agricultural Research and Policy of the Ministry of Agriculture and Forestry and the Yozgat Provincial Sheep Breeders Association and the technical staff of the project for their support.

Akkaraman Koyun Irkında Callipyge Gen Polimorfizmin Araştırılması

Abstract

Callipyge (CLPG) koyunlarda et kalitesi ve büyüme üzerinde önemli etkisi olan bir aday gendir. Çalışmada Akkaraman koyun ırkında CLPG gen polimorfizmin belirlemesi amaçlanmıştır. Araştırmada Akkaraman ırkı 50 baş koyuna ait kan örneklerinden elde edilen DNA’lar çalışma materyali olarak kullanılmıştır. Tüm bireylerde CLPG/FaqI polimorfizmi PCR-RLP yöntemi ile araştırılmıştır. PCR-RFLP işlemi sonrasında sadece A alleli ve AA genotipi tespit edilmiştir. Bu çalışma CLPG gen polimorfizmin Akkaraman ırkında araştırıldığı ilk çalışma olup elde edilen bulgular Akkaraman ırkının CLP/Faq1 polimorfizmi bakımından monomorfik bir yapıya sahip olduğunu göstermiştir.

Keywords

Akkaraman koyunu, Callipyge, FaqI, PCR-RFLP

References

• Balcıoğlu, M., Karslı, T., Şahin, E., Ulutaş, Z., & Aksoy, Y. (2014). Türkiye'de yetiştirilen bazı yerli koyun ırklarında kalpastatin (CAST) geni polimorfzminin PCR-RFLP yöntemiyle belirlenmesi. Tarım Bilimleri Dergisi, 20(4), 427-433. doi:10.15832/tbd.87050
• Bayram, D., Akyüz, B., Arslan, K., Özdemir, F., Aksel, E. G., & Çınar, M. U. (2019). DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Universitesi Veteriner Fakultesi Dergisi, 25(1), 9-15. doi:10.9775/kvfd.2018.20055
• Bozhilova-Sakova, M., Dimitrova, I., Tzonev, T. I., & Petrov, N. (2020). Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science, 26(2), 475-479.
• Cesarani, A., Gaspa, G., Correddu, F., Cellesi, M., Dimauro, C., & Macciotta, N. P. P. (2019). Genomic selection of milk fatty acid composition in Sarda dairy sheep: Effect of different phenotypes and relationship matrices on heritability and breeding value accuracy. Journal of Dairy Science, 102(4), 3189-3203. doi:10.3168/jds.2018-15333
• Ceyhan, A., Şekeroğlu, A., & Duman, M. (2019). Some reproductive traits and lambs growth performance of Akkaraman sheep raised in Niğde province. Turkish Journal of Agriculture-Food Science and Technology, 7(10), 1509–1514. doi:10.24925/turjaf.v7i10.1509-1514.2249
• Cockett, N. E., Jackson, S. P., Shay, T. L., Nielsen, D., Moore, S. S., Steele, M. R., Barendse, W., Green, R. D., & Georges, M. (1994). Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proceedings of the National Academy of Sciences, 91(8), 3019-3023. doi.org/10.1073/pnas.91.8.301
• Cockett, N. E., Jackson, S. P., Shay, T. L., Farnir, F., Berghmans, S., Snowder, G. D., Neilsen, D. M., & Georges, M. (1996). Polar overdominance at the ovine callipyge locus. Science, 273(5272), 236-238. doi:10.1126/science.273.5272.236
• Esen, V. K., Esen, S., Karadağ, O., Önenç, A., & Elmacı, C. (2022a). Genotypic characterization of meat-type lambs expressing the callipyge gene in Turkey: I. Carcass characteristics and retail yield. Turkish Journal of Veterinary & Animal Sciences, 46(1), 157-164. doi:10.3906/vet-2112-7
