Screening of Bean Genotypes Against Bean Common Mosaic Virus (BCMV) by Artificial Inoculation and Molecular Confirmation

Abstract

Bean mosaic virus (BCMV) is a widespread plant pathogen that causes significant bean yield losses in several bean-growing regions worldwide. The use of resistant common bean varieties to BCMV is considered the most efficient and feasible approach to control its effects. Numerous genes and molecular markers associated with resistance to these pathogens have been discovered and used extensively in breeding studies around the world. Screening bean genotypes for resistance to these viruses is a critical step in developing resistant varieties. The goals of the study are to identify virus sources in the region and artificially inoculate Lake Van basin bean genotypes with BCMV. The recovered BCMV strain NL-4 was inoculated with 45 bean cultivars, most of which originated from the Lake Van basin in Turkey. Differentiation between resistant and susceptible was based on visual symptoms, and of the 45 genotypes, 29 were found to be resistant to NL-4, while 16 genotypes were susceptible (8 of them moderately susceptible and 8 of them highly susceptible).

Keywords

• BCMV
• Beans
• Inoculation
• Molecular
• Virus.

References

1. Acikgoz, Z., & Citri A. (1986). Incidence, epidemiology, and identification of viruses on Phaseolus vulgaris L. of Turkey. Turkish Phytopathol, 15, 61-76.
2. Basavaraja, T., Manjunatha, L., Chandora, R., Singh, M., Rathod, S., Dubey, V., Kanishka R. C., Sing, F., Singh, N. P. (2022). Assessment of phenotypic diversity and multi-locational screening against bean common mosaic virus (BCMV) disease resistance in dry bean (Phaseolus vulgaris L.) germplasm. Plant Genetic Resources, 1-8.
3. Bashir, M., Ahmad, Z., & Murata, N. (2000). Seed Borne Viruses: Detection, Identification and Control. Bewap Plaza (PK): Agha Jee Printers, 156.
4. Bhadramurthy, V., & Bhat, A. I. (2009). Biological and molecular characterization of Bean common mosaic virus associated with vanilla in India. Indian Journal of Virology, 20(2), 70-77.
5. Bilge, D., Akköprü, A., Çakmakcı, Ö., Şensoy, S. (2019). Investigation of effects of some root bacteria on common bean (Phaseolus vulgaris L.) plants grown on salt stress. III. Eurasian Agriculture and Natural Sciences Congress, Antalya, Turkey, 17-20 Ekim 2019, 424-436.
6. CGIAR (2015). Common Bean Factsheet, Consultative Group on International Agricultural, http://www.cgiar.org/our-strategy/cropfactsheets/beans, 15.06. 2015.
7. Deligöz, İ., & Sökmen, M. A. (2013). Determination of resistance to bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods. Bitki Koruma Bülteni, 53(2), 101-113.
8. Ekincialp, A., & Şensoy, S. (2018). Phenotypic and molecular determination of anthracnose disease resistance in Lake Van Basin's bean genotypes (Phaseolus vulgaris L.). Legume Research-An International Journal, 41(1), 135-142.

