Characterization of Nepalese Bread Wheat Landraces Based on Morpho-Phenological and Agronomic Traits

Abstract

Due to the presence of valuable genes that contribute to a variety of functional traits, landraces kept in Genebank can be extremely important in wheat breeding. A study was conducted based on agro-morphological traits of Nepalese bread wheat landraces to explore genetic diversity among them. Using a replicated rod row design, 200 landraces were evaluated during the winter season of 2018 and 2019 at Khumaltar, Lalitpur, Nepal. The degree of variations among landraces was determined using univariate and multivariate statistical tools. The Shannon-Weaver diversity index (H’) showed a wide range of variations among the studied landraces, ranging from 0.55 to 0.91 in quantitative traits and 0.63 to 0.85 in qualitative traits.Principal component (PC) analysis with an eigenvalue greater than 1 reveals that 68% of the variability for quantitative traits is contributed by the first five principal components whereas 67% of the variability of qualitative traits is governed by the first four principal components. UPGMA (Unweighted pair-groups methods through arithmetic average) clustered 202 landraces into five groups according to quantitative charterers.Identified advantageous adaptive traits through the analysis of variability within the accessions, will be used by breeders for crosses in the breeding or used directly by farmers.

Keywords

• Genetic diversity
• quantitative traits
• wheat landraces

References

1. Ali, F., Nadeem, M. A., Khalil, I. H., Barut, M., Yüce, İ., & Baloch, F. S. (2022). Estimation of genetic parameters in F3 segregating spring wheat populations for yield and yield related traits. Yuzuncu Yil University Journal of Agricultural Sciences, 32(1), 1-10. https://doi.org/10.29133/yyutbd.886006.
2. Aparicio, N., Villegas, D., Araus, J. L., Blanco, R., & Royo, C. (2002). Seedling development and biomass as affected by seed size and morphology in durum wheat. The Journal of Agricultural Science, 139(2), 143-150.
3. Beral, A., Rincent, R., Le Gouis, J., Girousse, C., & Allard, V. (2020). Wheat individual grain-size variance originates from crop development and from specific genetic determinism. PLoS ONE, 15(3): e0230689. https://doi.org/10.1371/journal.pone.0230689.
4. Borner, A., Schafer, M., Schmidt, A., Grau, M., &Vorwald, J. (2005). Associations between geographical origin and morphological characters in bread wheat (Triticum aestivum L.). Plant Genetic Resources, 3(3), 360–372. https://doi.org/10.1079/PGR200589
5. Bruening, W. P. (2019). Effect of Awns on wheat yield and agronomic characteristics evaluated in variety trials. Journal of Crop Variety Testing, 2,1-5
6. BWRP. (2017). Annual Report 2073/74 (2016/17). National wheat research program, NARC, Bhairahawa, Rupendehi, Nepal.
7. FAOSTAT. (2022). Food and Agriculture organization of the United Nations. Statistical database 2022.
8. Genebank. (2018). Annual Report 2074/75 (2017/18). National Agriculture Genetic Resources Center (Genebank), NARC, Khumaltar, 2018.

