Identification and Characterization of Fluorescent Pseudomonas Producing Active Compounds Controlling Plant Pathogens

Abstract

Fluorescent pseudomonad is one of the biocontrol agents against plant pathogens. Various compounds reportedly can be produced by fluorescent pseudomonad, including chitinase, β-1,3-glucanase, HCN, siderophore, antibiotics, Indole Acetic Acid (IAA), phosphate solvent compounds, and 2,4-diacetylphloroglucinol (DAPG). In this study, it was identified and characterized six isolates of fluorescent pseudomonad (PfPj1, PfPj2, PfKd7, PfCas, PfCas3, and LAHp2). All isolates were isolated from the rhizosphere of various types of plants. The results showed that six isolates were identical to Pseudomonas aeruginosa (93-94%). All bacterial isolates tested were able to produce siderophore, HCN, and solubilize phosphates. The highest siderophore was produced by isolate PfPj2. Whereas isolate PfKd7 had the highest at HCN production and the ability to dissolve phosphates.

Keywords

• Siderophore
• HCN
• Dissolving phosphate
• Rhizosphere

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