Prokaryotic Diversity of a Hypersaline Spring Water in Pülümür (Tunceli)
Abstract
The microbial diversity of the solar salterns and salt lakes has been extensively studied, but there are only a few reports on microbiota of hypersaline spring waters. In this study, we focused on the archaeal and bacterial groups inhabiting a hypersaline spring water in Pülümür. In addition to culture-dependent studies, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and fluorescence in situ hybridization (FISH) analyzes were performed. The total cell count performed using 4',6-diamidino-2-phenylindole (DAPI) stain revealed ~105 cells mL-1 in spring water. The FISH analysis showed that bacterial cells accounted for ~72% of the total microbial community, while archaeal cells made up a small portion of the community. Isolates were phylogenetically related with the genera Rhodovibrio, Spiribacter, Salinivibrio, Halomonas, Idiomarina, Marinobacter, Natrinema and Halohasta. Phylotypes of Spiribacter and phylotypes distantly associated with Owenweeksia were obtained from DGGE analysis.
Keywords
Hypersaline environments, Halophilic microorganisms, DGGE, FISH
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