Investigation of Callipyge Gene Polymorphism in Akkaraman Sheep Breed
Öz
Callipyge (CLPG) is a candidate gene with a significant effect on meat quality and growth in sheep. The aim of this study was to investigate the polymorphism of the CLPG gene in the Akkaraman breed. In the study, DNAs obtained from the blood samples of 50 Akkaraman sheep was used as study material. The CLPG/Faq1 polymorphism was examined in all individuals using the method PCR-RFLP. Only A allele and AA genotype were detected after PCR-RFLP procedure. This is the first study in which CLPG gene polymorphism was investigated in Akkaraman breed, and the findings showed that the Akkaraman breed has a monomorphic structure in terms of CLPG/FaqI polymorphism.
Anahtar Kelimeler
Akkaraman sheep, Callipyge, FaqI, PCR-RFLP
Teşekkür
The author would like to thank the General Directorate
of Agricultural Research and Policy of the Ministry of
Agriculture and Forestry and the Yozgat Provincial
Sheep Breeders Association and the technical staff of
the project for their support.
Kaynakça
- Balcıoğlu, M., Karslı, T., Şahin, E., Ulutaş, Z., & Aksoy, Y. (2014). Türkiye'de yetiştirilen bazı yerli koyun ırklarında kalpastatin (CAST) geni polimorfzminin PCR-RFLP yöntemiyle belirlenmesi. Tarım Bilimleri Dergisi, 20(4), 427-433. doi:10.15832/tbd.87050
- Bayram, D., Akyüz, B., Arslan, K., Özdemir, F., Aksel, E. G., & Çınar, M. U. (2019). DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Universitesi Veteriner Fakultesi Dergisi, 25(1), 9-15. doi:10.9775/kvfd.2018.20055
- Bozhilova-Sakova, M., Dimitrova, I., Tzonev, T. I., & Petrov, N. (2020). Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science, 26(2), 475-479.
- Cesarani, A., Gaspa, G., Correddu, F., Cellesi, M., Dimauro, C., & Macciotta, N. P. P. (2019). Genomic selection of milk fatty acid composition in Sarda dairy sheep: Effect of different phenotypes and relationship matrices on heritability and breeding value accuracy. Journal of Dairy Science, 102(4), 3189-3203. doi:10.3168/jds.2018-15333
- Ceyhan, A., Şekeroğlu, A., & Duman, M. (2019). Some reproductive traits and lambs growth performance of Akkaraman sheep raised in Niğde province. Turkish Journal of Agriculture-Food Science and Technology, 7(10), 1509–1514. doi:10.24925/turjaf.v7i10.1509-1514.2249
- Cockett, N. E., Jackson, S. P., Shay, T. L., Nielsen, D., Moore, S. S., Steele, M. R., Barendse, W., Green, R. D., & Georges, M. (1994). Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proceedings of the National Academy of Sciences, 91(8), 3019-3023. doi.org/10.1073/pnas.91.8.301
- Cockett, N. E., Jackson, S. P., Shay, T. L., Farnir, F., Berghmans, S., Snowder, G. D., Neilsen, D. M., & Georges, M. (1996). Polar overdominance at the ovine callipyge locus. Science, 273(5272), 236-238. doi:10.1126/science.273.5272.236
- Esen, V. K., Esen, S., Karadağ, O., Önenç, A., & Elmacı, C. (2022a). Genotypic characterization of meat-type lambs expressing the callipyge gene in Turkey: I. Carcass characteristics and retail yield. Turkish Journal of Veterinary & Animal Sciences, 46(1), 157-164. doi:10.3906/vet-2112-7
- Esen, V. K, Esen, S., Karadağ, O., & Elmacı, C. (2022b). Genotypic characterization of meat-type lambs expressing the callipyge gene in Turkey: II. Effect on body indexes. Small Ruminant Research, 208, 106633. doi:10.1016/j.smallrumres.2022.106633
- Gootwine, E., Rosov, A., Bor, A., Yossafi, S., & Zenue, A. (2003). Introgression of the callipyge mutation into the Assaf fat tail breed. Options Méditerranéennes, 55, 125-131.