9. Gharib, M.A., Qabil, N., Salem, A.H., Ali, M.M., Awaad, H.A., & Mansour, E. (2021). Characterization of wheat landraces and commercial cultivars based on morpho-phenological and agronomic traits. Cereal Research Communications, 49, 149-159. https://doi.org/10.1007/s42976-020-00077-2.
10. Ghimire, K.H., & Magar, R.T. (2017). Diversity in Nepalese wheat genetic resources as revealed by agro-morphological markers. International Journal of Scientific and Engineering Research, 8(7), 1646-1651.
11. IPGRI. (1995). Revised descriptor list for wheat. International Plant Genetic Resources Institute, Rome, Italy.
12. Joshi, B. K., Gorkhali, N. A., Pradhan, N., Ghimire, K. H., Gotame, T. P., KC, P., Mainali, R. P., Karkee, A., & Paneru, R. B. (2020). Agrobiodiversity and its conservation in Nepal. Journal of Nepal Agriculture Research Council, 6,14-33.
13. Karkee, A., Ghimire, K. H., & Joshi, B. K. (2020). Evaluation of Naked barley landraces for Agro-morphological Traits. Journal of Nepal Agricultural Research Council, 6,34-43.
14. Karkee, A., Magar, P. B., Ansari, A. R., Basnet, R., Mandal, D. L., & Budhathok, D. (2019). Wheat diseases observation in wheat diseases screening nursery at agriculture research station, Pakhribas, Dhankuta during 2015 and 2016. Proceedings of 30th national winter crops workshop held in Khumaltar, Lalitpur in 15-16 February 2017.
15. Karkee, A., Mainali, R. P., Basnet, S., Ghimire, K. H., Joshi, B. K., Thapa, P., Shrestha, D. S., Joshi, P., Pokhrel, P., & Mishra, K. K. (2021). Agro-Morphological characterization and intra-varietal diversity of Akabarechilli (Capsicum spp.) landraces of Nepal. SAARC Journal of Agriculture, 19(2), 37-55.
16. Kehel, Z., Sanchez-Garcia, M., El Baouchi, A., Aberkane, H., Tsivelikas, A., Charles, C., & Amri, A. (2020). Predictive characterization for seed morphometric traits for genebank accessions using genomic selection. Frontiers in Ecology and Evolution, 8, 32.https://doi.org/10.3389/fevo.2020.00032
17. Khadka, K., Torkamaneh, D., Kaviani, M., Belzile, F., Raizada, M. N., &Navabi, A. (2020). Population structure of Nepali spring wheat (Triticum aestivum L.) germplasm. BMC Plant Biology, 20, 530. https://doi.org/10.1186/s12870-020-02722-8
18. Kiyoshi, F., Tsuji, T., Yoshida, T., Funatsuki, W. M., & Ikeda, T. M. (2011). Application of ‘glume color’ controlled by Rg-B1 locus as a field-marker to detect Glu-B3 alleles encoding low-molecular-weight glutenin sub units in common wheat. Breeding Science, 61,11-16. https://doi.org/10.1270/jsbbs.61.11.
19. Laino, P., Limonta, M., Gerna, D., &Vaccino, P. (2015). Morpho-physiological and qualitative traits of a bread wheat collection spanning a century of breeding in Italy. Biodiversity Data Journal, 3, e4760. https://doi.org/10.3897/DBJ.3.e4760.
20. Levy, A. A., & Feldman, M. (2022). Evolution and origin of bread wheat. The Plant Cell, koac130. https://doi.org/10.1093/plcell/koac130.
21. Lopes, M. S., El-Basyoni, I., Baenziger, P. S., Singh, S., Royo, C., Ozbek, K., Aktas, H., Ozer, E., Ozdemir, F., Manickavelu, A., & Ban, T. (2015). Exploiting genetic diversity from landraces in wheat breeding for adaptation to climate change. Journal of experimental botany, 66(12), 3477-3486.
22. Mainali, R. P., Karkee, A., Neupane, D., Pokhrel, P., Thapa, P., Ghimire, K. H., Joshi, B. K., & Mishra, K. K. (2020). Collaborative exploration and collection of native plant genetic resources as assisted by agrobiodiversity fair. Journal of Agriculture and Natural Resources, 3(2), 67-81.
23. Manohara, K. K., Bhosle, S. P., & Singh, N. P. (2019). Phenotypic diversity of rice landraces collected from Goa state for salinity and agro-morphological traits. Agricultural Research, 8(1), 1-8.
24. Manzano, A. R., Nodals, A. A. R., Gutiérrez, M. I. R., Mayor, Z. F., & Alfonso, L. C. (2001). Morphological and isoenzyme variability of taro (Colocasia esculenta L. Schott) germplasm in Cuba. Plant genetic resources newsletter, 31-40.
