HSP90AB1 Variations in Four Cattle Breeds Raised in Türkiye

Abstract

Cattle play an essential role in ensuring global food security, yet they are encountering growing challenges from climate change. In Türkiye, cattle rearing is a fundamental aspect of agriculture, and native breeds are particularly important, even as their numbers dwindle. Understanding the genetic makeup of these breeds, especially their heat tolerance, is of great importance for sustainable livestock production. This study focused on identifying polymorphisms in the HSP90AB1 gene associated with heat tolerance in 122 cattle raised in Türkiye (Anatolian Black-AB, East Anatolian Red-EAR, Turkish Grey Steppe-TGS, and cosmopolitan Holstein Friesian-HF). The samples were respectively obtained from village herds in Antalya, Erzurum, Balıkesir, and Erzurum provinces for AB, TGS, EAR, and HF breeds. Blood samples were collected from different populations and genotyping was performed using Allele-Specific PCR. Regarding the HSP90AB1 gene, polymorphism was observed in the populations, with two alleles (C and G) and three genotypes (CC, CT, and TT), while the CC allele was not observed in TGS cattle. The frequency of the T allele was higher than that of the C allele in the studied cattle population. The TT genotype frequency ranged from 0.12 (HF) to 0.38 (TGS), while the lowest (0.00) and highest (0.35) CC frequencies were observed in TGS and HF, respectively. Significant deviation (p<0.05) from Hardy-Weinberg Equilibrium (HWE) was detected in the EAR and TGS cattle breeds with respect to the HSP90AB1 gene. As climate change intensifies environmental stresses, the adoption of molecular genetics and Marker-Assisted Selection (MAS) is necessary for the conservation of native genetic resources and the establishment of resilient livestock populations in Türkiye’s changing climate environment.

Keywords

• Allele-specific PCR
• Heat tolerance
• Marker-assisted selection
• Polymorphism

References

1. Atalay, S., & Kök, S. (2023). The Comparison of Polymorphisms in the Heat Shock Transcription Factor 1 Gene of Turkish Grey Cattle and Holstein Cattle. Kafkas Universitesi Veteriner Fakültesi Dergisi, 29(5), 429-435.
2. Atalay, S., Vapur, G., & Kök, S. (2024). Identification of heat stress-associated the HSPA1A (HSP70) gene in Holstein and Turkish Grey cattle. International Journal of Innovative Approaches in Agricultural Research, 8(3), 186-199.
3. Castillo, A., Gariglio, M., Franzoni, A., Soglia, D., Sartore, S., Buccioni, A., ... & Schiavone, A. (2021). Overview of native chicken breeds in Italy: conservation status and rearing systems in use. Animals, 11(2), 490.
4. Demir, E., Karsli, T., & Balcıoğlu, M.S. (2021). A comprehensive review on genetic diversity and phylogenetic relationships amongnative Turkish cattle breeds based on microsatellite markers. Turkish Journal of Veterinary & Animal Sciences, 45(1), 1-10.
5. Demir, E., Moravčíková, N., Kaya, S., Kasarda, R., Bilginer, Ü., Doğru, H., Balcioglu, M.S., & Karsli, T. (2023). Genome‐wide screening for selection signatures in native and cosmopolitan cattle breeds reared in Türkiye. Animal Genetics, 54, 721-730. doi: 10.1111/age.13361
6. Demir, E., Ceccobelli, S., Bilginer, U., Pasquini, M., Attard, G., & Karsli, T. (2022). Conservation and selection of genes related to environmental adaptation in native small ruminant breeds: a review. Ruminants, 2(2), 255-270.
7. Ertuğrul, M., Akin, A. O., Yildirim, M., Dellal, G., Togan, İ., Pabuçcuoğlu, S., Koyuncu, M., Öner, Y., Yilmaz, O., Koncagül, S., Pehlivan, E., Elmaci, C., Dağ, B., & Özder M. (2015). Türkiye çiftlik hayvanları genetik kaynaklarının korunması ve sürdürülebilir kullanımı Paper presented at the TMMOB Ziraat Mühendisleri Odası Türkiye Ziraat Mühendisliği VIII. Teknik Kongresi, 1, 212-236.
8. FAO, (2021). World food and agriculture. https://www.fao.org/3/cb4477en/cb4477en.pdf. Access date: 01.05.2024.