• Esen, V. K, Esen, S., Karadağ, O., & Elmacı, C. (2022b). Genotypic characterization of meat-type lambs expressing the callipyge gene in Turkey: II. Effect on body indexes. Small Ruminant Research, 208, 106633. doi:10.1016/j.smallrumres.2022.106633
• Gootwine, E., Rosov, A., Bor, A., Yossafi, S., & Zenue, A. (2003). Introgression of the callipyge mutation into the Assaf fat tail breed. Options Méditerranéennes, 55, 125-131.
• Gorlov, I. F., Shirokova, N. V., Kolosov, Y. A., Kolosov, A. Y., Getmantseva, L. V., Slozhenkina, M. I., … & Ponomariov, V. V. (2020). Polymorphism of CLPG gene in three sheep breeds grown in the steppe zone of the Russian Federation. Journal of Advanced Veterinary and Animal Research, 7(1), 51. doi:10.5455/javar.2020.g392
• Jackson, S. P., Green, R. D., & Miller, M. F. (1997). Phenotypic characterization of Rambouillet sheep expressing the callipyge gene: I. Inheritance of the condition and production characteristics. Journal of Animal Science, 75(1), 14-18. doi:10.2527/1997.75114x
• Jawasreh, K. I., Al-Amareen, A. H., & Abdullah, A. Y. (2016). P7000 Growth performance and meat characteristics of Awassi sheep that holds the callipyge gene. Journal of Animal Science, 94(4), 175-176. doi:10.2527/jas2016.94supplement4175b
• Jawasreh, K. I., Alamareen, A. H., Obeidat, M. D., & Aad, P. Y. (2019). Growth performance and meat characteristics of the first awassi–rambouillet callipyge backcross. Animals, 9(8), 517. doi:10.3390/ani9080517
• Kırıkçı, K., Çam, M. A., & Mercan, L. (2021). Investigation of the CAST Gene Polymorphism in Karayaka Sheep. Manas Journal of Agriculture Veterinary and Life Sciences, 11(1), 89-93.
• Kırıkçı, K. (2022). Polymorphism of the calpastatin (CAST) and growth differentiation factor 9 (GDF9) genes in Akkaraman Sheep Breed. Journal of Animal Production, 63(1), 21-26. doi:10.29185/hayuretim.1008768
• Koohmaraie, M., Shackelford, S. D., Wheeler, T. L., Lonergan, S. M., & Doumit, M. E. (1995). A muscle hypertrophy condition in lamb (Callipyge): Characterization of effects on muscle growth and meat quality traits. Journal of Animal Science, 73(12), 3596–3607. doi:10.2527/1995.73123596x
• Meena, A. S., Kumari, R., Jyotsana, B., Kumar, R., Prince, L. L. L., Kumar, V., & Kumar, S. (2018). Absence of overdominance phenotype of Callipyge gene in Indian sheep. Indian Journal of Animal Sciences, 88(4), 504–505.
• Mohammed, M. M., Al-Thuwaini, T. M., & Al-Shuhaib, M. B. S. (2022). A novel p.K116Q SNP in the OLR1 gene and its relation to fecundity in Awassi ewes. Theriogenology, 184, 185-190. doi:10.1016/j.theriogenology.2022.03.014
• Nanekarani, S., Goodarzi, M., & Mahdavi, M. (2014). Analysis of polymorphism of Callipyge Gene in Lori Sheep by PCR-RFLP method. APCBEE Procedia, 8, 65-69. doi:10.1016/j.apcbee.2014.03.002
• Penick, M., Kim, H. W., Setyabrata, D., Waddell, J. N., Bidwell, C. A., & Kim, Y. H. B. (2017). Callipyge genotypic effects on meat quality attributes and oxidation stability of ovine M. longissimus. Small Ruminant Research, 146, 5-12. doi:10.1016/j.smallrumres.2016.11.011
• Ünal, N. (2002). Survival rate, growth characteristics and some body measurements of Akkaraman and Sakız (Chios) x Akkaraman F1 lambs. Turkish Journal of Veterinary and Animal Sciences, 26(1), 109-116.