25. MINITAB. (2017). Minitab 17 Statistical Software. State College, Pennsylvania: Minitab, Inc. www.minitab.com.
26. MoALD. (2021). Agriculture and Livestock Diary 2079. Ministry of Agriculture and Livestock Development, Agriculture Information and Training Center, Hariharbhawan, Lalitpur, Nepal.
27. Nachimuthu, V. V., Robin, S., Sudhakar, D., Raveendran, M., Rajeswari, S., &Manonmani, S. (2014). Evaluation of rice genetic diversity and variability in a population panel by principal component analysis. Indian Journal of Science and Technology, 7(10), 1555-1562.
28. Rebetzke, G. J., Bonnett, D. G., & Reynolds, M. P. (2016). Awns reduce grain number to increase grain size and harvestable yield in irrigated and rainfed spring wheat. Journal of Experimental Botany, 67(9), 2573-2586.
29. Reynolds, M., Skovmand, B., Trethowan, R., & Pfeiffer, W. (1999). Evaluating a conceptual model for drought tolerance. In: Ribaut JM (ed.) Using molecular markers to improve drought tolerance. Mexico: CIMMYT, 49– 53
30. Ringlund, K., & Everson, E. H. (1968). Leaf pubescence in common wheat, Triticum aestivum L., and resistance to the cereal leaf beetle, Oulema melanopus (L.). Crop Science, 8(6), 705-710.
31. Robbana, C., Kehel, Z., Ben Naceur, M. B., Sansaloni, C., Bassi, F., & Amri, A. (2019). Genome-wide genetic diversity and population structure of Tunisian durum wheat landraces based on DArTseq technology.International Journal of Molecular Sciences, 20(6),1352.
32. Routray, P., Basha, O., Garg, M., Singh, N. K., & Dhaliwal, H. S. (2007). Genetic diversity of landraces of wheat (Triticum aestivum L.) from hilly areas of Uttaranchal, India.Genetic Resources and Crop Evolution,54(6), 1315-1326.
33. Sears, E. R. (1952). Homoeologous chromosomes in Triticumaestivum. Genetics, 37, 624.
34. Si, H., Zhao, M., He, F., & Ma, C. (2013). Effect of Glu-B3 allelic variation on Sodium Dodecyl Sulfate sedimentation volume in common wheat (Triticum aestivum L.). The Scientific World Journal, 5. https://doi.org/10.1155/2013/848549.
35. Singh, S. P., & Dwivedi, V. K. (2002). Genetic divergence in wheat (Triticum aestivum L.). New Agriculturist,13(1-2), 5-7
36. Smith, S. E., Doss, A. A., & Warburton, M. (1991). Morphological and agronomic variation in North African and Arabian alfalfas. Crop Science, 31, 1159-1163.
37. Thapa, P., Manali, R. P., Karkee, A., Ghimire, K. H., Joshi, B. K., & Mishra, K. K. (2021). Characterization and diversity assessment of Nepalese garlic (Allium sativum L.). Journal of Agriculture and Environment, 22, 80-92.
38. Trethowan, R. M., Reynolds, M. P., Skovmand, B., & van Ginkel, M. (1998). The effect of glume pubescence on floret temperature in wheat. Agronomy Abstracts, 18 –22.
39. Upadhyaya, H. D., Sharma, S., Dwivedi, S. L., & Singh, S. K. (2014). Sorghum genetic resources: Conservation and diversity assessment for enhanced utilization in sorghum improvement. Genetics, genomics and breeding of sorghum. CRC Press, Taylor and Francis Group, Boca Raton (USA), London (UK), New York (USA), pp. 28-55.
40. Yadav, M., Sah, S. K., Regmi, A. P., & Marahatta, S. (2020). Assessment of site-specific nutrient management on the productivity of wheat at Bhairawawa, Nepal. Journal of Agriculture and Forestry University, 4,77-82.
41. Yatung, T., Dubey, R.K., Singh, V. & Upadhyay, G. (2014). Geneticdiversity of chilli (Capsicum annuum L.) genotypes of Indiabased on morpho-chemicaltraits. Australian Journal of Crop Science, 8(1): 97-102
42. Yüce, İ., Başkonuş, T., Dokuyucu, T., Akkaya, A., Güngör, H., & Dumlupınar, Z. (2022). Evaluation of quality and some agronomic traits of some advanced bread wheat (Triticum aestivum L.) lines and cultivars under Kahramanmaraş ecological conditions. Yuzuncu Yil University Journal of Agricultural Sciences 32 (2): 362-371. DOI: https://doi.org/10.29133/yyutbd.1060036