In vitro Antifungal Activity of Mentha piperita and Thymus vulgaris Essential Oils against Ochratoxigenic Aspergillus carbonarius Isolated from Bozcaada Çavuş Grape

Year 2024, , 166 - 175, 31.03.2024

Gülçin Özcan Ateş

https://doi.org/10.29133/yyutbd.1403366

Cited By: 1

Abstract

In this study, the antifungal properties of Mentha piperita and Thymus vulgaris essential oils against an isolate of ochratoxin A producer, Aspergillus carbonarius, isolated from Bozcaada Çavuş grape, were evaluated in three steps. By GC-MS of M. piperita and T. vulgaris essential oils, the main components were determined to be menthol (39.911%) and carvacrol (49.042%). Antifungal activity was first evaluated by the agar well diffusion method, and it was determined that the tested essential oils completely inhibited the growth of A. carbonarius and were as effective as fluconazole antifungal. In the second step, the MIC and MFC values of the tested essential oils were determined; both values were 1 µL mL-1. Finally, it was determined that M. piperita and T. vulgaris essential oils completely inhibited the radial growth of A. carbonarius at the MIC value. These results show that M. piperita and T. vulgaris essential oils may be a good strategy to control ochratoxigenic A. carbonarius contamination.

Keywords

Antifungal activity, Anti-ochratoxigenic, Aspergillus carbonarius, Mentha piperita, Thymus vulgaris

Supporting Institution

This study was supported by Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit with project number FBA-2019-3028.

Project Number

FBA-2019-3028

Thanks

In this study, the identification of the isolate was supported by Çanakkale Onsekiz Mart University Scientific Research Projects Coordination with the project number FBA-2019-3028 and the antifungal activity studies were supported by the project number THD-2021-3571. The researcher funded an analysis of the volatile chemical compositions of essential oils. Regarding the use of culture, I would like to thank Dr. Nükhet Nilüfer ZORBA and Dr. Burhan ŞEN. I would like to thank Altın Toroslar A.Ş. (Adana, Türkiye) company for supplying Mentha piperita and Thymus vulgaris essential oils.

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