9. Argun Karslı, B. (2024). HSP90AB1 (SNP-4338T> C) gene polymorphism associated with thermo-tolerance in some cattle breeds in Türkiye. Mediterranean Agricultural Sciences, 37(1), 51-55.
10. Kim, H. S., Kim, J., Kim, J., & Choi, Y. H. (2022). Characterization of differential gene expression of broiler chicken to thermal stress in discrete developmental stages. Animal Cells and Systems, 26, 62-69. doi:10.1080/19768354.2022.2059566
11. Lewis Baida, B. E., Swinbourne, A. M., Barwick, J., Leu, S. T., & Wettere, W. H. (2021). Technologies for the automated collection of heat stress data in sheep. Animal Biotelemetry, 9, 1-15. doi:10.1186/s40317-020-00225-9
12. Malhi, G. S., Kaur, M., & Kaushik, P. (2021). Impact of climate change on agriculture and its mitigation strategies: A review. Sustainability, 13, 1318. doi:10.3390/su13031318
13. Onasanya, G. O., Msalya, G. M., Thiruvenkadan, A. K., Sreekumar, C., Tirumurugaan, G. K., Fafiolu, A. O., Adeleke, M. A., Yakubu, A., Ikeobi, C. O. N, & Okpeku, M. (2021). Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria. Frontiers in Genetics, 12, 391. doi: 10.3389/fgene.2021.642213
14. Öner, Y. K. A., Üstüner, H., Soysal, D., & Karakaş, V. (2017). Genetic diversity of the 3ꞌ and 5ꞌ untranslated regions of the HSP70. 1 gene between native Turkish and Holstein Friesian cattle breeds. South African Journal of Animal Science, 47(4), 424-439.
15. Peakall, R., & Smouse, P. E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28, 2537-2539. doi: 10.1093/bioinformatics/bts460
16. Prastowo, S., Adzdzakiy, M. M., Rebecca, V., Pambuko, G., & Susilowati, A. (2021). Polymorphism scanning of HSP90AB1 gene in local Friesian Holstein as molecular marker for heat stress resistance Paper presented at the First International Conference on Assessment and Development of Agricultural Innovation In E3S Web of Conferences Indonesia. doi:10.1051/e3sconf/202130605016
17. Rauw, W. M., Rydhmer, L., Kyriazakis, I., Øverland, M., Gilbert, H., Dekkers, J. C., Hermesch, S., Bouquet, A., Izquierdo, E. G., Louveau, I., & Gomez‐Raya, L. (2020). Prospects for sustainability of pig production in relation to climate change and novel feed resources. Journal of the Science of Food and Agriculture, 100, 3575-3586. doi:10.1002/jsfa.10338
18. Sahu, S., Mishra, S. R., & Kundu, A. K. (2021). Impact of thermal stress on expression dynamics of HSP60 in cardiac fibroblast cells of goat. Animal Biotechnology, 32, 327-333. doi:10.1017/s1751731111002448
19. Sajjanar, B., Deb, R., Singh, U., Kumar, S., Brahmane, M., Nirmale, A., Bal, S. K., & Minhas, P. S. (2015). Identification of SNP in HSP90AB1 and its association with the relative thermotolerance and milk production traits in Indian dairy cattle. Animal Biotechnology, 26, 45-50. doi:10.1080/10495398.2014.882846
20. Sarman, E., Gülle, K., & Sarman, A. (2021). HSP türleri ve testisler üzerinde etkili bir protein olan HSP70. Avrasya Sağlık Bilimleri Dergisi, 4, 208-214. doi:10.53493/avrasyasbd.839665
21. Skuce, P. J., Morgan, E. R., Van Dijk, J., & Mitchell, M. (2013). Animal health aspects of adaptation to climate change: beating the heat and parasites in a warming Europe. Animal, 7, 333-345. doi:10.1017/s175173111300075x
22. Suhendro, I., Noor, R. R., Jakaria, J., Priyanto, R., Manalu, W., & Andersson, G. (2024). Association of heat-shock protein 70.1 gene with physiological and physical performance of Bali cattle. Veterinary World, 17(1), 17.
23. Şahin, O., Yılmaz, İ., & Akbulut, Ö. (2023). A Research on The Milk Yield Traits of Brown Swiss, Simmental, Holstein Friesian Cattle and Their Crossbreeds Registered in The Herdbook System in Türkiye. Yuzuncu Yıl University Journal of Agricultural Sciences, 33(2), 174-182.
24. TUIK, (2023). Hayvansal Üretim İstatistikleri. https://data.tuik.gov.tr/Kategori/GetKategori?p=tarim-111&dil=1. Access date: 05.03.2024.
25. Yurdagul, K. G., Atay, S., Bilginer, Ü., Karsli, T., & Demir, E. (2023). Genetic variations in HSP90AA1 gene region in Pırlak sheep breed. Hayvansal Üretim, 64, 12-16. doi:10.29185/hayuretim.1268591