9. Erdinc, C., Turkmen, O., Dasgan, H. Y., & Sensoy, S. (2017a). Phenotypic and molecular genetic diversity among some Turkish bean genotypes. JAPS: Journal of Animal & Plant Sciences, 27(6).
10. Erdinc, C., Turkmen, O., Demir, S., & Sensoy, S. (2017b). Determination of the anthracnose (Colletotrichum lindemuthianum (Sacc. and Magn.) Lambs. Scrib.) resistance in some Turkish bean genotypes by artificial inoculation and molecular methods. JAPS: Journal of Animal & Plant Sciences, 27(1).
11. Erdinc, C., Ekincialp, A., Akköprü, A., Yildiz, M., & Şensoy, S. (2018). Evolution of common beans collected from lake van basin for their resistance to the common bacterial blight (Xanthomonas axonopodis pv. phaseoli). Applied Ecology and Environmental Research, 16(4), 5181-5191.
12. Hall, R. (1991). Compendium of Bean Diseases. St Paul (MN): APS Press Publishers: 102.
13. Hatipoğlu, M., Şensoy, S. (2022). Validity Determination of Some Molecular Markers Used in Melon Breeding. Yuzuncu Yıl University Journal of Agricultural Sciences, 32(2), 424-435.
14. Hegay, S., (2013). Diversity of beans grown in Kyrgyzstan and marker-aided breeding for resistance to bean common mosaic virus and anthracnose. Doctoral Thesis Faculty of Landscape Planning, Horticulture and Agricultural Science Department of Plant Breeding Swedish University of Agricultural Sciences.
15. Ibrahim, A. S., Erdinç, Ç., 2020. Determination of Genetic Relations among Tomato Accessions in Sulaymaniyah Region through ISSRs Markers Genetic Relations in Tomato Accessions. Yuzuncu Yil University Journal of Agricultural Science, 30(4), 31-12.
16. Kaiser, W. J., Danesh, D., Okhovat, M., & Mossahebi, G. H. (1967). Regional pulse improvement project; Diseases of pulses crop (edible legumes) occurring in Iran. Iranian J Plant Pathol, 4, 10-14.
17. Kaiser, W. J., Mossahebi, G. H., & Okhovat, M. (1971). Alternate hosts of viruses affecting food legum es in Iran. Iran J Plant Pathol, 7(2), 85-93.
18. Kelly, J. D., Afanador, L., & Haley, S. D. (1995). Pyramiding genes for resistance to bean common mosaic-virus. Euphytica, 82(3), 207-212.
19. Kıpçak, S., Ekincialp, A., Erdinç, Ç., Kabay, T., & Şensoy, S. (2019). Tuz Stresinin Farklı Fasulye Genotiplerinde Bazı Besin Elementi İçeriği ile Toplam Antioksidan ve Toplam Fenol İçeriğine Etkisi. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(1), 136-144.
20. Klein, R. E., Wyatt, S. D., & Kaiser, W. J. (1988). Incidence of bean common mosaic virus in USDA Phaseolus germplasm collection. Plant Disease, 72, 301-302.
21. Loebenstein, G., & Thottappilly, G. (2004). Virus and virus-like diseases of major crops in developing countries. Dordrecht: Kluwer Academic Publishers, 840.
22. Makkouk, K. M., & Kumari, S. G. (1996). Detection of ten viruses by the tissue blot immunoassay (TBIA). Arab J Plant Prot, 14, 3-9.
23. Makkouk, K. M., Kumari, S. G., Shahraeen, N., Fazlali, Z., Farzadfar, S. H., Ghotbi, T., & Mansouri, A. (2003). Identification and seasonal variation of viral diseases of chickpea and lentil in Iran. J Plant Dis Prot, 110(2), 157-169.
24. Mangeni, B. C., Abang, M. M., Awale, H., Omuse, C. N., Leitch, R., Arinaitwe, W., Mukoye, B., Kelly, J. D., & Were, H. K. (2014). Distribution and pathogenic characterization of bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in western Kenya. Journal of Agri-Food and Applied Sciences, 2(10), 308-316.
25. Mehraban, A. H., Shahraeen, N., Ghotbi, T., & Mostaed, M. (2002). Identification of Bean Common Mosaic Virus (BCMV), Bean Yellow Mosaic Virus (BYMV) and Cucumber Mosaic Virus (CMV) in Markazi and Lorestan Provinces. Paper presented at the 15th Iranian Plant Protection Congress. Razi University, Kermanshah, Iran, 166.
26. Melgarejo, T. A., Lehtonen, M. T., Fribourg, C. E., Rannali, M., & Valkonen, J. P. T. (2007). Strains of BCMV and BCMNV characterized from lima bean plants affected by deforming mosaic disease in Peru. Arch. Virol., 152, 1941-1949.
27. Miklas, P. N., Kelly, J. D., Beebe, S. E., & Blair, M. W. (2006). Common bean breeding for resistance against biotic and abiotic stresses: from classical to MAS breeding. Euphytica, 147, 105-131.
28. Morales, F. J., & Bos, L. (1988). Bean common mosaic virus. AAB descrip plant viruses, 337.
29. Sehirali, S. (1988). Yemeklik Dane Baklagiller, Ankara Üniversitesi Ziraat Fakültesi Yayınları, 1089, Ders Kitabı, Ankara, 314s.
30. Sengooba, T. N., Spence, N. F., Walket, D. G. A., Allen, D. J., & Femi-Lana, A. (1997). The occurrence of bean common mosaic necrosis virus in wild and forage legumes in Uganda. Plant Pathology, 46, 95-103.
31. Shahraeen, N. (1993). High incidence of virus diseases in Phaseolus bean in Zzanjan province. Proceedings of the 11th Plant Protection Congress of Iran, Rasht, 142.
32. Shahraeen, N., Mehraban, A. H, Mostaed, M., & Ghotbi, T. (2002). Report of bean common mosaic necrosis virus-BCMNV potyvirus infecting French bean in Aarak and Lorestan Provinces of Iran. Paper presented at the 15th Iranian Plant Protection Congress, Sept 7-11, Razi University, Kermanshah, Iran, 164.
33. Singh, S. P., Gepts, P., & Debouck, D. G. (1991). Race of common bean (Phaseolus vulgaris L. Fabaceae). Econ. Bot, 45, 379-396.
34. Tang, M., & Feng, X. (2022). Bean Common Mosaic Disease: Etiology, Resistance Resource, and Future Prospects. Agronomy, 13(1), 58.
35. Tunçtürk, F., Akköprü, A., Şensoy, S. (2019). Investigation of the effects of some root bacteria on bean blight bacteria (Xanthomonas axonopodis pv. phaseoli (Xap)) in bean (Phaseolus vulgaris L.). III. Eurasian Agricultural and Natural Sciences Congress, Antalya, Turkey, 17-20 Ekim 2019, 437-448.
36. Uçar, C., Şensoy, S. 2022. Validity control of markers used in molecular marker assisted selection in tomato. Yuzuncu Yıl University Journal of Agricultural Sciences, 32(2), 300-309.
37. Usta, M, Güller, A., Sipahioğlu, H. M. (2023). First report of ‘Candidatus Phytoplasma australasia’ strain related to witches’-broom of tomato in Türkiye. Yuzuncu Yıl University Journal of Agricultural Sciences, 33(1), 54-63.